Interventional Effect and Mechanism of Saikosaponin A on Noise-induced Tinnitus in Mice

Yan ZHANG; Haojie ZHAO; Mingzhi DUAN; Jingyan DUAN; Xueyan YANG

doi:10.12259/j.issn.2095-610X.S20231105

Volume 44 Issue 11

Nov. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2023 > 44(11): 29-37

Yan ZHANG, Haojie ZHAO, Mingzhi DUAN, Jingyan DUAN, Xueyan YANG. Interventional Effect and Mechanism of Saikosaponin A on Noise-induced Tinnitus in Mice[J]. Journal of Kunming Medical University, 2023, 44(11): 29-37. doi: 10.12259/j.issn.2095-610X.S20231105

Citation:

Yan ZHANG, Haojie ZHAO, Mingzhi DUAN, Jingyan DUAN, Xueyan YANG. Interventional Effect and Mechanism of Saikosaponin A on Noise-induced Tinnitus in Mice[J]. Journal of Kunming Medical University, 2023, 44(11): 29-37. doi: 10.12259/j.issn.2095-610X.S20231105

Citation:

PDF( 2221 KB)

Interventional Effect and Mechanism of Saikosaponin A on Noise-induced Tinnitus in Mice

doi: 10.12259/j.issn.2095-610X.S20231105

1.
Dept. of Otolaryngology，Baoshan People's Hospital
2.
Dept. of Oncology，Baoshan Yunnan 678000
3.
Baoshan College of Traditional Chinese Medicine，Baoshan Yunnan 678000，China

Received Date: 2023-08-17
Publish Date: 2023-11-25

Abstract

Abstract

Objective To investigate the intervention effect and mechanism of Saikosaponin A on noise-induced tinnitus in a mouse model. Methods Fifty mice were randomly divided into control group（Control group）, noise-induced tinnitus mouse model group（BBN group）, and SSA-L group, SSA-M group and SSA-H group based on BBN group with 10 mg/kg, 20 mg/kg and 40 mg/kg of Caihu saponin A by gavage, respectively, 10 mice in each group. The tinnitus behavior of mice was detected; the depression-like behavior of mice was assessed by the open field experiment, elevated cross-maze experiment and forced swimming experiment; the hearing of mice was measured by ABR threshold; and IL-6, TNF-α, IL-1β mRNA and protein expression in the auditory cortex of mice were detected by RT-qPCR and Western blotting. Results Compared with the Control group, mice in the BBN group had significantly lower GPIAS% values at 3 d, 14 d and 28 d after 2 h of noise exposure（P < 0.05）; compared with the BBN group, mice in the SSA-L, SSA-M and SSA-H groups had significantly higher GPIAS% values at 3 d, 14 d and 28 d after 2 h of noise exposure in a dose-dependent manner（ P < 0.05）. Compared with the Control group, mice in the BBN group had significantly reduced time and distance to the central zone, number and time to the open arm, and significantly higher immobility time and ABR thresholds under Click and 8, 16 and 32 kHz acoustic stimulation（ P < 0.05）; compared with the BBN group, mice in the SSA-L, SSA-M and SSA-H groups had significantly higher time and distance to the central zone, and The number and time of entering the open arm were significantly increased, and the immobility time and Click and ABR thresholds under 8, 16 and 32 kHz acoustic stimulation were significantly decreased（ P < 0.05）. Compared with the Control group, IL-6, TNF-α, IL-1β mRNA and protein expression were significantly increased in the auditory cortex of mice in the BBN group（ P < 0.05）; compared with the BBN group, IL-6, TNF-α, IL-1β mRNA and protein expression were significantly decreased in the auditory cortex of mice in the SSA-L, SSA-M and SSA-H groups（ P < 0.05）. Conclusion Saikosaponin A can improve depressive behavior and hearing in noise-induced tinnitus mice, which may be closely related to the inhibition of inflammatory factors in the auditory cortex of tinnitus mice.
- Saikosaponin A,
- Tinnitus,
- Depressive behavior,
- Inflammatory response

FullText(HTML)

References(20)

References

[1]	Malfatti T,Ciralli B,Hilscher M M,et al. Decreasing dorsal cochlear nucleus activity ameliorates noise-induced tinnitus perception in mice[J]. BMC Biology,2022,20(1):102. doi: 10.1186/s12915-022-01288-1
[2]	Yan W,Zhu H,Yu B,et al. Effects of two inhibitors of metabolic glutamate receptor 5 on expression of endogenous homer scaffold protein 1 in the auditory cortex of mice with tinnitus[J]. Bioengineered,2021,12(1):7145-7153. doi: 10.1080/21655979.2021.1979354
[3]	Longenecker R J,Gu R,Homan J,et al. Development of tinnitus and hyperacusis in a mouse model of tobramycin cochleotoxicity[J]. Front Mol Neurosci,2021,14:715952. doi: 10.3389/fnmol.2021.715952
[4]	Adcock K,Vanneste S. Neuroinflammation in tinnitus[J]. Curr Otorhinolaryngol Rep,2022,10(3):322-328. doi: 10.1007/s40136-022-00411-8
[5]	Brüggemann P,Sória M G,Brandes-Schramm J,et al. The influence of depression,anxiety and cognition on the treatment effects of ginkgo biloba extract egb 761® in patients with tinnitus and dementia: A mediation analysis[J]. J Clin Med,2021,10(14):3151. doi: 10.3390/jcm10143151
[6]	Koch L,Gaese B H,Nowotny M. Strain comparison in rats differentiates strain-specific from more general correlates of noise-induced hearing loss and tinnitus[J]. J Assoc Res Otolaryngol,2022,23(1):59-73. doi: 10.1007/s10162-021-00822-2
[7]	Sui C,Han W J,Zhu C R,et al. Recent progress in saikosaponin biosynthesis in Bupleurum[J]. Curr Pharm Biotechnol,2021,22(3):329-340. doi: 10.2174/1389201021999200918101248
[8]	Wang X,Ming Y,Li Q,et al. Saikosaponin a alleviates rat liver fibrosis by inhibiting Hedgehog signaling pathway-mediated autophagy and NLRP3 inflammasome[J]. Trop J Pharm Res,2023,22(4):741-747. doi: 10.4314/tjpr.v22i4.5
[9]	Marinos L,Kouvaros S,Bizup B,et al. Transient delivery of a KCNQ2/3-specific channel activator 1 week after noise trauma mitigates noise-induced tinnitus[J]. J Assoc Res Otolaryngol,2021,22(2):127-139. doi: 10.1007/s10162-021-00786-3
[10]	Aliomrani M,Mesripour A,Sayahpour Z. AChR is partly responsible in mice depressive-like behavior after Phosalone exposure[J]. Neurotoxicol Teratol,2021,84:106957. doi: 10.1016/j.ntt.2021.106957
[11]	Hao J,Xia L,Yang X. The latest research progress of tinnitus related treatment[J]. Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi,2021,35(12):1141-1144.
[12]	Henton A,Tzounopoulos T. The neuroscience and the treatment of tinnitus[J]. Hear J,2021,74(6):16-17.
[13]	唐薇,凌赛泳,向澎,等. 不同强度白噪声建立噪声性耳鸣动物模型效果比较及对听皮层生长相关蛋白-43表达的影响[J]. 右江民族医学院学报,2023,45(2):259-262,286.
[14]	Sun C,Gao M,Qiao M. Research progress of traditional Chinese medicine compound" Xiaochaihu Decoction" in the treatment of depression[J]. Biomed Pharmacother,2023,159:114249. doi: 10.1016/j.biopha.2023.114249
[15]	Labree B,Hoare D J,Gascoyne L E,et al. Determining the effects of transcranial direct current stimulation on tinnitus,depression,and anxiety: A systematic review[J]. Brain Sci,2022,12(4):484. doi: 10.3390/brainsci12040484
[16]	赵慧源,田诗琪,翟春影,等. 柴胡皂苷a对抑郁模型大鼠脑内神经递质及行为学的影响[J]. 中国医学创新,2021,18(34):28-32. doi: 10.3969/j.issn.1674-4985.2021.34.007
[17]	吴启洋,刘纪明,谢华,等. 柴胡皂苷a药理作用及其作用机制的研究进展[J]. 赣南医学院学报,2022,42(11):1143-1150.
[18]	惠林娜,宋勇莉,查定军. 急性耳鸣预后及影响因素研究进展[J]. 国际耳鼻咽喉头颈外科杂志,2022,46(6):357-360.
[19]	Tetteh H,Lee M,Lau C G,et al. Tinnitus: Prospects for pharmacological interventions with a seesaw model[J]. Neuroscientist,2018,24(4):353-367. doi: 10.1177/1073858417733415
[20]	郭旭彤,安继东,梅建强. 柴胡皂苷A对抑郁症大鼠Treg和Th17免疫平衡的影响[J]. 海南医学院学报,2020,26(22):1686-1690. doi: 10.13210/j.cnki.jhmu.20200508.003

Relative Articles(20)

Relative Articles

[1]	Ji WANG, Songtao BIN, Li TAN. Study on the Efficacy of Acetylcysteine in Severe Pneumonia in Children under Electronic Bronchoscopy. Journal of Kunming Medical University, 2024, 45(6): 113-119. doi: 10.12259/j.issn.2095-610X.S20240615
[2]	Man YANG, Xingan ZHAO, Yunna GE, Juan QIN, Xiya WANG, Siming TAO. Identification of Atrial Fibrillation-related Inflammatory Genes and Their Association with Immune Cell Infiltration Based on Comprehensive Bioinformatic Analysis. Journal of Kunming Medical University, 2024, 45(3): 18-29. doi: 10.12259/j.issn.2095-610X.S20240303
[3]	Yidan FU, Wenting CHEN, Xiaoyang SU, Yan ZHAO, Danfeng LAN, Qiuping YANG. Role of Mertk-mediated NF-κb Pathway in Inflammatory Response of Schwann Cells. Journal of Kunming Medical University, 2023, 44(12): 20-24. doi: 10.12259/j.issn.2095-610X.S20231203
[4]	Yaling ZHAO, Kun WU, Kai WANG, Rong HUANG, Genya HE, Yuhui NA, Miao HE, Zhenbo DING, Caiying ZHANG. Influence of Intrauterine Hyperglycemia Environment on Peripheral Inflammatory Response in Offspring of Gestational Diabetes Mellitus. Journal of Kunming Medical University, 2023, 44(11): 70-75. doi: 10.12259/j.issn.2095-610X.S20231110
[5]	Meng CHEN, Dingyun YOU, Chenyu XING, Jinman ZHANG, Hongying LI, Zaiqing QU, Bei ZHU. Association between Morning Sickness and Depression in Pregnancy. Journal of Kunming Medical University, 2022, 43(12): 30-35. doi: 10.12259/j.issn.2095-610X.S20221208
[6]	Li-yuan AN, Bing LI, Gang SU. Effects of Different Doses of Ulinastatinon Postoperative Cognitive Function and HMGB1 in Elderly Patients. Journal of Kunming Medical University, 2021, 42(7): 121-125. doi: 10.12259/j.issn.2095-610X.S20210720
[7]	Jun-jie LI, Hai-yan JIANG, Wen-ya BAI, Si-ying HUO, Zhi-sheng SUN, Jian-lin SHAO. Effects of Silencing RND3 Expression on the Inflammatory Response and Apoptosis of Hippocampal Neurons Injured by Oxygen Glucose Deprivation/Reoxygenation. Journal of Kunming Medical University, 2021, 42(10): 14-21. doi: 10.12259/j.issn.2095-610X.S20211012
[8]	Li Jiao , Li Jiang , Fu Peng , Peng Shuang , Ruan Zhi Guo , Hou An Guo , Ke Jin . . Journal of Kunming Medical University, 2020, 41(06): 15-22.
[9]	Wang Guang , Yang Tong Xin , Jiang Yong Ming , Fang Ke Wei , Liu Jian He , Li Jiong Ming . . Journal of Kunming Medical University, 2019, 40(06): 23-27.
[10]	Na Zhu Hui , Fu Qiong Fen , Pi Jing Hong , Chen Wen Min , Miao Yong Ping , Lei Yu , Ou Yang Jing . . Journal of Kunming Medical University, 2018, 39(11): 74-78.
[11]	Chen Zhong Yi , Qiao Ting Ting , Dong Bao Lian , Guo Ling . A Study of LPS Mediated Inflammatory Oxidation Reaction of Three Types of Rodents. Journal of Kunming Medical University, 2018, 39(06): 7-13.
[12]	Zhang Nan Yang , Pan Yu , Wu Guo Dong , Zeng Yue Qin , Chen Liang . . Journal of Kunming Medical University, 2018, 39(12): 23-27.
[13]	Qu Jing Lei , Yang Yuan Zheng . Effects of Exchange Transfusion on Renal Function, Inflammatory Factors and Blood Internal Environment in Children with Hyperbilirubinemia. Journal of Kunming Medical University, 2018, 39(01): 96-99.
[14]	Wei Jiang Xia , Wang Mei , Liu Gui , Dai Xue Mei . A Study on the Effects of Cold Knife Excision by Hysteroscopy for the Local Microcirculation and Inflammatory Reaction of Patients with Hysteromyoma. Journal of Kunming Medical University, 2018, 39(05): 111-114.
[15]	Ke Jin , Cheng Xin , Hou An Guo . Preparation and in Vitro Release Study of Panax Notoginseng Sustained-release Pellets. Journal of Kunming Medical University, 2017, 38(09): 24-29.
[16]	Li Fei . Effect of Celebrex on the Hemorheology and Levels of Inflammatory Factors in Patients with Delayed Fracture Healing. Journal of Kunming Medical University,
[17]	Zhang Yang . . Journal of Kunming Medical University,
[18]	Wang Hong . . Journal of Kunming Medical University,
[19]	Jin Di . . Journal of Kunming Medical University,
[20]	Wang Hong . . Journal of Kunming Medical University,