Volume 45 Issue 1
Jan.  2024
Turn off MathJax
Article Contents
Abstract
References
Relative Articles
Article Navigation >  Journal of Kunming Medical University  > 2024  >  45(1): 15-21
Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome[J]. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103
Citation: Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome[J]. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103
shu

CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome

doi: 10.12259/j.issn.2095-610X.S20240103
  • 1.

    Basic Medicine College of Xinjiang Medical University/Xinjiang Key Laboratory of Molecular Biology of Endemic Diseases，Urumqi Xinjiang 836001，China

  • Received Date: 2023-10-30
    Available Online: 2024-01-09
  • Publish Date: 2024-01-25
    Abstract
  •   Objective  To investigate the effect of transmembrane protein CD147 expression on AIM2 inflammasome-mediated pyroptosis and proliferation of cervical cancer cells.  Methods  Western Blot was used to detect the expression of CD147 in cervical cancer cell lines SiHa（HPV+） and C33a（HPV-） and normal cervical epithelial cells H8（HPV+） and HCer Epic（HPV-）. SiHa cells were transfected with lentivirus to down-regulate the expression of CD147. According to the different treatments, SiHa cells were divided into SiHa group, negative control group（shCD147-NON）, knockdown group 1（shCD147-1） and knockdown group 2（shCD147-2）. The transfection effect was verified by Western Blot, RT-qPCR and green fluorescence expression. The protein and mRNA expressions of AIM2, Caspase-1, IL-18 and GSDMD were detected by Western Blot and RT-qPCR. The lactate dehydrogenase（LDH） release was measured in the cell culture supernatant, and the cell morphology was observed under the fluorescence inverted microscope; the proliferation ability of cells was measured by CCK-8 and the colony formation ability was measured by cell cloning experiments.  Results  Western Blot results showed that CD147 protein expression in SiHa cells was the highest compared with that in HCerEpic cells. CD147 low expression lentivirus effectively down-regulated the expression of CD147 in SiHa cells. The results of Western Blot and RT-qPCR experiments showed that the expression of AIM 2, Caspase-1, IL-18, GSDMD protein and mRNA increased in shCD147-1 and shCD147-2 group（P < 0.05）. Lactate dehydrogenase（LDH） release assay showed that compared with the SiHa group, the shCD147 group had a significant increase in LDH release（P < 0.05）. Fluorescence inverted microscope showed that the shCD147 group had swelling and vacuolization, showing typical pyroptosis. Compared with the SiHa group, the shCD147-1 and shCD147-2 groups had significantly reduced the cell proliferation and colony formation ability（P < 0.05）.   Conclusion  Low expression of CD147 effectively up-regulates the expression of AIM2 inflammation-related factors in cervical cancer SiHa cells, induces the pyroptosis, and inhibits the cell proliferation and cloning.
    • FullText(HTML)
    • References(21)
  • [1]
    Sung H,Ferlay J,Siegel R L,et al. Global cancer statistics 2020: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2021,71(3):209-249. doi: 10.3322/caac.21660
    [2]
    Kendrick A A,Schafer J,Dzieciatkowska M,et al. Cd147: A small molecule transporter ancillary protein at the crossroad of multiple hallmarks of cancer and metabolic reprogramming[J]. Oncotarget,2017,8(4):6742-6762. doi: 10.18632/oncotarget.14272
    [3]
    Dana P,Kariya R,Vaeteewoottacharn K,et al. Upregulation of cd147 promotes metastasis of cholangiocarcinoma by modulating the epithelial-to-mesenchymal transitional process[J]. Oncol Res,2017,25(7):1047-1059. doi: 10.3727/096504016X14813899000565
    [4]
    Schroder K,Tschopp J. The inflammasomes[J]. Cell,2010,140(6):821-832. doi: 10.1016/j.cell.2010.01.040
    [5]
    Shi J,Gao W,Shao F. Pyroptosis: Gasdermin-mediated programmed necrotic cell death[J]. Trends Biochem Sci,2017,42(4):245-254. doi: 10.1016/j.tibs.2016.10.004
    [6]
    Rao Z,Zhu Y,Yang P,et al. Pyroptosis in inflammatory diseases and cancer[J]. Theranostics,2022,12(9):4310-4329. doi: 10.7150/thno.71086
    [7]
    Kumari P,Russo A J,Shivcharan S,et al. Aim2 in health and disease: Inflammasome and beyond[J]. Immunol Rev,2020,297(1):83-95. doi: 10.1111/imr.12903
    [8]
    Fidler T P,Xue C,Yalcinkaya M,et al. The aim2 inflammasome exacerbates atherosclerosis in clonal haematopoiesis[J]. Nature,2021,592(7853):296-301. doi: 10.1038/s41586-021-03341-5
    [9]
    郭文涛. Cd147调控srebps影响宫颈癌细胞脂肪酸合成及侵袭迁移能力的实验研究[D]. 乌鲁木齐: 新疆医科大学硕士学位论文, 2020.
    [10]
    Lee C L,Lam M P,Lam K K,et al. Identification of cd147 (basigin) as a mediator of trophoblast functions[J]. Hum Reprod,2013,28(11):2920-2929. doi: 10.1093/humrep/det355
    [11]
    Yang H,Chen B. Cd147 in ovarian and other cancers[J]. Int J Gynecol Cancer,2013,23(1):2-8. doi: 10.1097/IGC.0b013e3182749139
    [12]
    Poli G,Fabi C,Bellet M M,et al. Epigenetic mechanisms of inflammasome regulation[J]. Int J Mol Sci,2020,21(16):5758. doi: 10.3390/ijms21165758
    [13]
    Wang L,Sun L,Byrd K M,et al. Aim2 inflammasome's first decade of discovery: Focus on oral diseases[J]. Front Immunol,2020,11(8):1487.
    [14]
    Lee S,Karki R,Wang Y,et al. Aim2 forms a complex with pyrin and zbp1 to drive panoptosis and host defence[J]. Nature,2021,597(7876):415-419. doi: 10.1038/s41586-021-03875-8
    [15]
    Du T,Gao J,Li P,et al. Pyroptosis,metabolism,and tumor immune microenvironment[J]. Clin Transl Med,2021,11(8):e492. doi: 10.1002/ctm2.492
    [16]
    Yu P,Zhang X,Liu N,et al. Pyroptosis: Mechanisms and diseases[J]. Signal Transduct Target Ther,2021,6(1):128. doi: 10.1038/s41392-021-00507-5
    [17]
    Pushkarsky T,Zybarth G,Dubrovsky L,et al. Cd147 facilitates hiv-1 infection by interacting with virus-associated cyclophilin A[J]. Proc Natl Acad Sci USA,2001,98(11):6360-6365. doi: 10.1073/pnas.111583198
    [18]
    Majno G,Joris I. Apoptosis,oncosis,and necrosis. An overview of cell death[J]. Am J Pathol,1995,146(1):3-15.
    [19]
    Butera A,Quaranta M T,Crippa L,et al. Cd147 targeting by ac-73 induces autophagy and reduces intestinal fibrosis associated with tnbs chronic colitis[J]. J Crohns Colitis,2022,16(11):1751-1761. doi: 10.1093/ecco-jcc/jjac084
    [20]
    Kulyar M F,Yao W,Ding Y,et al. Cluster of differentiation 147 (cd147) expression is linked with thiram induced chondrocyte's apoptosis via bcl-2/bax/caspase-3 signalling in tibial growth plate under chlorogenic acid repercussion[J]. Ecotoxicol Environ Saf,2021,213(4):112059.
    [21]
    曹勋荣,吕桂雪,魏雯雯. Rna干扰抑制cd147表达对宫颈癌细胞增殖、转移和侵袭能力的影响[J]. 中国免疫学杂志,2023,39(2):349-353,358. doi: 10.3969/j.issn.1000-484X.2023.02.023
    • Relative Articles(20)

  • Relative Articles

    [1] Kun ZHOU, Yali LIU, Ziliang LI, Liping QIAN, Liquan RAN, Yalan REN. Effect of miR-34a on Proliferation and Osteogenic Differentiation of Human Periodontal Stem Cells. Journal of Kunming Medical University, 2025, 46(): 1-7.
    [2] Yiran YAN, Chengwan SHEN, Xiangyu SHANG, Chan FENG, Jinqiu LI, Hasim AXIANGU. Galangin Inhibits the Migration and Invasion of Cervical Cancer Hela Cells Through Hippo/YAP Pathway. Journal of Kunming Medical University, 2025, 46(1): 36-42.  doi: 10.12259/j.issn.2095-610X.S20250105
    [3] Chunxia MIAO, Yina WANG, Yunxia ZHANG. Expression of EMC6，C-myc and BCL-2 in Cervical Cancer Tissues and Prognosis Analysis. Journal of Kunming Medical University, 2024, 45(7): 62-68.  doi: 10.12259/j.issn.2095-610X.S20240709
    [4] Yuru ZHAI, Yan BAI, Yunyun LI. FGF2 Regulates Hypoxia-Induced Proliferation and Collagen Metabolism of Scleral Fibroblasts Through the PERK/EIF2α/ATF4 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(10): 22-28.  doi: 10.12259/j.issn.2095-610X.S20241004
    [5] Xiangchuan QIN, Jinqiu LI, Xiaojing HUANG, Kuerban HUTUBIDING·, Hasim AXIANGU·. Effect of HPV E6 on Proliferation，Invasion and Migration of Cervical Cancer Cells Through Rap1 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(9): 9-16.  doi: 10.12259/j.issn.2095-610X.S20240902
    [6] Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells. Journal of Kunming Medical University, 2023, 44(10): 47-54.  doi: 10.12259/j.issn.2095-610X.S20231028
    [7] Jing WANG, Hongying MI, Yi ZHANG, Li LI, Jianhua YU, Liqiao LIU, Qingyu LIU, Liwei WANG. Pyrotosis Participates in the Pathogenesis of Necrotizing Enterocolitis in Early Offspring Rats. Journal of Kunming Medical University, 2023, 44(1): 25-30.  doi: 10.12259/j.issn.2095-610X.S20230124
    [8] Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells. Journal of Kunming Medical University, 2023, 44(9): 7-14.  doi: 10.12259/j.issn.2095-610X.S20230926
    [9] Yan HAI, Tursun MIKRBAN, Hasim AXIANGU. Effects of Pin1 on the Proliferation and Apoptosis of Cervical Cancer SiHa Cells by Regulating Key Enzymes of Lipid Metabolism. Journal of Kunming Medical University, 2023, 44(1): 1-6.  doi: 10.12259/j.issn.2095-610X.S20230107
    [10] Bin ZHAO, Yuanpeng DUAN, Guoying ZHANG, Chengwei BI, Libo YANG, Zhiyu SHI, Yong YANG, Jianpeng ZHANG, Ting GAO. CircRNA EZH2 Promotes Proliferation and Migration of Prostate Cancer Cells by Regulating miR-30c-5p. Journal of Kunming Medical University, 2022, 43(7): 25-32.  doi: 10.12259/j.issn.2095-610X.S20220731
    [11] Wei ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Liang ZHANG. miR-125b-5p Regulates HK2 to Inhibit Proliferation and Glycolysis of Gallbladder Cancer Cells. Journal of Kunming Medical University, 2022, 43(12): 23-29.  doi: 10.12259/j.issn.2095-610X.S20221206
    [12] Xi-cui LONG, Bei-bei LIU, Shao-bo LU, Zhi-hong LI, Wen-jiao JIN, Jin-zhi LU, Xue-song HAN. The Expression and Significance of Pyroptosis Factor Cytokine Caspase-1、IL-1β and IL-18 in Endometrial Polys. Journal of Kunming Medical University, 2021, 42(9): 51-56.  doi: 10.12259/j.issn.2095-610X.S20210917
    [13] Yang LIU, Jing LIAO, You-hui LU, Chun-yi SUN, Yu-shi MENG. Negative Regulation of hTERT Expression in Cervical Cancer Hela Cell Line by miR-21. Journal of Kunming Medical University, 2021, 42(3): 23-28.  doi: 10.12259/j.issn.2095-610X.S20210307
    [14] Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells. Journal of Kunming Medical University, 2021, 42(5): 18-23.  doi: 10.12259/j.issn.2095-610X.S20210504
    [15] Xiao-ying WANG, Zuo-jin LIU, Li-juan SHEN. Review of Correlation between Ischemia-reperfusion Injury and Pyroptosis. Journal of Kunming Medical University, 2020, 41(12): 142-147.  doi: 10.12259/j.issn.2095-610X.S20201240
    [16] Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
    [17] Wei Dong . Effect of Fragile Site WWOX Gene on Regulating Proliferation of Human Gallbladder Cancer Cells in Vitro. Journal of Kunming Medical University,
    [18] Zhu Sheng Zhang . Clinical Analysis of Liquid-based Cervical Cytology Examination in Cervical Cancer Screening in the South of GuiZhou. Journal of Kunming Medical University,
    [19] Yang Zheng Hao . . Journal of Kunming Medical University,
    [20] Wang Hai Feng . . Journal of Kunming Medical University,
    • Supplements(0)
    • Cited By

  • Cited by

    Periodical cited type(8)

    1. 郑一，刘继伟，于喜红，何燕斐，王建军，杨瑞. 不同表型COPD患者HRCT参数与T淋巴细胞亚群水平、肺功能参数的相关性. 分子诊断与治疗杂志. 2023(01): 43-47 .
    2. 白金录，刘照龙，杨丽娜，卞海艳，李侠. 血清SERPINA1、CTGF、T-AOC在子宫肌瘤患者中的表达水平及临床意义. 河北医科大学学报. 2023(04): 434-438 .
    3. 葛进男，张晓风，葛蕾蕾，许晓霞. 早期肺康复对慢性阻塞性肺疾病急性加重期患者的影响. 当代护士(中旬刊). 2023(03): 27-30 .
    4. 宋丽英. 慢性阻塞性肺疾病急性加重的影响因素分析及预测模型的构建与验证. 南昌大学学报(医学版). 2023(06): 67-71 .
    5. 蒲小娟，彭芳，赵启全，陈远婷，刘梅婷. 多索茶碱联合噻托溴铵对COPD患者血清APN、NE及α1-AT水平的影响. 保健医学研究与实践. 2023(10): 38-42 .
    6. 鄞晓斌，邱名耀，吴海琴，林明霞，林芳崇. 血清α1-AT和NE水平变化与AECOPD患者病情程度关系及预测呼吸衰竭的价值. 临床急诊杂志. 2022(06): 390-394 .
    7. 卢亚茹. 血嗜酸性粒细胞与慢性阻塞性肺疾病对糖皮质激素治疗反应的关系. 慢性病学杂志. 2022(09): 1418-1420 .
    8. 李琼，张庭秀，胡绳，周宏. 支气管扩张患者TNF-α、IL-6、中性粒细胞百分比与疾病严重程度的关系. 中华肺部疾病杂志(电子版). 2022(05): 649-652 .

    Other cited types(2)

  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-040255075100
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 36.2 %FULLTEXT: 36.2 %META: 62.9 %META: 62.9 %PDF: 0.9 %PDF: 0.9 %FULLTEXTMETAPDF
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 5.0 %其他: 5.0 %其他: 0.2 %其他: 0.2 %Bang Khlo: 0.2 %Bang Khlo: 0.2 %China: 0.3 %China: 0.3 %Falkenstein: 0.1 %Falkenstein: 0.1 %Wixom: 0.1 %Wixom: 0.1 %上海: 9.6 %上海: 9.6 %东莞: 0.1 %东莞: 0.1 %乌鲁木齐: 0.4 %乌鲁木齐: 0.4 %伊犁: 0.1 %伊犁: 0.1 %保定: 0.1 %保定: 0.1 %保山: 0.1 %保山: 0.1 %六安: 0.1 %六安: 0.1 %六盘水: 0.2 %六盘水: 0.2 %北京: 32.9 %北京: 32.9 %十堰: 0.1 %十堰: 0.1 %南京: 0.4 %南京: 0.4 %南宁: 0.3 %南宁: 0.3 %南通: 0.2 %南通: 0.2 %博阿努瓦: 0.1 %博阿努瓦: 0.1 %合肥: 0.1 %合肥: 0.1 %吉林: 0.1 %吉林: 0.1 %呼和浩特: 0.1 %呼和浩特: 0.1 %咸阳: 0.1 %咸阳: 0.1 %哈尔滨: 0.1 %哈尔滨: 0.1 %哥伦布: 0.1 %哥伦布: 0.1 %唐山: 0.1 %唐山: 0.1 %天津: 0.4 %天津: 0.4 %太原: 0.1 %太原: 0.1 %安那罕: 1.3 %安那罕: 1.3 %宣城: 0.2 %宣城: 0.2 %张家口: 3.6 %张家口: 3.6 %徐州: 0.1 %徐州: 0.1 %成都: 0.3 %成都: 0.3 %扬州: 0.3 %扬州: 0.3 %承德: 0.1 %承德: 0.1 %无锡: 0.3 %无锡: 0.3 %昆明: 1.8 %昆明: 1.8 %晋城: 0.1 %晋城: 0.1 %曲靖: 0.1 %曲靖: 0.1 %朝阳: 0.1 %朝阳: 0.1 %杭州: 0.2 %杭州: 0.2 %柳州: 0.1 %柳州: 0.1 %格兰特县: 0.1 %格兰特县: 0.1 %榆林: 0.1 %榆林: 0.1 %武汉: 0.2 %武汉: 0.2 %沈阳: 0.1 %沈阳: 0.1 %泰州: 0.1 %泰州: 0.1 %洛阳: 0.2 %洛阳: 0.2 %浦那: 0.2 %浦那: 0.2 %淄博: 0.1 %淄博: 0.1 %深圳: 0.5 %深圳: 0.5 %温州: 0.1 %温州: 0.1 %湖州: 0.1 %湖州: 0.1 %漯河: 1.3 %漯河: 1.3 %濮阳: 0.1 %濮阳: 0.1 %白山: 0.2 %白山: 0.2 %眉山: 0.1 %眉山: 0.1 %石家庄: 0.3 %石家庄: 0.3 %绍兴: 0.1 %绍兴: 0.1 %绵阳: 0.1 %绵阳: 0.1 %芒廷维尤: 10.0 %芒廷维尤: 10.0 %芝加哥: 0.3 %芝加哥: 0.3 %苏州: 0.3 %苏州: 0.3 %衡阳: 0.1 %衡阳: 0.1 %衢州: 0.2 %衢州: 0.2 %西宁: 8.8 %西宁: 8.8 %西安: 0.1 %西安: 0.1 %贵阳: 0.2 %贵阳: 0.2 %赤峰: 0.1 %赤峰: 0.1 %运城: 0.2 %运城: 0.2 %邯郸: 0.3 %邯郸: 0.3 %郑州: 0.4 %郑州: 0.4 %重庆: 0.1 %重庆: 0.1 %银川: 0.2 %银川: 0.2 %长沙: 0.7 %长沙: 0.7 %青岛: 0.3 %青岛: 0.3 %马赛: 0.2 %马赛: 0.2 %驻马店: 15.1 %驻马店: 15.1 %其他其他Bang KhloChinaFalkensteinWixom上海东莞乌鲁木齐伊犁保定保山六安六盘水北京十堰南京南宁南通博阿努瓦合肥吉林呼和浩特咸阳哈尔滨哥伦布唐山天津太原安那罕宣城张家口徐州成都扬州承德无锡昆明晋城曲靖朝阳杭州柳州格兰特县榆林武汉沈阳泰州洛阳浦那淄博深圳温州湖州漯河濮阳白山眉山石家庄绍兴绵阳芒廷维尤芝加哥苏州衡阳衢州西宁西安贵阳赤峰运城邯郸郑州重庆银川长沙青岛马赛驻马店

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(4)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (1209) PDF downloads(16) Cited by(10)
    Proportional views
    Related

    Copyright © 《昆明医科大学学报》编辑部  滇ICP备00000000号-0

    Publishing: 昆明医科大学学报编辑部

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return