Zhang Mao Lin . Comparison Research between Recombinant Tissue Plasminogen Activator Intravenous Thrombolytic Therapy and Conventional Antiplatelet Therapy for Acute Cerebral Infarction[J]. Journal of Kunming Medical University, 2016, 37(03).
Citation: Ming ZHANG, Chenglong YU, Jinpeng CAO. ZNF384 Regulates Metastasis of Hepatocellular Carcinoma via GCLM[J]. Journal of Kunming Medical University, 2023, 44(11): 56-62. doi: 10.12259/j.issn.2095-610X.S20231108

ZNF384 Regulates Metastasis of Hepatocellular Carcinoma via GCLM

doi: 10.12259/j.issn.2095-610X.S20231108
  • Received Date: 2023-09-05
    Available Online: 2023-11-04
  • Publish Date: 2023-11-30
  •   Objective  To investigate the effect of ZNF384 on the proliferation and metastasis of HCC by regulating GCLM and demonstrate the specific mechanism.   Methods  si-ZNF384 and pcDNA-GCLM were transfected into HepG2 and HuH7 cell lines of HCC, respectively. Western blot was carried out to detect the expressions of ZNF384 and GCLM proteins as well as the expressions of EMT-markers N-cadherin, Vimentin and E-cadherin. CCK-8 assessed cell proliferative activity. Transwell assay was conducted to measure cell migration. Dual-luciferase experiment verified the binding relationship between ZNF384 and GCLM. The localization of ZNF384 and GCLM in HepG2 cells was detected by FISH assay.   Results  The expressions of ZNF384 and GCLM in HepG2 and HuH7 cells were increased(P < 0.05). Knockdown of ZNF384 inhibited the proliferation(P < 0.001) and migration(P < 0.001) of HepG2 and HuH7 cells, suppressed the expression of interstitial markers N-cadherin(P < 0.01) and Vimentin(P < 0.01), and promoted the expression of epithelial marker E-cadherin(P < 0.01). Database and dual-luciferase experiments confirmed that ZNF384 is bound to the promoter of GCLM. Overexpression of GCLM reversed the inhibitory effect of knocking down ZNF384 on the proliferation, migration and EMT of HepG2 and HuH7 cells(P < 0.05).   Conclusion  Knocking down ZNF384 can repress the proliferation and metastasis of HCC cells by inhibiting GCLM.
  • [1]
    Brown Z J,Tsilimigras D I,Ruff S M,et al. Management of hepatocellular carcinoma: A review[J]. JAMA Surg,2023,158(4):410-420. doi: 10.1001/jamasurg.2022.7989
    [2]
    Xie D,Shi J,Zhou J,et al. Clinical practice guidelines and real-life practice in hepatocellular carcinoma: A Chinese perspective[J]. Clin Mol Hepatol,2023,29(2):206-216. doi: 10.3350/cmh.2022.0402
    [3]
    Liu S,Liu X,Lin X,et al. Zinc finger proteins in the war on gastric cancer: Molecular mechanism and clinical potential[J]. Cells,2023,12(9):1314. doi: 10.3390/cells12091314
    [4]
    Singh J K,Smith R,Rother M B,et al. Zinc finger protein ZNF384 is an adaptor of Ku to DNA during classical non-homologous end-joining[J]. Nat Commun,2021,12(1):6560. doi: 10.1038/s41467-021-26691-0
    [5]
    Meng Q X,Wang K N,Li J H,et al. ZNF384-ZEB1 feedback loop regulates breast cancer metastasis[J]. Mol Med,2022,28(1):111. doi: 10.1186/s10020-022-00541-1
    [6]
    Yan Z,Zhou Y,Yang Y,et al. Zinc finger protein 384 enhances colorectal cancer metastasis by upregulating MMP2[J]. Oncol Rep,2022,47(3):49. doi: 10.3892/or.2022.8260
    [7]
    He L,Fan X,Li Y,et al. Overexpression of zinc finger protein 384 (ZNF 384),a poor prognostic predictor,promotes cell growth by upregulating the expression of Cyclin D1 in Hepatocellular carcinoma[J]. Cell Death Dis,2019,10(6):444. doi: 10.1038/s41419-019-1681-3
    [8]
    Yang H, Wang J, Huang Z Z, et al. Cloning and characterization of the 5'-flanking region of the rat glutamate-cysteine ligase catalytic subunit[J]. Biochem J, 2001, 357(Pt 2): 447-455.
    [9]
    Yang H,Li G,Qiu G. Bioinformatics analysis using ATAC-seq and RNA-seq for the identification of 15 gene signatures associated with the prediction of prognosis in hepatocellular carcinoma[J]. Front Oncol,2021,11:726551.
    [10]
    Tsai M C,Yen Y H,Chang K C,et al. Elevated levels of serum urokinase plasminogen activator predict poor prognosis in hepatocellular carcinoma after resection[J]. BMC Cancer,2019,19(1):1169. doi: 10.1186/s12885-019-6397-3
    [11]
    Luo Y,Teng F,Fu H,et al. Immunotherapy in liver transplantation for hepatocellular carcinoma: Pros and cons[J]. World J Gastrointest Oncol,2022,14(1):163-180. doi: 10.4251/wjgo.v14.i1.163
    [12]
    Gauthier A,Ho M. Role of sorafenib in the treatment of advanced hepatocellular carcinoma: An update[J]. Hepatol Res,2013,43(2):147-154. doi: 10.1111/j.1872-034X.2012.01113.x
    [13]
    Fang Y,Zhan Y,Xie Y,et al. Integration of glucose and cardiolipin anabolism confers radiation resistance of HCC[J]. Hepatology,2022,75(6):1386-1401. doi: 10.1002/hep.32177
    [14]
    Zhu L,Bai W,Cheng Q,et al. ZNF384-related fusion genes in acute lymphoblastic leukemia[J]. Cancer Control,2023,30:10732748231182787.
    [15]
    Grammatico S,Vitale A,La Starza R,et al. Lineage switch from pro-B acute lymphoid leukemia to acute myeloid leukemia in a case with t(12;17)(p13;q11)/TAF15-ZNF384 rearrangement[J]. Leuk Lymphoma,2013,54(8):1802-1805. doi: 10.3109/10428194.2012.753450
    [16]
    Sim J,Park J,Kim S,et al. Association of Tim-3/Gal-9 Axis with NLRC4 inflammasome in glioma malignancy: Tim-3/Gal-9 induce the NLRC4 inflammasome[J]. Int J Mol Sci,2022,23(4):2028. doi: 10.3390/ijms23042028
    [17]
    Mori S,Takeuchi T,Ishii Y,et al. Identification of APOBEC3B promoter elements responsible for activation by human papillomavirus type 16 E6[J]. Biochem Biophys Res Commun,2015,460(3):555-560. doi: 10.1016/j.bbrc.2015.03.068
    [18]
    Xiao Y,Yang K,Liu P,et al. Deoxyribonuclease 1-like 3 inhibits hepatocellular carcinoma progression by inducing apoptosis and reprogramming glucose metabolism[J]. Int J Biol Sci,2022,18(1):82-95. doi: 10.7150/ijbs.57919
    [19]
    Kendig E L,Chen Y,Krishan M,et al. Lipid metabolism and body composition in Gclm(-/-) mice[J]. Toxicol Appl Pharmacol,2011,257(3):338-348. doi: 10.1016/j.taap.2011.09.017
    [20]
    Wang T,Li C,Han B,et al. Neuroprotective effects of danshensu on rotenone-induced parkinson's disease models in vitro and in vivo[J]. BMC Complement Med Ther,2020,20(1):20. doi: 10.1186/s12906-019-2738-7
    [21]
    Wang S, Wang H, Zhu S, et al. Systematical analysis of ferroptosis regulators and identification of GCLM as a tumor promotor and immunological biomarker in bladder cancer[J]. Front Oncol, 2022, 24(12): 1040892.
    [22]
    Inoue Y,Tomisawa M,Yamazaki H,et al. The modifier subunit of glutamate cysteine ligase (GCLM) is a molecular target for amelioration of cisplatin resistance in lung cancer[J]. Int J Oncol,2003,23(5):1333-1339.
    [23]
    Wu N,Zhu D,Li J,et al. CircOMA1 modulates cabergoline resistance by downregulating ferroptosis in prolactinoma[J]. J Endocrinol Invest,2023,46(8):1573-1587. doi: 10.1007/s40618-023-02010-w
    [24]
    Cheng M L,Lu Y F,Chen H,et al. Liver expression of Nrf2-related genes in different liver diseases[J]. Hepatobiliary Pancreat Dis Int,2015,14(5):485-491. doi: 10.1016/S1499-3872(15)60425-8
  • Relative Articles

    [1] Kun ZHOU, Yali LIU, Ziliang LI, Liping QIAN, Liquan RAN, Yalan REN. Effect of miR-34a on Proliferation and Osteogenic Differentiation of Human Periodontal Stem Cells. Journal of Kunming Medical University, 2025, 46(): 1-7.
    [2] Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome. Journal of Kunming Medical University, 2024, 45(1): 15-21.  doi: 10.12259/j.issn.2095-610X.S20240103
    [3] Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells. Journal of Kunming Medical University, 2023, 44(10): 47-54.  doi: 10.12259/j.issn.2095-610X.S20231028
    [4] Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation,Proliferation and Apoptosis of Hepatic Stellate Cells. Journal of Kunming Medical University, 2023, 44(9): 7-14.  doi: 10.12259/j.issn.2095-610X.S20230926
    [5] Huajie WU, Dezhen TU, Lei LI, Han QIAO, Guoping LI. Effect of Resveratrol on Metastasis of Nasopharyngeal Carcinoma and Its Related Mechanism. Journal of Kunming Medical University, 2023, 44(2): 14-22.  doi: 10.12259/j.issn.2095-610X.S20230220
    [6] Yi FENG, Xiaofeng WANG, Ximin BAI, Sheng YAO, Juntao DANG, Yunjie ZHAO, Bing CAI. miR-149-5p Regulates Malignant Biological Behavior of Glioma Cells Through MSH5/WNT Signaling Pathway. Journal of Kunming Medical University, 2023, 44(8): 59-70.  doi: 10.12259/j.issn.2095-610X.S20230823
    [7] Zhoujun LIAO, Shaohua YANG, Lixin LIU, Sheng HU, Yihui CHEN, Qiang KANG, Xiaowen ZHANG. Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1. Journal of Kunming Medical University, 2022, 43(6): 1-6.  doi: 10.12259/j.issn.2095-610X.S20220611
    [8] Wei ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Liang ZHANG. miR-125b-5p Regulates HK2 to Inhibit Proliferation and Glycolysis of Gallbladder Cancer Cells. Journal of Kunming Medical University, 2022, 43(12): 23-29.  doi: 10.12259/j.issn.2095-610X.S20221206
    [9] Baoquan WANG, Wei ZHANG, Yuan TIAN, Xifeng LEI, Xu WANG. miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1. Journal of Kunming Medical University, 2022, 43(2): 47-53.  doi: 10.12259/j.issn.2095-610X.S20220223
    [10] Lei Xi Feng , Hou Feng Qiang , Yang Shao Hua , Zhang Wei . . Journal of Kunming Medical University, 2018, 39(12): 49-53.
    [11] Lei Xi Feng , Hou Feng Qiang , Liu Tao , Zhang Wei . . Journal of Kunming Medical University, 2018, 39(11): 11-15.
    [12] Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
    [13] Wei Dong . Effect of Fragile Site WWOX Gene on Regulating Proliferation of Human Gallbladder Cancer Cells in Vitro. Journal of Kunming Medical University,
    [14] Qiu Jian Wu . . Journal of Kunming Medical University,
    [15] Xiong Wei . . Journal of Kunming Medical University,
    [16] Wang Hai Feng . . Journal of Kunming Medical University,
    [17] Zhou JieYan . . Journal of Kunming Medical University,
    [18] Xia Ying Jie . . Journal of Kunming Medical University,
    [19] Wu Bin . . Journal of Kunming Medical University,
    [20] Li Xiong . . Journal of Kunming Medical University,
  • Cited by

    Periodical cited type(1)

    1. 唐晓静,齐丽伟,吴艳丽,刘宝军. 老年糖尿病足溃疡患者血清lncRNA H19、lncRNA GAS5表达与病情严重程度及预后的关系. 中国老年学杂志. 2024(21): 5216-5219 .

    Other cited types(0)

  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-04050100150200
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 33.0 %FULLTEXT: 33.0 %META: 65.9 %META: 65.9 %PDF: 1.1 %PDF: 1.1 %FULLTEXTMETAPDF
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 6.0 %其他: 6.0 %其他: 0.1 %其他: 0.1 %China: 0.2 %China: 0.2 %Taoyuan District: 0.0 %Taoyuan District: 0.0 %上海: 6.6 %上海: 6.6 %东莞: 0.1 %东莞: 0.1 %佛山: 0.0 %佛山: 0.0 %保定: 0.1 %保定: 0.1 %六安: 0.1 %六安: 0.1 %兰州: 0.1 %兰州: 0.1 %凉山: 0.1 %凉山: 0.1 %北京: 42.4 %北京: 42.4 %十堰: 0.1 %十堰: 0.1 %南京: 0.1 %南京: 0.1 %南宁: 0.1 %南宁: 0.1 %南通: 0.2 %南通: 0.2 %博阿努瓦: 0.0 %博阿努瓦: 0.0 %合肥: 0.0 %合肥: 0.0 %呼伦贝尔: 0.0 %呼伦贝尔: 0.0 %呼和浩特: 0.0 %呼和浩特: 0.0 %咸阳: 0.0 %咸阳: 0.0 %哈尔滨: 0.0 %哈尔滨: 0.0 %哥伦布: 0.1 %哥伦布: 0.1 %嘉兴: 0.1 %嘉兴: 0.1 %嘉峪关: 0.0 %嘉峪关: 0.0 %坦佩: 0.1 %坦佩: 0.1 %天津: 0.7 %天津: 0.7 %安那罕: 0.7 %安那罕: 0.7 %宣城: 0.1 %宣城: 0.1 %宿州: 0.1 %宿州: 0.1 %广安: 0.0 %广安: 0.0 %广州: 0.0 %广州: 0.0 %庆阳: 0.1 %庆阳: 0.1 %张家口: 1.0 %张家口: 1.0 %德宏: 0.1 %德宏: 0.1 %惠州: 0.0 %惠州: 0.0 %成都: 0.2 %成都: 0.2 %扬州: 0.1 %扬州: 0.1 %无锡: 0.3 %无锡: 0.3 %昆明: 1.4 %昆明: 1.4 %晋城: 0.0 %晋城: 0.0 %景德镇: 0.0 %景德镇: 0.0 %曲靖: 0.0 %曲靖: 0.0 %朝阳: 0.1 %朝阳: 0.1 %杭州: 0.3 %杭州: 0.3 %松原: 0.1 %松原: 0.1 %格兰特县: 0.0 %格兰特县: 0.0 %武汉: 0.1 %武汉: 0.1 %沈阳: 0.1 %沈阳: 0.1 %河内: 0.3 %河内: 0.3 %泰州: 0.0 %泰州: 0.0 %洛阳: 0.1 %洛阳: 0.1 %海得拉巴: 0.0 %海得拉巴: 0.0 %淄博: 0.0 %淄博: 0.0 %淮安: 0.0 %淮安: 0.0 %深圳: 0.1 %深圳: 0.1 %温州: 0.1 %温州: 0.1 %漯河: 1.4 %漯河: 1.4 %玉林: 0.0 %玉林: 0.0 %石家庄: 0.3 %石家庄: 0.3 %芒廷维尤: 9.0 %芒廷维尤: 9.0 %芝加哥: 0.4 %芝加哥: 0.4 %苏州: 0.7 %苏州: 0.7 %襄阳: 0.0 %襄阳: 0.0 %西宁: 9.7 %西宁: 9.7 %西安: 0.1 %西安: 0.1 %西雅图: 0.0 %西雅图: 0.0 %贵阳: 0.1 %贵阳: 0.1 %赤峰: 0.0 %赤峰: 0.0 %运城: 0.1 %运城: 0.1 %邯郸: 0.1 %邯郸: 0.1 %郑州: 0.4 %郑州: 0.4 %重庆: 0.1 %重庆: 0.1 %长春: 0.0 %长春: 0.0 %长沙: 0.5 %长沙: 0.5 %青岛: 0.3 %青岛: 0.3 %马赛: 0.4 %马赛: 0.4 %驻马店: 12.3 %驻马店: 12.3 %其他其他ChinaTaoyuan District上海东莞佛山保定六安兰州凉山北京十堰南京南宁南通博阿努瓦合肥呼伦贝尔呼和浩特咸阳哈尔滨哥伦布嘉兴嘉峪关坦佩天津安那罕宣城宿州广安广州庆阳张家口德宏惠州成都扬州无锡昆明晋城景德镇曲靖朝阳杭州松原格兰特县武汉沈阳河内泰州洛阳海得拉巴淄博淮安深圳温州漯河玉林石家庄芒廷维尤芝加哥苏州襄阳西宁西安西雅图贵阳赤峰运城邯郸郑州重庆长春长沙青岛马赛驻马店

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(3)

    Article Metrics

    Article views (1584) PDF downloads(18) Cited by(1)
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return