Comparison of Methods for Generating Thrombotic Models after the Endothelial Injury

Jingqiu ZHANG; Yongping LU; Linkun TA; Yue SUN; Qi CHEN

doi:10.12259/j.issn.2095-610X.S20240112

Volume 45 Issue 1

Jan. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(1): 35-40

Jingqiu ZHANG, Yongping LU, Linkun TA, Yue SUN, Qi CHEN. Comparison of Methods for Generating Thrombotic Models after the Endothelial Injury[J]. Journal of Kunming Medical University, 2024, 45(1): 35-40. doi: 10.12259/j.issn.2095-610X.S20240112

Citation:

Jingqiu ZHANG, Yongping LU, Linkun TA, Yue SUN, Qi CHEN. Comparison of Methods for Generating Thrombotic Models after the Endothelial Injury[J]. Journal of Kunming Medical University, 2024, 45(1): 35-40. doi: 10.12259/j.issn.2095-610X.S20240112

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Comparison of Methods for Generating Thrombotic Models after the Endothelial Injury

doi: 10.12259/j.issn.2095-610X.S20240112

Dept. of Medical Ultrasound，The Affiliated Hospital of Yunnan University/ The 2nd People's Hospital of Yunnan Province，Kunming Yunnan 650021，China

Received Date: 2023-09-25
Publish Date: 2024-01-25

Abstract

Abstract

Objective Endothelial injury plays a crucial role in forming deep vein thrombosis. This study aims to compare the effectiveness of various methods for creating rabbit femoral vein thrombotic models after the endothelial injuryso as to provide a solid experimental foundation for further research on the endothelial injury and deep vein thrombosis. Methods Forty-five rabbits were randomly divided into three groups（A, B, C）, with 15 cases in each group and subjected to the endothelial injury using the methods of simple clamping, combined complete ligation, and combined incomplete ligation, respectively. The intravascular ultrasonic manifestations and local endothelial pathological changes were compared at 1, 3, and 7 days after modeling. Results Significant differences in vascular diameter and Young’ s modulus values were observed after 7 days of modeling（P < 0.05）. In pairwise comparisons between the groups, the Young’ s modulus values in group C were significantly higher than those in groups A and B after 7 days of modeling（ P < 0.05）. Pathological examination confirmed the presence of fibrinoid thrombus in the blood vessels of group C on the seventh day of modeling. Conclusion Combining simple clamping and incomplete ligation can produce a relatively stable endothelial injury and thrombus formation. This method provides a robust experimental model for further investigation into deep vein thrombosis after the endothelial injury.
- Rabbit,
- Endothelial injury,
- Venous thrombosis,
- Real-time shear wave elastography

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References(15)

References

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