Huashi Runjing Decoction Amelioretes the Inflammatory Response of Castrated Male Dry Eye Disease Rats by Regulating COX-2，TNF-α，and IL-6 Proteins

Jinrun YANG; Huanhuan ZHAO; Zhicong WU; Gerui YU; Lan MA; Yufen CHEN; Manyi XIE; Yu DONG

doi:10.12259/j.issn.2095-610X.S20231106

Volume 44 Issue 11

Nov. 2023

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Jinrun YANG, Huanhuan ZHAO, Zhicong WU, Gerui YU, Lan MA, Yufen CHEN, Manyi XIE, Yu DONG. Huashi Runjing Decoction Amelioretes the Inflammatory Response of Castrated Male Dry Eye Disease Rats by Regulating COX-2，TNF-α，and IL-6 Proteins[J]. Journal of Kunming Medical University, 2023, 44(11): 38-46. doi: 10.12259/j.issn.2095-610X.S20231106

Citation:

Jinrun YANG, Huanhuan ZHAO, Zhicong WU, Gerui YU, Lan MA, Yufen CHEN, Manyi XIE, Yu DONG. Huashi Runjing Decoction Amelioretes the Inflammatory Response of Castrated Male Dry Eye Disease Rats by Regulating COX-2，TNF-α，and IL-6 Proteins[J]. Journal of Kunming Medical University, 2023, 44(11): 38-46. doi: 10.12259/j.issn.2095-610X.S20231106

Citation:

Jinrun YANG, Huanhuan ZHAO, Zhicong WU, Gerui YU, Lan MA, Yufen CHEN, Manyi XIE, Yu DONG. Huashi Runjing Decoction Amelioretes the Inflammatory Response of Castrated Male Dry Eye Disease Rats by Regulating COX-2，TNF-α，and IL-6 Proteins[J]. Journal of Kunming Medical University, 2023, 44(11): 38-46. doi: 10.12259/j.issn.2095-610X.S20231106

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Huashi Runjing Decoction Amelioretes the Inflammatory Response of Castrated Male Dry Eye Disease Rats by Regulating COX-2，TNF-α，and IL-6 Proteins

doi: 10.12259/j.issn.2095-610X.S20231106

1.
Dept. of Ophthalmology，The First Affiliated Hospital of Yunnan University of Chinese Medicine，Kunming Yunnan 650021
2.
The School of Basic Medicine of Yunnan University of Chinese Medicine，Kunming Yunnan 650500

Received Date: 2023-09-13
Available Online: 2023-11-04
Publish Date: 2023-11-30

Abstract

Abstract

Objective To observe whether Huashi Runjing Decoction can inhibit the inflammatory response in rats with desmoplastic male dry eye disease, and to explore its potential mechanism of action. Methods 60 healthy male SD rats were randomly divided into 4 groups: sham surgery group of 15, negative control group of 15, positive control group of 15, Huashi Running Decoction group of 15）. The sham operated group rats only underwent abdominal incision without removing both testicles and epididymis; Rats in the negative control group, positive control group and Huashi Runjing Decoction group were removed both testicles and epididymis. After 1 week of modeling, the model was validated by fluorescein staining（FL）, break-up time（BUT）, and tear secretion test（SIT）. After the wound healed, the rats were given the appropriate drug interventions. The degree of corneal breakage, tear film rupture time and tear secretion of rats in each group were detected by FL method, BUT method and SIT method respectively after 8 weeks of drug administration. The levels of COX-2, TNF-α and IL-6 in the serum of each group of rats were observed by ELISA; HE staining was used to observe the pathomorphological changes in the lacrimal gland tissue of rats in each group. The expression of key inflammatory factors COX-2, TNF-α and IL-6 in the lacrimal gland tissues of rats in each group was observed by immunohistochemistry and Western blot. Results Compared with the sham-operated group, the negative control group, positive control group and Huashi Runjing Decoction group showed an increase in the area of sodium fluorescein positive staining in the cornea, a significant increase in FL score, a shortening of tear film rupture time and a decrease in tear secretion, an increase in serum COX-2, TNF-α and IL-6 levels, atrophy of the lacrimal gland tissue vesicles, an irregular shape, a disorganized structure, a decrease in intracytoplasmic zymogen particles, and a large number of inflammatory cell infiltrations. The expression levels of inflammatory factors COX-2, TNF-α and IL-6 increased in the tissues（P < 0.05）; Compared with the negative control group, the Huashi Runjing Decoction group showed a significant decrease in the area of sodium fluorescein positive staining, a significant decrease in FL score, a significant increase in tear film rupture time and tear secretion, a decrease in serum COX-2, TNF-α and IL-6 levels, a decrease in atrophy of the lacrimal gland tissue vesicles, a more regular shape, a clearer structure, an increase in the number of intracytoplasmic zymogen particles, an increase in inflammatory cell infiltration and a decrease in the area of lacrimal gland tissue. The expression levels of COX-2, TNF-α and IL-6 in lacrimal gland tissues were reduced（P < 0.05）. Conclusion Huashi Runjing Decoction could reduce the protein expression of COX-2, TNF-α and IL-6 in the lacrimal gland tissue of castrated male dry eye disease rats, and inhibit the inflammatory response.
- Dry eye disease,
- Huashi Runjing Decoction,
- Inflammatory cytokines,
- COX-2,
- TNF-α

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References(24)

References

[1]	Wirta D. Update on dry eye disease[J]. Jama,2022,327(23):2355-2356.
[2]	Lifei Y. Recent developments about the pathogenesis of dry eye disease: Based on immune inflammatory mechanisms [J]. Frontiers in pharmacology, 2021, 12: 732887. 补充期号 Lifei Y. Recent developments about the pathogenesis of dry eye disease: Based on immune inflammatory mechanisms[J]. Frontiers in Pharmacology,2021,12:732887.
[3]	孙子雯,崔洪玮,孙喜灵,等. 干眼病的病因、发病机制及治疗进展[J]. 山东大学耳鼻喉眼学报,2019(2):159-166. doi: 10.6040/j.issn.1673-3770.0.2018.411
[4]	邢悦,张晓梅. 干眼病因的研究进展[J]. 中国现代医学杂志,2021,31(18):49-54. doi: 10.3969/j.issn.1005-8982.2021.18.010
[5]	Borrelli M,Frings A,Geerling G,et al. Gender-specific differences in signs and symptoms of dry eye disease[J]. Curr Eye Res,2021,46(3):294-301. doi: 10.1080/02713683.2020.1801758
[6]	Eric B, Papas. The global prevalence of dry eye disease: A bayesian view[J]. Ophthalmic & Physiological Optics: The Journal of the British College of Ophthalmic Opticians (Optometrists),2021,41(6):1254-1266.
[7]	Mehra D,Galor A. Digital screen use and dry eye: A review[J]. Asia-Pacific Journal of Ophthalmology (Philadelphia,Pa),2020,9(6):491-497. doi: 10.1097/APO.0000000000000328
[8]	O'Neil E C,Henderson M,Massaro-Giordano M,et al. Advances in dry eye disease treatment[J]. Current Opinion in Ophthalmology,2019,30(3):166-178. doi: 10.1097/ICU.0000000000000569
[9]	桂炎香,李青松,赵黎,等. 干眼的中医治疗进展[J]. 现代中西医结合杂志,2020,29(9):1018-1021. doi: 10.3969/j.issn.1008-8849.2020.09.026
[10]	董玉,王鹏,张昆. 苏藩主任辨证分型治疗干眼症60例[J]. 云南中医中药杂志,2012,33(5):3-5. doi: 10.3969/j.issn.1007-2349.2012.05.002
[11]	张燕,谢军,徐艺玫,等. 去势大鼠干眼症模型眼表损伤的研究[J]. 生物医学工程研究,2013,32(2):97-100. doi: 10.3969/j.issn.1672-6278.2013.02.008
[12]	李海中,彭清华,王芬,等. 密蒙花总黄酮对去势雄鼠干眼病血清睾酮水平的影响[J]. 国际眼科杂志,2013,13(11):2174-2178. doi: 10.3980/j.issn.1672-5123.2013.11.03
[13]	中华医学会眼科学分会角膜病学组. 干眼临床诊疗专家共识(2013年)[J]. 中华眼科杂志,2013,49(1):73-75.
[14]	《中成药治疗优势病种临床应用指南》标准化项目组. 中医药治疗干眼临床应用指南(2021年)[J]. 中国中西医结合杂志,2022,42(9):1040-1046.
[15]	Ganesalingam K,Ismail S,Sherwin T,et al. Molecular evidence for the role of inflammation in dry eye disease[J]. Clinical & Experimental Optometry,2019,102(5):446-454.
[16]	Wang L,Deng Y. The applications of androgen in the treatment of dry eye disease: A systematic review of clinical studies[J]. Endocrine journal,2020,67(9):893-902. doi: 10.1507/endocrj.EJ20-0178
[17]	ruong S,Cole N,Stapleton F,et al. Sex hormones and the dry eye[J]. Clinical & Experimental Optometry,2014,97(4):324-336.
[18]	Song X,Zhao P,Wang G,Zhao X,et al. The effects of estrogen and androgen on tear secretion and matrix metalloproteinase-2 expression in lacrimal glands of ovariectomized rats[J]. Investigative Ophthalmology & Visual Science,2014,55(2):745-751.
[19]	谢翔,应炜阳,金胜威. COX-2在炎症消退中的研究进展[J]. 生命的化学,2016,36(4):461-464. doi: 10.13488/j.smhx.20160406
[20]	Kulkarni P S,Srinivasan B D. Cyclooxygenase and lipoxygenase pathways in anterior uvea and conjunctiva[J]. Progress in Clinical and Biological Research,1989,312:39-52.
[21]	Shim J,Park C,Lee H S,et al. Change in prostaglandin expression levels and synthesizing activities in dry eye disease[J]. Ophthalmology,2012,119(11):2211-2219. doi: 10.1016/j.ophtha.2012.05.038
[22]	Ji Y W,Seo Y,Choi W,et al. Dry eye-induced CCR7+CD11b+ cell lymph node homing is induced by COX-2 activities[J]. Investigative Ophthalmology & Visual Science,2014,55(10):6829-6838.
[23]	Ueta M. Genetic predisposition to stevens-johnson syndrome with severe ocular surface complications[J]. Cornea,2015,34(Suppl 11):S158-165.
[24]	马玉,李研,骆亚莉,等. 肿瘤坏死因子-α在慢性炎症与肿瘤发生中的作用[J]. 中国临床药理学杂志,2022,38(12):1419-1423. doi: 10.13699/j.cnki.1001-6821.2022.12.027

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