Comparison of Four Methods in the Diagnosis of Bone Tuberculosis

Xiaoqin LI; Zhiqiang MA; Nan TIAN; Wei CHEN; Caixin LI; Min ZHOU

doi:10.12259/j.issn.2095-610X.S20240218

Volume 45 Issue 2

Feb. 2024

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Xiaoqin LI, Zhiqiang MA, Nan TIAN, Wei CHEN, Caixin LI, Min ZHOU. Comparison of Four Methods in the Diagnosis of Bone Tuberculosis[J]. Journal of Kunming Medical University, 2024, 45(2): 131-135. doi: 10.12259/j.issn.2095-610X.S20240218

Citation:

Xiaoqin LI, Zhiqiang MA, Nan TIAN, Wei CHEN, Caixin LI, Min ZHOU. Comparison of Four Methods in the Diagnosis of Bone Tuberculosis[J]. Journal of Kunming Medical University, 2024, 45(2): 131-135. doi: 10.12259/j.issn.2095-610X.S20240218

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Comparison of Four Methods in the Diagnosis of Bone Tuberculosis

doi: 10.12259/j.issn.2095-610X.S20240218

Xiaoqin LI¹,
Zhiqiang MA¹,
Nan TIAN²,
Wei CHEN¹,
Caixin LI^{1
,
,},
Min ZHOU^{1
,
,}

1.
Dept. of Laboratory Infectious Disease，The Third People’s Hospital of Kunming，Yunnan Clinical Medical Center for Infectious Diseases，Kunming Yunnan 650031
2.
School of Public Health，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2023-12-19
Available Online: 2024-02-26
Publish Date: 2024-02-25

Abstract

Abstract

Objective To compare the diagnostic value of smear acid-fast staining, TB-DNA, X-pert MTB/RIF and culture of Mycobacterium tuberculosis. Methods Four methods were used to detect the perifocal pus of the patients with bone destruction in orthopaedics department within one year, and the results were analyzed statistically, the indexes included sensitivity, specificity, positive predictive value and negative predictive value. Results The sensitivity, specificity, positive predictive value, negative predictive value, and Youden index were 31.75%, 100.00%, 100.00%, 53.74% and 0.32 respectively. TB-DNA had a sensitivity of 88.89%, a specificity of 98.00%, a positive predictive value of 98.25%, a negative predictive value of 87.50%, and a Youden Index of 0.87. Xpert MTB/Rif had a sensitivity of 95.23%, a specificity of 68.00%, and a positive predictive value of 78.95%, the negative predictive value was 91.90%, the Youden index was 0.63. The sensitivity, the specificity, the positive predictive value, the negative predictive value and the Youden index were 41.27%, 100.00%, 100.00%, 57.47% and 0.41 respectively, （χ² = 77.354, P <0.005） . Conclusion Among the four methods, TB-DNA has a good Sensitivity and specificity, Xpert mtbrif has a good sensitivity, TB-DNA and Xpert mtbrif ha a good authenticity, and both positive and negative predictive values are high, it has good value in the diagnosis of bone tuberculosis.
- Smear acid-fast staining,
- TB-DNA,
- Xpert/MTB/RIF,
- Culture of mycobacterium,
- Tuberculosis bone

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References(20)

References

[1]	World Health Organization. Global tuberculosis report 2016[M]. Gnenva. Switzerland: WHO Press, 2016: 1-5.
[2]	Hsiao C H,Lin Y T,Lai C C,et al. Identification of nontuberculous mycobacterial infection by IS6110 and hsp65 gene analysis on lung tissues[J]. Diagnostic Microbiology & Infectious Disease,2010,68(3):241-246.
[3]	孙宇航,王骞,施建党,等. 脊柱结核不同技术与病理标本结核分枝杆菌培养及药敏试验的比较[J]. 中国矫形外科杂志,2018,26(2):5.
[4]	董伟杰,秦世炳,兰汀隆,等. 耐多药脊柱结核的原因分析及处理对策[J]. 中华骨科杂志,2014,34(2):6.
[5]	吴弟梅. 结核病检查方法的研究进展[J]. 国际检验医学杂志,2015,36(1):106-107.
[6]	马爱静,赵雁林. 耐药结核病的流行和监测现状[J]. 中国抗生素杂志,2018,43(5):5.
[7]	李亮. 中国结核病诊疗现状及展望[C]. 第八届东北地区呼吸疾病学术会议暨吉林省医学会呼吸病学分会第十三次学术会议, 2016.
[8]	王琦璞,侍效春,刘晓清,等,. 68例骨关节结核的临床特征[J]. 中国医学科学院学报,2022,44(6):6.
[9]	Pang Y,An J,Shu W,et al. Epidemiology of extrapulmonary tuberculosis among inpatients,China,2008-2017[J]. Emerg Infect Dis,2019,25(3):457-464.
[10]	张宏其,向伟能,郭超峰,等. 噬菌体生物扩增法快速检测脊柱结核脓液中结核分枝杆菌[J]. 医学临床研究,2007,24(9):1455-1458. doi: 10.3969/j.issn.1671-7171.2007.09.006
[11]	李素华,陈莉,王欢. 荧光定量PCR检测TB-DNA在各类检样中的阳性率分析[J]. 西南国防医药,2007,17(6):752-753.
[12]	阳红梅,苏丹虹,管婧. 5种结核分枝杆菌检测方法的临床应用价值研究[J]. 国际检验医学杂志,2020,41(4):4.
[13]	沈健,郑琦,汪翼凡,等. 骨与关节结核患者病灶分枝杆菌感染分布研究[J]. 中国预防医学杂志,2019,20(11):020.
[14]	王华钧,孙小军,金法祥,等. 4种结核分枝杆菌检测方法的比较[J]. 中华医院感染学杂志,2012,22(11):3.
[15]	王震,龚玉华,钱彩娣,等. 5种结核杆菌检测方法的临床应用价值[J]. 检验医学与临床,2015,12(3):334-336. doi: 10.3969/j.issn.1672-9455.2015.03.019
[16]	韩鹏,赵丽萍,李彦芳,等. 3种方法检测痰标本结果临床应用对比分析[J]. 医学动物防制,2018,34(1):3.
[17]	邵燕琴,朱明智,戴玲珊,等. GeneXpert MTB/RIF和TB-DNA两种荧光PCR方法检测肺外结核脓液样本的效能分析[J]. 中国防痨杂志,2023,45(12):1158-1163.
[18]	Tadesse M, Abebe G, Bekele A, et al. Xpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis: a diagnostic evaluation study[J]. Clinical Microbiology and Infection, 2019, 25（8）: 1000-1005.
[19]	贾秀杰,胡小芳,程亮,等. 结核分枝杆菌/利福平耐药实时荧光定量核酸扩增检测技术对肺外结核性脓肿的诊断价值分析[J]. 现代生物医学进展,2022,22(22):4385-4389.
[20]	孙秀华,孙娇,孙炳奇. Xpert MTB/RIF检测技术和MGIT960培养在肺外结核诊断中的比较分析[J]. 当代医学,2019,25(17):45-48.

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