Zhi YAN, Jingang HAO, Yunyun SHANG. Value of VI-RADS Scoring System in Precision Treatment of Bladder Cancer[J]. Journal of Kunming Medical University, 2022, 43(3): 74-79. doi: 10.12259/j.issn.2095-610X.S20220322
Citation: Maimaitizunong Rezeye, Xiujuan LI, Ling LIU, Hui LI. Effects of Ferroptosis Inhibitor KIF20A on Biological Behavior and Ferroptosis of Esophageal Carcinoma Cells[J]. Journal of Kunming Medical University, 2024, 45(2): 49-56. doi: 10.12259/j.issn.2095-610X.S20240207

Effects of Ferroptosis Inhibitor KIF20A on Biological Behavior and Ferroptosis of Esophageal Carcinoma Cells

doi: 10.12259/j.issn.2095-610X.S20240207
  • Received Date: 2023-11-09
    Available Online: 2024-02-27
  • Publish Date: 2024-02-25
  •   Objective  To investigate the expression of KIF20A in ESCC and its effect on Eca109 cell biological behavior and ferroptosis.   Methods  The expression of KIF20A in ESCC was predicted by bioinformatics analysis. KIF20A knockdown /overexpression Eca109 cell line was constructed, Experimental groups: control group, KIF20A knockdown group, KIF20A overexpression group, and the effects of KIF20A on the proliferation, invasion, and migration of Eca109 cells were detected by CCK-8, Transwell invasion assay and cell scratch assay. On this basis, the ferroptosis model induced by RSL3 was established, and GSH and MDA contents were detected.   Results  Compared with the control group, the cell proliferation activity of the KIF20A knockdown group was significantly decreased (P < 0.05), and that of the KIF20A overexpression group was significantly increased (P < 0.05). Compared with the control group, the invasion and migration ability of the KIF20A knockdown group was decreased (P < 0.05), and the invasion and migration ability of the KIF20A overexpression group was increased (P < 0.05). After RSL3 induction, the GSH content in the cell lysate of the KIF20A knockdown group was lower than that of the control group (P < 0.05), and the MDA content was higher than that of the control group (P < 0.05).   Conclusion  KIF20A is highly expressed in ESCC and plays a role in promoting proliferation, invasion, migration and inhibiting ferroptosis in Eca109 cells.
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