Diagnostic Efficacy of Blood Inicators in the Diagnosis and Treatment of Gram-positive Cocci and Gram-negative Bacilli Infection

Yanbi ZHANG; Lei FENG; Rui SHI; Beibei LUO; Lingtong TANG; Huiying CAO; Qianye BI

doi:10.12259/j.issn.2095-610X.S20231218

Volume 44 Issue 12

Dec. 2023

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Yanbi ZHANG, Lei FENG, Rui SHI, Beibei LUO, Lingtong TANG, Huiying CAO, Qianye BI. Diagnostic Efficacy of Blood Inicators in the Diagnosis and Treatment of Gram-positive Cocci and Gram-negative Bacilli Infection[J]. Journal of Kunming Medical University, 2023, 44(12): 111-120. doi: 10.12259/j.issn.2095-610X.S20231218

Citation:

Yanbi ZHANG, Lei FENG, Rui SHI, Beibei LUO, Lingtong TANG, Huiying CAO, Qianye BI. Diagnostic Efficacy of Blood Inicators in the Diagnosis and Treatment of Gram-positive Cocci and Gram-negative Bacilli Infection[J]. Journal of Kunming Medical University, 2023, 44(12): 111-120. doi: 10.12259/j.issn.2095-610X.S20231218

Citation:

Yanbi ZHANG, Lei FENG, Rui SHI, Beibei LUO, Lingtong TANG, Huiying CAO, Qianye BI. Diagnostic Efficacy of Blood Inicators in the Diagnosis and Treatment of Gram-positive Cocci and Gram-negative Bacilli Infection[J]. Journal of Kunming Medical University, 2023, 44(12): 111-120. doi: 10.12259/j.issn.2095-610X.S20231218

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Diagnostic Efficacy of Blood Inicators in the Diagnosis and Treatment of Gram-positive Cocci and Gram-negative Bacilli Infection

doi: 10.12259/j.issn.2095-610X.S20231218

1.
School of Clinical and Public Health，Honghe Health Vocational College，Mengzi Yunnan 661199
2.
Dept. of Laboratory，Yan’an Hospital Affiliated to Kunming Medical University，Kunming Yunnan 650051
3.
Dept. of Clinical Laboratory，Yuxi People’s Hospital， Yuxi Yunnan 653100
4.
Laboratory of Yuxi Central Blood Station， Yuxi Yunnan 653100，China

Received Date: 2023-08-17
Available Online: 2023-12-19
Publish Date: 2023-12-25

Abstract

Abstract

Objective To explore the differences in the effectiveness of using different blood indicators individually, in combination, and for dynamic monitoring in the diagnosis, differential diagnosis, and prognosis of bacterial infections. Methods 1843 cases with infectious symptoms or signs from January 2015 to September 2022 at the People’ s Hospital of Yuxi City were selected as the case group, and 2298 uninfected individuals during the same period were selected as the control group. Blood indicators of the two groups were collected. Variables were grouped according to gender, age group, specimen type, etc. SPSS 24.0 and Medcalc 20.0 were used for statistical analysis. Results The individual diagnostic efficacy of various blood indicators for detecting infection ranges from 0.656 to 0.937. When used together, the efficacy ranges from 0.907 to 0.987. The efficacy of distinguishing between G+c and G-b in different specimens is as follows: when PCT is used alone in blood, the AUC is 0.875 for males and 0.769 for females. However, the individual diagnostic efficacy in male mucous secretions, sterile body fluids, and non-adult male sputum is all ≤0.7. Yet, when used together, the efficacy is AUC（0.789, 0.737, 0.86） respectively. The dynamic monitoring of PCT, IL-6, CRP, WBC, and LAC in adult patients at 24 h, 48 h, and 72 h after admission shows statistically significant differences in prognostic efficacy for G+c and G-b（P < 0.05）. Conclusions Blood indicators have a certain diagnostic value for determining whether there is a bacterial infection, and there are gender differences. The combined use of these indicators is more effective. The diagnostic value of using blood indicators alone or in combination for distinguishing between G+c and G-b in different types of specimens varies. The use of PCT alone in blood specimens is the most effective. For adult males, the combined use of body surface mucous secretions and sterile body fluids is most effective, while for underage males, the combined use of sputum is most effective. The combined use for females is not effective. Dynamic monitoring of PCT, CRP, IL-6, LAC, and WBC has a high value for evaluating the prognosis and therapeutic effect of infections. The evaluation of G+c infection is most effective at 24 hours for IL-6, and for G-b infection, it is most effective at 72 hours for PCT.
- Bacterial infection,
- Blood indicators,
- Difference,
- Joint detection,
- Diagnostic efficiency

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References(24)

References

[1]	Xie J,Wang H,Kang Y,et al. The epidemiology of sepsis in Chinese ICUs: A national cross-sectional survey[J]. Critcaremed,2020,48(3):e209-e218.
[2]	宋健梅,邓德耀,袁文丽,等. 血清降钙素原在成人细菌性血流感染中的早期预测价值[J]. 中国抗生素杂志,2021,46(7):717-720.
[3]	朱美英,曹鄂洪. 降钙素原的检测和应用—《感染相关生物标志物临床意义解读专家共识》解读[J]. 上海医药,2018,39(1):14-18.
[4]	中国医师协会急诊医师分会,中华医学会急诊医学分会,中国急诊专科医联体,北京急诊医学学会. 急诊成人细菌性感染诊疗专家共识[J]. 中华急诊医学杂志,2020,29(11):1388-1395.
[5]	降钙素原在成人下呼吸道感染性疾病分级管理中的应用专家共识组. 降钙素原在成人下呼吸道感染性疾病分级管理中的应用专家共识[J]. 中华急诊医学杂志,2021,3(4):393-401.
[6]	祝益民,黄寒. 儿童感染性疾病降钙素原应用专家共识[J]. 实用休克杂志(中英文),2019,3(1):45-51.
[7]	Trevisan C,Noale M,Amidei C B,et al. Frailty and the risk of infection-related hospitalizations in older age: Differences by sex[J]. Maturitas,2023,168:1-6. doi: 10.1016/j.maturitas.2022.10.009
[8]	闫圣涛. 降钙素原对不同细菌感染鉴别诊断和病情评估的临床研究[D]. 北京: 北京协和医学院, 2021.
[9]	Kim S J,Hwang S O,Kim Y W,et al. Procalcitonin as a diagnostic marker for sepsis/septic shock in the emergency department; A study based on sepsis-3 definition[J]. The American Journal of Emergency Medicine,2019,37(2):272-276. doi: 10.1016/j.ajem.2018.05.047
[10]	Yin H,Mo S. Value of combined detection of serum amyloid a,c-reactive protein and procalcitonin in differential diagnosis of respiratory tract infection in children of China[J]. Annals of Medicine,2022,54(1):1732-1737. doi: 10.1080/07853890.2022.2064542
[11]	董晓玉,马晴晴,殷俊,等. 血清IL-6与中性粒细胞表型及吞噬力关系在脓毒症分期中的诊断价值[J]. 安徽医科大学学报,2020,55(8):1265-1270.
[12]	Muna A M,ALhameed R A. The role of C-reactive protein and white blood cell count as diagnostic,prognostic,and monitoring markers in bacterial orofacial infections[J]. Journal of Oral and Maxillofacial Surgery,2022,80(3):530-536. doi: 10.1016/j.joms.2021.08.158
[13]	秦小菀,朱萍,惠晓霞,等. PCT与CRP和WBC及前白蛋白联合检测对儿科感染性疾病早期诊断的价值[J]. 中华医院感染学杂志,2019,29(1):146-148+156.
[14]	吴祖剑. 联合乳酸、C反应蛋白/白蛋白比值的qSOFA评分在腹腔感染中的价值研究[D]. 杭州: 浙江中医药大学, 2022.
[15]	许镜清. 早期乳酸动态监测指标对血流感染相关脓毒性休克患者预后预测价值分析[D]. 福州: 福建医科大学, 2021.
[16]	Dias S P,Brouwer M C,Van de beek d. Sex and gender differences in bacterial infections[J]. Infection and Immunity,2022,90(10):e0028322. doi: 10.1128/iai.00283-22
[17]	Vázquez-Martínez ER,García-gómez E,Camacho-arroyo I,et al. Sexual dimorphism in bacterial infections[J]. Biology of Sex Differences,2018,9(1):1-20. doi: 10.1186/s13293-017-0161-7
[18]	梁涛,杨诚,吴苏源,等. 多项指标联合检测在细菌感染性疾病中的诊断价值[J]. 临床血液学杂志,2019,32(12):905-908,912.
[19]	Gao Q,Li Z,Mo X,et al. Combined procalcitonin and hemogram parameters contribute to early differential diagnosis of gram‐negative/gram‐positive bloodstream infections[J]. Journal of Clinical Laboratory Analysis,2021,35(9):e23927. doi: 10.1002/jcla.23927
[20]	刘海萍,韩蕾,王洪亮,等. 感染指标联合血清蛋白指标在成人下呼吸道鲍曼不动杆菌感染与定植鉴别诊断中的应用价值[J]. 检验医学与临床,2023,20(2):145-149.
[21]	邓勤勤. 联合检测多种炎性指标对呼吸道感染性疾病的价值分析[D]. 广州: 广州中医药大学, 2017.
[22]	Wang Q,Li X,Tang W,et al. Differential gene sets profiling in gram-negative and gram-positive sepsis[J]. Frontiers in Cellular and Infection Microbiology,2022,12:801232. doi: 10.3389/fcimb.2022.801232
[23]	Sun D,Wang Q,Zhang X,et al. Clinical application of serum inflammatory factors combined with dynamic detection in the diagnosis and treatment of neonatal sepsis[J]. Iranian Journal of Public Health,2021,50(2):325-332.
[24]	Vivas M C, Villamarin Guerrero H F, Tascon A J, et al. Plasma interleukin-6 levels correlate with survival in patients with bacterial sepsis and septic shock[J]. Interventional Medicine and Applied Science,2021,11(4):224-230.

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