miR-218-5p Inhibits the Development of Colon Cancer by Regulating LAYN

Bing CAI; Wei ZHANG; Jing LIU; Yi LIU

doi:10.12259/j.issn.2095-610X.S20231206

Volume 44 Issue 12

Dec. 2023

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Bing CAI, Wei ZHANG, Jing LIU, Yi LIU. miR-218-5p Inhibits the Development of Colon Cancer by Regulating LAYN[J]. Journal of Kunming Medical University, 2023, 44(12): 32-40. doi: 10.12259/j.issn.2095-610X.S20231206

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Bing CAI, Wei ZHANG, Jing LIU, Yi LIU. miR-218-5p Inhibits the Development of Colon Cancer by Regulating LAYN[J]. Journal of Kunming Medical University, 2023, 44(12): 32-40. doi: 10.12259/j.issn.2095-610X.S20231206

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miR-218-5p Inhibits the Development of Colon Cancer by Regulating LAYN

doi: 10.12259/j.issn.2095-610X.S20231206

1.
Dept. of Pathology
2.
Dept. of General Surgery，Weinan Central Hospital，Weinan Shaanxi 714000
3.
Dept. of Oncology，Shaanxi Provincial People's Hospital，Xi’an Shaanxi 710068，China

Received Date: 2023-09-10
Available Online: 2023-12-20
Publish Date: 2023-12-25

Abstract

Abstract

Objective To investigate the expression level of miR-218-5p and LAYN in colon cancer and the effects of their regulatory relationship on the progress of colon cancer. Methods RT-qPCR was used to detect the expression of miR-218-5p in colon cancer tissues, adjacent tissues, and cell lines. Western blot was used to detect the expression level of LAYN protein in colon cancer tissues, adjacent tissues, and cell lines. The in situ hybridization（ISH） was used to detect the expression of miR-218-5p in colon cancer and adjacent tissues. Immunohistochemical（IHC） experiment was used to detect the distribution and expression of LAYN protein in colon cancer tissues and adjacent tissues. The dual luciferase experiment was used to detect the binding relationship between miR-218-5p and 3'- rn-translation region（UTR） of LAYN. CCK-8 was used to detect the cell proliferation. Transwell was used to detect the invasion ability of cells. The flow cytometry was used to detect the apoptosis level of cells. Western blot was used to detect the expression level of cleaved Caspase3 in cells. Results The expression of miR-218-5p decreased in colon cancer tissues and colon cancer cell lines, with the lowest expression in HT-29 cells（P < 0.0001）, and the expression of LAYN increased and the highest expression in HT-29 cells（P < 0.05）. The double luciferase reporter gene experiment（P < 0.01） combined with Western blot experiment（P < 0.01） showed that miR-218-5p could bind to the 3'UTR region of LAYN and inhibit its protein expression. In cell experiments, overexpression of miR-218-5p inhibited cell proliferation（P < 0.05）, invasion（P < 0.001）, and increased apoptosis（P < 0.01）, and the expression of cleaved Caspase3 protein（P < 0.01）. Overexpression of LAYN promoted cell proliferation（P < 0.05）, invasion（P < 0.001）, and decreased apoptosis（P < 0.0001） and the expression of cleaved Caspase3 protein（P < 0.01）, while overexpression of miR-218-5p and LAYN partially reversed the above changes. Conclusion The expression level of miR-218-5p is decreased in colon cancer tissues. Cell experiments show that miR-218-5p inhibits cell invasion and proliferation and promotes cell apoptosis through targeted regulation of LAYN.
- Colon cancer,
- miR-218-5p,
- LAYN,
- Invasion,
- Proliferation,
- Apoptosis

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References(20)

References

[1]	顾晋,汪建平. 中国结直肠癌诊疗规范(2023版)[J]. 协和医学杂志,2023,14(4):706-733.
[2]	Sung H,Ferlay J,Siegel R L,et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2021,71(3):209-249. doi: 10.3322/caac.21660
[3]	Yang Y,Meng W J,Wang Z Q. MicroRNAs in colon and rectal cancer - novel biomarkers from diagnosis to therapy[J]. Endocr Metab Immune Disord Drug Targets,2020,20(8):1211-1226. doi: 10.2174/1871530320666200506075219
[4]	Garo L P,Ajay A K,Fujiwara M,et al. MicroRNA-146a limits tumorigenic inflammation in colorectal cancer[J]. Nat Commun,2021,12(1):2419. doi: 10.1038/s41467-021-22641-y
[5]	Zhou X G,Huang X L,Liang S Y,et al. Identifying miRNA and gene modules of colon cancer associated with pathological stage by weighted gene co-expression network analysis[J]. Onco Targets Ther,2018,11:2815-2830. doi: 10.2147/OTT.S163891
[6]	Tammi M I,Oikari S,Pasonen-Seppänen S,et al. Activated hyaluronan metabolism in the tumor matrix - causes and consequences[J]. Matrix Biol,2019,78-79:147-164. doi: 10.1016/j.matbio.2018.04.012
[7]	Pan J H,Zhou H,Cooper L,et al. LAYN is a prognostic biomarker and correlated with immune infiltrates in gastric and colon cancers[J]. Front Immunol,2019,10:6. doi: 10.3389/fimmu.2019.00006
[8]	李哲轩, 张阳, 周彤, 等. 2020全球癌症统计报告解读[J]. 2021, 7（2）: 14.
[9]	Iacona J R, Lutz C S. miR‐146a‐5p: Expression, regulation, and functions in cancer[J], 2019, 10（4）: e1533.
[10]	Huang X,Zhu X,Yu Y,et al. Dissecting miRNA signature in colorectal cancer progression and metastasis[J]. Cancer Lett,2021,501:66-82. doi: 10.1016/j.canlet.2020.12.025
[11]	Payandeh Z,Khalili S,Somi M H,et al. PD-1/PD-L1-dependent immune response in colorectal cancer[J]. J Cell Physiol,2020,235(7-8):5461-5475. doi: 10.1002/jcp.29494
[12]	Wang H. MicroRNAs and apoptosis in colorectal cancer[J]. Int J Mol Sci,2020,21(15):5353. doi: 10.3390/ijms21155353
[13]	Zhang N,Hu X,Du Y,et al. The role of miRNAs in colorectal cancer progression and chemoradiotherapy[J]. Biomed Pharmacother,2021,134:111099. doi: 10.1016/j.biopha.2020.111099
[14]	Ilm K,Fuchs S,Mudduluru G,et al. MACC1 is post-transcriptionally regulated by miR-218 in colorectal cancer[J]. Oncotarget,2016,7(33):53443-53458. doi: 10.18632/oncotarget.10803
[15]	Yu H,Gao G,Jiang L,et al. Decreased expression of miR-218 is associated with poor prognosis in patients with colorectal cancer[J]. Int J Clin Exp Pathol,2013,6(12):2904-2911.
[16]	Deng M,Zeng C,Lu X,et al. miR-218 suppresses gastric cancer cell cycle progression through the CDK6/Cyclin D1/E2F1 axis in a feedback loop[J]. Cancer Lett,2017,403:175-185. doi: 10.1016/j.canlet.2017.06.006
[17]	Pu Y,Wei J,Wu Y,et al. THUMPD3-AS1 facilitates cell growth and aggressiveness by the miR-218-5p/SKAP1 axis in colorectal cancer[J]. Cell Biochem Biophys,2022,80(3):483-494. doi: 10.1007/s12013-022-01074-4
[18]	Liu Q Z,Yu H R,Wang L P,et al. Up-regulation of PUM1 by miR-218-5p promotes colorectal tumor-initiating cell properties and tumorigenesis by regulating the PI3K/AKT axis[J]. J Gastrointest Oncol,2023,14(1):233-244. doi: 10.21037/jgo-23-6
[19]	Liu M,Yin K,Guo X,et al. Diphthamide biosynthesis 1 is a novel oncogene in colorectal cancer cells and is regulated by miR-218-5p[J]. Cell Physiol Biochem,2017,44(2):505-514. doi: 10.1159/000485087
[20]	De Simone M,Arrigoni A,Rossetti G,et al. Transcriptional landscape of human tissue lymphocytes unveils uniqueness of tumor-infiltrating T regulatory cells[J]. Immunity,2016,45(5):1135-1147. doi: 10.1016/j.immuni.2016.10.021

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