Effect of Median Nerve Electrical Stimulation on Synaptic Plasticity in Ischemic Stroke Rats

Chengcai ZHANG; Rong NING; Na CHEN; Yichen PENG; Li ZHOU; xichen YANG; Jingyi LU; Pengyue ZHANG; Rui LI

doi:10.12259/j.issn.2095-610X.S20231201

Volume 44 Issue 12

Dec. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(12): 6-12

Chengcai ZHANG, Rong NING, Na CHEN, Yichen PENG, Li ZHOU, xichen YANG, Jingyi LU, Pengyue ZHANG, Rui LI. Effect of Median Nerve Electrical Stimulation on Synaptic Plasticity in Ischemic Stroke Rats[J]. Journal of Kunming Medical University, 2023, 44(12): 6-12. doi: 10.12259/j.issn.2095-610X.S20231201

Citation:

Chengcai ZHANG, Rong NING, Na CHEN, Yichen PENG, Li ZHOU, xichen YANG, Jingyi LU, Pengyue ZHANG, Rui LI. Effect of Median Nerve Electrical Stimulation on Synaptic Plasticity in Ischemic Stroke Rats[J]. Journal of Kunming Medical University, 2023, 44(12): 6-12. doi: 10.12259/j.issn.2095-610X.S20231201

Citation:

Chengcai ZHANG, Rong NING, Na CHEN, Yichen PENG, Li ZHOU, xichen YANG, Jingyi LU, Pengyue ZHANG, Rui LI. Effect of Median Nerve Electrical Stimulation on Synaptic Plasticity in Ischemic Stroke Rats[J]. Journal of Kunming Medical University, 2023, 44(12): 6-12. doi: 10.12259/j.issn.2095-610X.S20231201

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Effect of Median Nerve Electrical Stimulation on Synaptic Plasticity in Ischemic Stroke Rats

doi: 10.12259/j.issn.2095-610X.S20231201

1.
The 2nd Clinical School，Yunnan University of Traditional Chinese Medicine，Kunming Yunnan 650500
2.
Dept. of Rehabilitation Medicine，The 2nd People’ s Hospital of Kunming City，Kunming Yunnan 650032，China

Received Date: 2023-09-25
Available Online: 2023-12-15
Publish Date: 2023-12-25

Abstract

Abstract

Objective To investigate the effects and mechanisms of median nerve electrical stimulation on synaptic plasticity in ischemic stroke rats. Methods 18 healthy male SD rats were randomly divided into Sham group（n = 6）, ischemic stroke group（MCAO group, n = 6） and median nerve electrical stimulation group（MNES group, n = 6）. The left middle cerebral artery occlusion model of rats was established by thread plug method. Thread plug was not inserted in sham group. The median nerve electrical stimulation group was given median nerve electrical stimulation intervention on the 3rd day after modeling, and intervention on the next day. After intervention for 7 times, behavioral detection, HE staining was used to detect median nerve injury. Nissl staining was used to detect cerebral infarction volume. Western blot was used for detection of the expression level of proteins related to synaptic plasticity, and electron microscopy was performed. Results HE staining showed that median nerve electrical stimulation did not cause damage to the median nerve in stroke rats, and the median nerve membrane was intact without obvious inflammatory cells. Compared with MCAO group, the neural function, motor function and coordination of the injured forelimb in MNES group were significantly improved（P < 0.01）. Compared with MCAO group, cerebral infarction volume in MNES group was significantly reduced（P < 0.05）, the pyknosis of Nissl bodies in ischemic penumbra decreased. Compared with MCAO group, the expression levels of synaptic plastication-related proteins PSD95 and synI in the cortex of MNES group were significantly up-regulated after median nerve electrical stimulation（P < 0.05）, the number of synapses in the ischemic cortex increased significantly（P < 0.01）. Conclusion Median nerve electrical stimulation is a safe and effective therapeutic measure to improve nerve function after stroke, and its mechanism is related to promoting synaptic plasticity.
- Median nerve electrical stimulation,
- Ischemic stroke,
- Synaptic plasticity

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References(16)

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