The Effects of Bronchoalveolar Lavage Combined with Microbiological Rapid on-site Evaluation in the Maintenance of Potential Donor Lung

Liming GONG; Jianghua RAN; Yinjia WANG; Zhiwei LI; Qian YANG; Qing WANG; Dongkun WANG; Zhengneng TANG

doi:10.12259/j.issn.2095-610X.S20240118

Volume 45 Issue 1

Jan. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(1): 107-115

Liming GONG, Jianghua RAN, Yinjia WANG, Zhiwei LI, Qian YANG, Qing WANG, Dongkun WANG, Zhengneng TANG. The Effects of Bronchoalveolar Lavage Combined with Microbiological Rapid on-site Evaluation in the Maintenance of Potential Donor Lung[J]. Journal of Kunming Medical University, 2024, 45(1): 107-115. doi: 10.12259/j.issn.2095-610X.S20240118

Citation:

Liming GONG, Jianghua RAN, Yinjia WANG, Zhiwei LI, Qian YANG, Qing WANG, Dongkun WANG, Zhengneng TANG. The Effects of Bronchoalveolar Lavage Combined with Microbiological Rapid on-site Evaluation in the Maintenance of Potential Donor Lung[J]. Journal of Kunming Medical University, 2024, 45(1): 107-115. doi: 10.12259/j.issn.2095-610X.S20240118

Citation:

Liming GONG, Jianghua RAN, Yinjia WANG, Zhiwei LI, Qian YANG, Qing WANG, Dongkun WANG, Zhengneng TANG. The Effects of Bronchoalveolar Lavage Combined with Microbiological Rapid on-site Evaluation in the Maintenance of Potential Donor Lung[J]. Journal of Kunming Medical University, 2024, 45(1): 107-115. doi: 10.12259/j.issn.2095-610X.S20240118

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The Effects of Bronchoalveolar Lavage Combined with Microbiological Rapid on-site Evaluation in the Maintenance of Potential Donor Lung

doi: 10.12259/j.issn.2095-610X.S20240118

1.
Dept. of Cardiothoracic Surgery
2.
Dept. of Hepatobiliary Surgery
3.
Dept of Intensive Care Unit
4.
Dept. of Pathology
5.
Dept. of Respiratory and Critical Care Medicine，The 1st People’s Hospital of Kunming，The Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650011，China

Received Date: 2023-09-20
Available Online: 2024-01-09
Publish Date: 2024-01-25

Abstract

Abstract

Objective To explore the effects of bronchoalveolar lavage combined with microbiological rapid on-site evaluation in potential donor lung maintenance. Methods Brain death patients who met the inclusion criteria and were admitted to the Intensive Care Unit（ICU） of Calmette Hospital Affiliated to Kunming Medical University from September 2020 to December 2022 were selected for bronchoalveolar lavage（BAL） and（BAL） and the lavage fluid were collected for M-ROSE to compare the pathogen detection rate and initial diagnosis time. According to the positive results of the microbiological rapid on-site evaluation, patients with the brain death were treated with empirical anti-infective therapy, and the oxygenation index, chest X-ray score, and the infection index（WBC, CRP, PCT） of anti-infective treatment 48 hours were evaluated. Results 1.Comparison of the detection rate of pathogenic microorganisms: The results of M-ROSE were highly consistent with a routine microbiological smear（Kappa = 0.921, P < 0.001）. 2.Comparison of diagnostic time: The initial diagnosis time of M-ROSE was significantly lower than routine microbiological smear time and microbial culture time（P < 0.001）. 3.Comparison of therapeutic effects of anti-infective therapy for 48 hours: There was no significant difference in oxygenation index, white blood cells and hypersensitive C-reactive protein before and after the anti-infective treatment（P > 0.05）. There were significant differences in procalcitonin and chest X-ray before and after the anti-infective treatment（P < 0.05）. Conclusion Bronchoalveolar lavage combined with microbiological rapid on-site evaluation has the high timeliness in the diagnosis of potential donor pulmonary infection, which can provide a preliminary basis for the early anti-infective therapy of donor lung maintenance.
- Lung transplantation,
- Brain death,
- Donor lung maintenance,
- Bronchoalveolar lavage,
- Microbiological rapid on-site evaluation.

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