CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome

Ling WANG; Xiangchuan QIN; Jinqiu LI; Hasim AXIANGU

doi:10.12259/j.issn.2095-610X.S20240103

Volume 45 Issue 1

Jan. 2024

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Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome[J]. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103

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Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome[J]. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103

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CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome

doi: 10.12259/j.issn.2095-610X.S20240103

1.
Basic Medicine College of Xinjiang Medical University/Xinjiang Key Laboratory of Molecular Biology of Endemic Diseases，Urumqi Xinjiang 836001，China

Received Date: 2023-10-30
Available Online: 2024-01-09
Publish Date: 2024-01-25

Abstract

Abstract

Objective To investigate the effect of transmembrane protein CD147 expression on AIM2 inflammasome-mediated pyroptosis and proliferation of cervical cancer cells. Methods Western Blot was used to detect the expression of CD147 in cervical cancer cell lines SiHa（HPV+） and C33a（HPV-） and normal cervical epithelial cells H8（HPV+） and HCer Epic（HPV-）. SiHa cells were transfected with lentivirus to down-regulate the expression of CD147. According to the different treatments, SiHa cells were divided into SiHa group, negative control group（shCD147-NON）, knockdown group 1（shCD147-1） and knockdown group 2（shCD147-2）. The transfection effect was verified by Western Blot, RT-qPCR and green fluorescence expression. The protein and mRNA expressions of AIM2, Caspase-1, IL-18 and GSDMD were detected by Western Blot and RT-qPCR. The lactate dehydrogenase（LDH） release was measured in the cell culture supernatant, and the cell morphology was observed under the fluorescence inverted microscope; the proliferation ability of cells was measured by CCK-8 and the colony formation ability was measured by cell cloning experiments. Results Western Blot results showed that CD147 protein expression in SiHa cells was the highest compared with that in HCerEpic cells. CD147 low expression lentivirus effectively down-regulated the expression of CD147 in SiHa cells. The results of Western Blot and RT-qPCR experiments showed that the expression of AIM 2, Caspase-1, IL-18, GSDMD protein and mRNA increased in shCD147-1 and shCD147-2 group（P < 0.05）. Lactate dehydrogenase（LDH） release assay showed that compared with the SiHa group, the shCD147 group had a significant increase in LDH release（P < 0.05）. Fluorescence inverted microscope showed that the shCD147 group had swelling and vacuolization, showing typical pyroptosis. Compared with the SiHa group, the shCD147-1 and shCD147-2 groups had significantly reduced the cell proliferation and colony formation ability（P < 0.05）. Conclusion Low expression of CD147 effectively up-regulates the expression of AIM2 inflammation-related factors in cervical cancer SiHa cells, induces the pyroptosis, and inhibits the cell proliferation and cloning.
- Cervical cancer,
- CD147,
- AIM2inflammasome,
- Pyroptosis,
- Proliferation

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[1]	Sung H,Ferlay J,Siegel R L,et al. Global cancer statistics 2020: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2021,71(3):209-249. doi: 10.3322/caac.21660
[2]	Kendrick A A,Schafer J,Dzieciatkowska M,et al. Cd147: A small molecule transporter ancillary protein at the crossroad of multiple hallmarks of cancer and metabolic reprogramming[J]. Oncotarget,2017,8(4):6742-6762. doi: 10.18632/oncotarget.14272
[3]	Dana P,Kariya R,Vaeteewoottacharn K,et al. Upregulation of cd147 promotes metastasis of cholangiocarcinoma by modulating the epithelial-to-mesenchymal transitional process[J]. Oncol Res,2017,25(7):1047-1059. doi: 10.3727/096504016X14813899000565
[4]	Schroder K,Tschopp J. The inflammasomes[J]. Cell,2010,140(6):821-832. doi: 10.1016/j.cell.2010.01.040
[5]	Shi J,Gao W,Shao F. Pyroptosis: Gasdermin-mediated programmed necrotic cell death[J]. Trends Biochem Sci,2017,42(4):245-254. doi: 10.1016/j.tibs.2016.10.004
[6]	Rao Z,Zhu Y,Yang P,et al. Pyroptosis in inflammatory diseases and cancer[J]. Theranostics,2022,12(9):4310-4329. doi: 10.7150/thno.71086
[7]	Kumari P,Russo A J,Shivcharan S,et al. Aim2 in health and disease: Inflammasome and beyond[J]. Immunol Rev,2020,297(1):83-95. doi: 10.1111/imr.12903
[8]	Fidler T P,Xue C,Yalcinkaya M,et al. The aim2 inflammasome exacerbates atherosclerosis in clonal haematopoiesis[J]. Nature,2021,592(7853):296-301. doi: 10.1038/s41586-021-03341-5
[9]	郭文涛. Cd147调控srebps影响宫颈癌细胞脂肪酸合成及侵袭迁移能力的实验研究[D]. 乌鲁木齐: 新疆医科大学硕士学位论文, 2020.
[10]	Lee C L,Lam M P,Lam K K,et al. Identification of cd147 (basigin) as a mediator of trophoblast functions[J]. Hum Reprod,2013,28(11):2920-2929. doi: 10.1093/humrep/det355
[11]	Yang H,Chen B. Cd147 in ovarian and other cancers[J]. Int J Gynecol Cancer,2013,23(1):2-8. doi: 10.1097/IGC.0b013e3182749139
[12]	Poli G,Fabi C,Bellet M M,et al. Epigenetic mechanisms of inflammasome regulation[J]. Int J Mol Sci,2020,21(16):5758. doi: 10.3390/ijms21165758
[13]	Wang L,Sun L,Byrd K M,et al. Aim2 inflammasome's first decade of discovery: Focus on oral diseases[J]. Front Immunol,2020,11(8):1487.
[14]	Lee S,Karki R,Wang Y,et al. Aim2 forms a complex with pyrin and zbp1 to drive panoptosis and host defence[J]. Nature,2021,597(7876):415-419. doi: 10.1038/s41586-021-03875-8
[15]	Du T,Gao J,Li P,et al. Pyroptosis,metabolism,and tumor immune microenvironment[J]. Clin Transl Med,2021,11(8):e492. doi: 10.1002/ctm2.492
[16]	Yu P,Zhang X,Liu N,et al. Pyroptosis: Mechanisms and diseases[J]. Signal Transduct Target Ther,2021,6(1):128. doi: 10.1038/s41392-021-00507-5
[17]	Pushkarsky T,Zybarth G,Dubrovsky L,et al. Cd147 facilitates hiv-1 infection by interacting with virus-associated cyclophilin A[J]. Proc Natl Acad Sci USA,2001,98(11):6360-6365. doi: 10.1073/pnas.111583198
[18]	Majno G,Joris I. Apoptosis,oncosis,and necrosis. An overview of cell death[J]. Am J Pathol,1995,146(1):3-15.
[19]	Butera A,Quaranta M T,Crippa L,et al. Cd147 targeting by ac-73 induces autophagy and reduces intestinal fibrosis associated with tnbs chronic colitis[J]. J Crohns Colitis,2022,16(11):1751-1761. doi: 10.1093/ecco-jcc/jjac084
[20]	Kulyar M F,Yao W,Ding Y,et al. Cluster of differentiation 147 (cd147) expression is linked with thiram induced chondrocyte's apoptosis via bcl-2/bax/caspase-3 signalling in tibial growth plate under chlorogenic acid repercussion[J]. Ecotoxicol Environ Saf,2021,213(4):112059.
[21]	曹勋荣,吕桂雪,魏雯雯. Rna干扰抑制cd147表达对宫颈癌细胞增殖、转移和侵袭能力的影响[J]. 中国免疫学杂志,2023,39(2):349-353,358. doi: 10.3969/j.issn.1000-484X.2023.02.023

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