Study on the Relationship Between OAT1 Expression and Renal Osteodystrophy in Rats with Chronic Renal Failure

Wenjuan SHEN; Wenxin LIANG; Qing LI; Hongfang XU; weiqin ZHANG; Haixin LI

doi:10.12259/j.issn.2095-610X.S20240205

Volume 45 Issue 2

Feb. 2024

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Wenjuan SHEN, Wenxin LIANG, Qing LI, Hongfang XU, weiqin ZHANG, Haixin LI. Study on the Relationship Between OAT1 Expression and Renal Osteodystrophy in Rats with Chronic Renal Failure[J]. Journal of Kunming Medical University, 2024, 45(2): 32-38. doi: 10.12259/j.issn.2095-610X.S20240205

Citation:

Wenjuan SHEN, Wenxin LIANG, Qing LI, Hongfang XU, weiqin ZHANG, Haixin LI. Study on the Relationship Between OAT1 Expression and Renal Osteodystrophy in Rats with Chronic Renal Failure[J]. Journal of Kunming Medical University, 2024, 45(2): 32-38. doi: 10.12259/j.issn.2095-610X.S20240205

Citation:

Wenjuan SHEN, Wenxin LIANG, Qing LI, Hongfang XU, weiqin ZHANG, Haixin LI. Study on the Relationship Between OAT1 Expression and Renal Osteodystrophy in Rats with Chronic Renal Failure[J]. Journal of Kunming Medical University, 2024, 45(2): 32-38. doi: 10.12259/j.issn.2095-610X.S20240205

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Study on the Relationship Between OAT1 Expression and Renal Osteodystrophy in Rats with Chronic Renal Failure

doi: 10.12259/j.issn.2095-610X.S20240205

1.
Pathology and Physiology Teaching and Research Office of Haiyuan College，Kunming Medical University
2.
Dept. of Human Anatomy
3.
Medical Functionality Laboratory
4.
Human Parasitology Teaching and Research Office，Kunming Yunnan 651700
5.
Dept. of Orthopaedics，Kunming 2nd People’s Hospital，Kunming Yunnan 650204，China

Received Date: 2024-01-20
Available Online: 2024-02-27
Publish Date: 2024-02-25

Abstract

Abstract

Objective To investigate the changes of OAT expression in bone cells in chronic renal failure （CRF）and involved mechanism, and to explore the effect of OAT expression on bone metabolism. Methods Randomly divide the rats into a control group （n=6）and a model group （n=6）.The model group established a rat chronic renal failure model using "single nephrectomy+adenine gavage" method, and the red blood cell （RBC） and hemoglobin （HGB）of the rat body were measured using a blood routine analyzer; Measure indicators such as creatinine （Cr）, urea nitrogen （BUN）, uric acid （UA）, blood calcium （Ca²⁺）, and blood phosphorus （P³⁺） using a fully automated biochemical analyzer; Pathological examination of rat kidneys; X-ray examination of rat tibia; Immunohistochemical examination of bone tissue OAT1 level. Results The bone density of the model group rats is lower than that of the control group;The calcium and phosphorus metabolism of rats in the model group was in metabolic disorder, and the OAT1 value of bone tissue binding was much lower than that of the control group（P=0.0018）, which was statistically significant. （P=0.0018） Conclusion Chronic renal failure affected the binding ability of OAT1 in bone tissue, leading to the metabolic disorder for calcium absorption and phosphorus metabolism, thus aggravating renal osteodystrophy （P<0.05）.
- Organic anion transporter,
- Chronic renal failure,
- Osteodystrophy

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