Effect of MSC-exo，a New Cell Delivery Tool，on Gene Delivery and Proliferation of Pancreatic Cancer

Lei ZHU; Ruixue LI; Changlei BAO; Chenchen HUANG; Shuxin LIANG; Zhenlin ZHAO; Hong ZHU

doi:10.12259/j.issn.2095-610X.S20240206

Volume 45 Issue 2

Feb. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(2): 39-48

Lei ZHU, Ruixue LI, Changlei BAO, Chenchen HUANG, Shuxin LIANG, Zhenlin ZHAO, Hong ZHU. Effect of MSC-exo，a New Cell Delivery Tool，on Gene Delivery and Proliferation of Pancreatic Cancer[J]. Journal of Kunming Medical University, 2024, 45(2): 39-48. doi: 10.12259/j.issn.2095-610X.S20240206

Citation:

Lei ZHU, Ruixue LI, Changlei BAO, Chenchen HUANG, Shuxin LIANG, Zhenlin ZHAO, Hong ZHU. Effect of MSC-exo，a New Cell Delivery Tool，on Gene Delivery and Proliferation of Pancreatic Cancer[J]. Journal of Kunming Medical University, 2024, 45(2): 39-48. doi: 10.12259/j.issn.2095-610X.S20240206

Citation:

Lei ZHU, Ruixue LI, Changlei BAO, Chenchen HUANG, Shuxin LIANG, Zhenlin ZHAO, Hong ZHU. Effect of MSC-exo，a New Cell Delivery Tool，on Gene Delivery and Proliferation of Pancreatic Cancer[J]. Journal of Kunming Medical University, 2024, 45(2): 39-48. doi: 10.12259/j.issn.2095-610X.S20240206

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Effect of MSC-exo，a New Cell Delivery Tool，on Gene Delivery and Proliferation of Pancreatic Cancer

doi: 10.12259/j.issn.2095-610X.S20240206

1.
Shenzhen Ripson Stem Cell and Regenerative Medicine Research Institute，Guangzhou Shenzhen 518038，China

Received Date: 2023-11-01
Available Online: 2024-02-26
Publish Date: 2024-02-25

Abstract

Abstract

Objective To observe the effect of a new cell delivery tool （MSC exo） on the proliferation of pancreatic cancer by transferring targeted genes. Methods Transmission Electron Microscope （TEM） and Nanoparticle Tracking Analysis（NTA） were used to identify human mesenchymal stem cell exosomes（MSC-exo） and transport miR-450a-5p into CFPAC-1, to explore the effect of miR-450a-5p targeting BZW2 on inhibiting the proliferation of pancreatic cancer cells. Results The expression of miR-450a-5p was low in pancreatic cancer tissue （P<0.05）, and the expression of CD63 and TSG101 of MSC-exo-miR-450a-5p in CFPAC-1 cells was higher than that of MSC-exo by Western blot（P<0.05）. CCK-8 and EdU results showed that MSC-exo-miR-450a-5p significantly inhibited the proliferation of CFPAC-1 cells （P<0.05）. Cell scratch and Transwell experiments showed that MSC-exo-miR-450a-5p can inhibit the migration and invasion of CFPAC-1 cells （P<0.05）. Through dual luciferase assay, it was confirmed that miR-450a-5p targets BZW2, and RT-qPCR and Western blotting showed a negative correlation （P<0.05） between miR-450a-5p and BZW2 expression. Overexpression of BZW2, CCK-8, EdU, cell scratch, and Transwell experiments confirmed that pc-BZW2 reversed the anti-cancer function of MSC-exo-miR-450a-5p on CFPAC-1. Western blot detected PCNA,Ki-67,MMP2,MMP9, and the results were consistent with the above experiments （P<0.05）. Conclusion hMSC exo is a new delivery system, targeting BZW2 to transport miR-450a-5p to inhibit the biological malignancy of pancreatic cancer cells, which provides an important clue for the research of targeted treatment of pancreatic cancer.
- Pancreatic adenocarcinoma,
- Mesenchymal stem cell,
- Exosomes,
- miR-450a-5p,
- BZW2

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