ZNF384 Regulates Metastasis of Hepatocellular Carcinoma via GCLM

Ming ZHANG; Chenglong YU; Jinpeng CAO

doi:10.12259/j.issn.2095-610X.S20231108

Volume 44 Issue 11

Nov. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2023 > 44(11): 56-62

Ming ZHANG, Chenglong YU, Jinpeng CAO. ZNF384 Regulates Metastasis of Hepatocellular Carcinoma via GCLM[J]. Journal of Kunming Medical University, 2023, 44(11): 56-62. doi: 10.12259/j.issn.2095-610X.S20231108

Citation:

Ming ZHANG, Chenglong YU, Jinpeng CAO. ZNF384 Regulates Metastasis of Hepatocellular Carcinoma via GCLM[J]. Journal of Kunming Medical University, 2023, 44(11): 56-62. doi: 10.12259/j.issn.2095-610X.S20231108

Citation:

PDF( 2364 KB)

ZNF384 Regulates Metastasis of Hepatocellular Carcinoma via GCLM

doi: 10.12259/j.issn.2095-610X.S20231108

1.
Dept. of Clinical Laboratory，Shinan District People’s Hospital of Qingdao
2.
Health Management Center Affiliated Qingdao Third People’s Hospital，Qingdao University，Shandong Qingdao 266000，China

Received Date: 2023-09-05
Available Online: 2023-11-04
Publish Date: 2023-11-30

Abstract

Abstract

Objective To investigate the effect of ZNF384 on the proliferation and metastasis of HCC by regulating GCLM and demonstrate the specific mechanism. Methods si-ZNF384 and pcDNA-GCLM were transfected into HepG2 and HuH7 cell lines of HCC, respectively. Western blot was carried out to detect the expressions of ZNF384 and GCLM proteins as well as the expressions of EMT-markers N-cadherin, Vimentin and E-cadherin. CCK-8 assessed cell proliferative activity. Transwell assay was conducted to measure cell migration. Dual-luciferase experiment verified the binding relationship between ZNF384 and GCLM. The localization of ZNF384 and GCLM in HepG2 cells was detected by FISH assay. Results The expressions of ZNF384 and GCLM in HepG2 and HuH7 cells were increased（P < 0.05）. Knockdown of ZNF384 inhibited the proliferation（P < 0.001） and migration（P < 0.001） of HepG2 and HuH7 cells, suppressed the expression of interstitial markers N-cadherin（P < 0.01） and Vimentin（P < 0.01）, and promoted the expression of epithelial marker E-cadherin（P < 0.01）. Database and dual-luciferase experiments confirmed that ZNF384 is bound to the promoter of GCLM. Overexpression of GCLM reversed the inhibitory effect of knocking down ZNF384 on the proliferation, migration and EMT of HepG2 and HuH7 cells（P < 0.05）. Conclusion Knocking down ZNF384 can repress the proliferation and metastasis of HCC cells by inhibiting GCLM.
- Hepatocellular carcinoma,
- ZNF384,
- GCLM,
- Proliferation,
- Metastasis

FullText(HTML)

References(24)

References

[1]	Brown Z J,Tsilimigras D I,Ruff S M,et al. Management of hepatocellular carcinoma: A review[J]. JAMA Surg,2023,158(4):410-420. doi: 10.1001/jamasurg.2022.7989
[2]	Xie D,Shi J,Zhou J,et al. Clinical practice guidelines and real-life practice in hepatocellular carcinoma: A Chinese perspective[J]. Clin Mol Hepatol,2023,29(2):206-216. doi: 10.3350/cmh.2022.0402
[3]	Liu S,Liu X,Lin X,et al. Zinc finger proteins in the war on gastric cancer: Molecular mechanism and clinical potential[J]. Cells,2023,12(9):1314. doi: 10.3390/cells12091314
[4]	Singh J K,Smith R,Rother M B,et al. Zinc finger protein ZNF384 is an adaptor of Ku to DNA during classical non-homologous end-joining[J]. Nat Commun,2021,12(1):6560. doi: 10.1038/s41467-021-26691-0
[5]	Meng Q X,Wang K N,Li J H,et al. ZNF384-ZEB1 feedback loop regulates breast cancer metastasis[J]. Mol Med,2022,28(1):111. doi: 10.1186/s10020-022-00541-1
[6]	Yan Z,Zhou Y,Yang Y,et al. Zinc finger protein 384 enhances colorectal cancer metastasis by upregulating MMP2[J]. Oncol Rep,2022,47(3):49. doi: 10.3892/or.2022.8260
[7]	He L,Fan X,Li Y,et al. Overexpression of zinc finger protein 384 (ZNF 384),a poor prognostic predictor,promotes cell growth by upregulating the expression of Cyclin D1 in Hepatocellular carcinoma[J]. Cell Death Dis,2019,10(6):444. doi: 10.1038/s41419-019-1681-3
[8]	Yang H, Wang J, Huang Z Z, et al. Cloning and characterization of the 5'-flanking region of the rat glutamate-cysteine ligase catalytic subunit[J]. Biochem J, 2001, 357（Pt 2）: 447-455.
[9]	Yang H,Li G,Qiu G. Bioinformatics analysis using ATAC-seq and RNA-seq for the identification of 15 gene signatures associated with the prediction of prognosis in hepatocellular carcinoma[J]. Front Oncol,2021,11:726551.
[10]	Tsai M C,Yen Y H,Chang K C,et al. Elevated levels of serum urokinase plasminogen activator predict poor prognosis in hepatocellular carcinoma after resection[J]. BMC Cancer,2019,19(1):1169. doi: 10.1186/s12885-019-6397-3
[11]	Luo Y,Teng F,Fu H,et al. Immunotherapy in liver transplantation for hepatocellular carcinoma: Pros and cons[J]. World J Gastrointest Oncol,2022,14(1):163-180. doi: 10.4251/wjgo.v14.i1.163
[12]	Gauthier A,Ho M. Role of sorafenib in the treatment of advanced hepatocellular carcinoma: An update[J]. Hepatol Res,2013,43(2):147-154. doi: 10.1111/j.1872-034X.2012.01113.x
[13]	Fang Y,Zhan Y,Xie Y,et al. Integration of glucose and cardiolipin anabolism confers radiation resistance of HCC[J]. Hepatology,2022,75(6):1386-1401. doi: 10.1002/hep.32177
[14]	Zhu L,Bai W,Cheng Q,et al. ZNF384-related fusion genes in acute lymphoblastic leukemia[J]. Cancer Control,2023,30:10732748231182787.
[15]	Grammatico S,Vitale A,La Starza R,et al. Lineage switch from pro-B acute lymphoid leukemia to acute myeloid leukemia in a case with t(12;17)(p13;q11)/TAF15-ZNF384 rearrangement[J]. Leuk Lymphoma,2013,54(8):1802-1805. doi: 10.3109/10428194.2012.753450
[16]	Sim J,Park J,Kim S,et al. Association of Tim-3/Gal-9 Axis with NLRC4 inflammasome in glioma malignancy: Tim-3/Gal-9 induce the NLRC4 inflammasome[J]. Int J Mol Sci,2022,23(4):2028. doi: 10.3390/ijms23042028
[17]	Mori S,Takeuchi T,Ishii Y,et al. Identification of APOBEC3B promoter elements responsible for activation by human papillomavirus type 16 E6[J]. Biochem Biophys Res Commun,2015,460(3):555-560. doi: 10.1016/j.bbrc.2015.03.068
[18]	Xiao Y,Yang K,Liu P,et al. Deoxyribonuclease 1-like 3 inhibits hepatocellular carcinoma progression by inducing apoptosis and reprogramming glucose metabolism[J]. Int J Biol Sci,2022,18(1):82-95. doi: 10.7150/ijbs.57919
[19]	Kendig E L,Chen Y,Krishan M,et al. Lipid metabolism and body composition in Gclm(-/-) mice[J]. Toxicol Appl Pharmacol,2011,257(3):338-348. doi: 10.1016/j.taap.2011.09.017
[20]	Wang T,Li C,Han B,et al. Neuroprotective effects of danshensu on rotenone-induced parkinson's disease models in vitro and in vivo[J]. BMC Complement Med Ther,2020,20(1):20. doi: 10.1186/s12906-019-2738-7
[21]	Wang S, Wang H, Zhu S, et al. Systematical analysis of ferroptosis regulators and identification of GCLM as a tumor promotor and immunological biomarker in bladder cancer[J]. Front Oncol, 2022, 24（12）: 1040892.
[22]	Inoue Y,Tomisawa M,Yamazaki H,et al. The modifier subunit of glutamate cysteine ligase (GCLM) is a molecular target for amelioration of cisplatin resistance in lung cancer[J]. Int J Oncol,2003,23(5):1333-1339.
[23]	Wu N,Zhu D,Li J,et al. CircOMA1 modulates cabergoline resistance by downregulating ferroptosis in prolactinoma[J]. J Endocrinol Invest,2023,46(8):1573-1587. doi: 10.1007/s40618-023-02010-w
[24]	Cheng M L,Lu Y F,Chen H,et al. Liver expression of Nrf2-related genes in different liver diseases[J]. Hepatobiliary Pancreat Dis Int,2015,14(5):485-491. doi: 10.1016/S1499-3872(15)60425-8

Relative Articles(20)

Relative Articles

[1]	Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103
[2]	Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells. Journal of Kunming Medical University, 2023, 44(10): 47-54. doi: 10.12259/j.issn.2095-610X.S20231028
[3]	Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926
[4]	Huajie WU, Dezhen TU, Lei LI, Han QIAO, Guoping LI. Effect of Resveratrol on Metastasis of Nasopharyngeal Carcinoma and Its Related Mechanism. Journal of Kunming Medical University, 2023, 44(2): 14-22. doi: 10.12259/j.issn.2095-610X.S20230220
[5]	Yi FENG, Xiaofeng WANG, Ximin BAI, Sheng YAO, Juntao DANG, Yunjie ZHAO, Bing CAI. miR-149-5p Regulates Malignant Biological Behavior of Glioma Cells Through MSH5/WNT Signaling Pathway. Journal of Kunming Medical University, 2023, 44(8): 59-70. doi: 10.12259/j.issn.2095-610X.S20230823
[6]	Wei ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Liang ZHANG. miR-125b-5p Regulates HK2 to Inhibit Proliferation and Glycolysis of Gallbladder Cancer Cells. Journal of Kunming Medical University, 2022, 43(12): 23-29. doi: 10.12259/j.issn.2095-610X.S20221206
[7]	Zhoujun LIAO, Shaohua YANG, Lixin LIU, Sheng HU, Yihui CHEN, Qiang KANG, Xiaowen ZHANG. Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1. Journal of Kunming Medical University, 2022, 43(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20220611
[8]	Baoquan WANG, Wei ZHANG, Yuan TIAN, Xifeng LEI, Xu WANG. miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1. Journal of Kunming Medical University, 2022, 43(2): 47-53. doi: 10.12259/j.issn.2095-610X.S20220223
[9]	Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504
[10]	Lei Xi Feng , Hou Feng Qiang , Yang Shao Hua , Zhang Wei . . Journal of Kunming Medical University, 2018, 39(12): 49-53.
[11]	Lei Xi Feng , Hou Feng Qiang , Liu Tao , Zhang Wei . . Journal of Kunming Medical University, 2018, 39(11): 11-15.
[12]	Wei Dong . Effect of Fragile Site WWOX Gene on Regulating Proliferation of Human Gallbladder Cancer Cells in Vitro. Journal of Kunming Medical University,
[13]	Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
[14]	Qiu Jian Wu . . Journal of Kunming Medical University,
[15]	Xiong Wei . . Journal of Kunming Medical University,
[16]	Wang Hai Feng . . Journal of Kunming Medical University,
[17]	Zhou JieYan . . Journal of Kunming Medical University,
[18]	Wu Bin . . Journal of Kunming Medical University,
[19]	Xia Ying Jie . . Journal of Kunming Medical University,
[20]	Li Xiong . . Journal of Kunming Medical University,