Role of HK2 and VDAC1 in Diacetylmorphine-induced Cardiomyocyte Apoptosis

Jinling XIAO; Yaling GUAN; Sensen ZHU; Mengjie ZHUANG; Liping SU; Hongwei PU

doi:10.12259/j.issn.2095-610X.S20240202

Volume 45 Issue 2

Feb. 2024

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Jinling XIAO, Yaling GUAN, Sensen ZHU, Mengjie ZHUANG, Liping SU, Hongwei PU. Role of HK2 and VDAC1 in Diacetylmorphine-induced Cardiomyocyte Apoptosis[J]. Journal of Kunming Medical University, 2024, 45(2): 7-13. doi: 10.12259/j.issn.2095-610X.S20240202

Citation:

Jinling XIAO, Yaling GUAN, Sensen ZHU, Mengjie ZHUANG, Liping SU, Hongwei PU. Role of HK2 and VDAC1 in Diacetylmorphine-induced Cardiomyocyte Apoptosis[J]. Journal of Kunming Medical University, 2024, 45(2): 7-13. doi: 10.12259/j.issn.2095-610X.S20240202

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Role of HK2 and VDAC1 in Diacetylmorphine-induced Cardiomyocyte Apoptosis

doi: 10.12259/j.issn.2095-610X.S20240202

1.
Dept. of Pathology，School of Basic Medical Sciences，Xinjiang Medical University，Urumqi，Xinjiang Uygur Autonomous Region Xinjiang 830011
2.
Dept. of Pathology，The 1st Affiliated Hospital of Xinjiang Medical University，Urumqi Xinjiang Uygur Autonomous Region 830011
3.
Dept. of Discipline Construction，Urumqi Xinjiang Uygur Autonomous Region 830011，China

Received Date: 2023-11-29
Available Online: 2024-02-27
Publish Date: 2024-02-25

Abstract

Abstract

Objective To investigate the role of HK2 and VDAC1 in diacetylmorphine-induced cardiomyocyte apoptosis. Methods A dose-escalation method was used to establish a rat model of diacetylmorphine addiction. Forty SD rats were randomly divided into three groups, the normal group （n=10） was injected with an equal amount of saline subcutaneously, the model group （n=15） was injected with 5 mg/kg of diacetylmorphine for the first time, and then the dose was increased by 2.5 mg/ （kg·d） day by day for 20 days, and the group of model + 10 D （n=15） continued to increase the dose based on the model group up to the 10th day. Lactate dehydrogenase （LDH） and glutamic oxaloacetic transaminase （GOT） were detected by ELISA; HE staining was used to observe the pathological changes of myocardial tissues in each group; TUNEL staining was used to detect apoptosis in myocardial tissues in each group; and immunohistochemistry, RT-q-analysis, and immunochemistry were used to detect apoptosis in myocardial tissues in each group. Immunohistochemistry, RT-qPCR and Western blot were used to detect the mRNA and protein expression of HK2, VDAC1 and apoptosis-related factors. Results HE staining revealed that myocardial tissues exhibited different degrees of damage with the prolongation of diacetylmorphine intervention. Compared with the normal group, serum LDH, GOT content and myocardial apoptosis rate increased in the model group, mRNA and protein levels of HK2 and anti-apoptotic factor Bcl-2 decreased, mRNA and protein levels of VDAC1 and pro-apoptotic factors Bax and Caspase-3 increased, and the protein level of Clevead Caspase-3 increased; in the model + 10 D group the above indexes, there was a statistically significant difference （P < 0.05）. Conclusion Diacetylmorphine can cause cardiomyocyte apoptosis, and VDAC1 may be involved in the process of cardiomyocyte apoptosis caused by diacetylmorphine.
- Diacetylmorphine,
- Cardiac apoptosis,
- HK2,
- VDAC1,
- Clevead Caspase-3

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References(24)

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