Identification of Atrial Fibrillation-related Inflammatory Genes and Their Association with Immune Cell Infiltration Based on Comprehensive Bioinformatic Analysis

Man YANG; Xingan ZHAO; Yunna GE; Juan QIN; Xiya WANG; Siming TAO

doi:10.12259/j.issn.2095-610X.S20240303

Volume 45 Issue 3

Mar. 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2024 > 45(3): 18-29

Man YANG, Xingan ZHAO, Yunna GE, Juan QIN, Xiya WANG, Siming TAO. Identification of Atrial Fibrillation-related Inflammatory Genes and Their Association with Immune Cell Infiltration Based on Comprehensive Bioinformatic Analysis[J]. Journal of Kunming Medical University, 2024, 45(3): 18-29. doi: 10.12259/j.issn.2095-610X.S20240303

Citation:

Man YANG, Xingan ZHAO, Yunna GE, Juan QIN, Xiya WANG, Siming TAO. Identification of Atrial Fibrillation-related Inflammatory Genes and Their Association with Immune Cell Infiltration Based on Comprehensive Bioinformatic Analysis[J]. Journal of Kunming Medical University, 2024, 45(3): 18-29. doi: 10.12259/j.issn.2095-610X.S20240303

Citation:

Man YANG, Xingan ZHAO, Yunna GE, Juan QIN, Xiya WANG, Siming TAO. Identification of Atrial Fibrillation-related Inflammatory Genes and Their Association with Immune Cell Infiltration Based on Comprehensive Bioinformatic Analysis[J]. Journal of Kunming Medical University, 2024, 45(3): 18-29. doi: 10.12259/j.issn.2095-610X.S20240303

PDF( 6526 KB)

Identification of Atrial Fibrillation-related Inflammatory Genes and Their Association with Immune Cell Infiltration Based on Comprehensive Bioinformatic Analysis

doi: 10.12259/j.issn.2095-610X.S20240303

1.
Dept. of Cardiology，Clinical Medical College，Dali University，Dali 1st People’s Hospital，Dali Yunnan 671000
2.
Dept. of Cardiology，Affiliated Hospital of Yunnan University /Yunnan 2nd People’s Hospital/ Yunnan province Eye Hospital，KunmingYunnan 650021，China

Received Date: 2023-12-12
Available Online: 2024-03-07
Publish Date: 2024-03-30

Abstract

Abstract

Objective To identify inflammation-related genes in atrial fibrillation （AF） and explore the possible role and mechanism of these genes and infiltrating immune cells in the development of AF. Methods A series of bioinformatics analysis combined with machine learning algorithms to identify biomarkers of AF, the receiver operating characteristic （ROC） curves were used to verify the prediction and diagnostic value of key genes, and Spearman correlation analysis was used to clarify the correlation between key genes and infiltrating immune cells. Results 593 differential genes （| log2 （fold change, FC） |> 1, P <0.05）, 7 immune cell subtypes （P <0.05） were selected, 190 immune-related differential genes were obtained, 3 biomarkers （IGF1, PTGS 2 and PPARG）, and the correlation analysis showed that 3 markers were significantly associated with infiltrating immune cells （P < 0.05）. Conclusion IGF1, PTGS2 and PPARG are inflammation-related genes of AF, which are speculated to be closely related to the process and pathway of immune cell infiltration.
- Atrial fibrillation,
- Bioinformatics analysis,
- Immune infiltration,
- Inflammatory response,
- Biomarkers

FullText(HTML)

References(32)

References

[1]	Zhu Y,Shi J,Zheng B,et al. Genetic findings in patients with primary fibrotic atrial cardiomyopathy[J]. European Journal of Medical Genetics,2022,65(3):104429. doi: 10.1016/j.ejmg.2022.104429
[2]	Litviňuková M,Talavera-López C,Maatz H,et al. Cells of the adult human heart[J]. Nature,2020,588(7838):466-472. doi: 10.1038/s41586-020-2797-4
[3]	Zaidi Y, Aguilar E G, Troncoso M, et al. Immune regulation of cardiac fibrosis post myocardial infarction. [Z]. 2021: 77, 109837.
[4]	Tian Y,Liu S,Zhang Y,et al. Immune infiltration and immunophenotyping in atrial fibrillation[J]. Aging (Albany NY),2023,15(1):213-229. doi: 10.18632/aging.204470
[5]	Wynn T A,Vannella K M. Macrophages in tissue repair,regeneration,and fibrosis[J]. Immunity,2016,44(3):450-462. doi: 10.1016/j.immuni.2016.02.015
[6]	Newman A M,Liu C L,Green M R,et al. Robust enumeration of cell subsets from tissue expression profiles[J]. Nat Methods,2015,12(5):453-457. doi: 10.1038/nmeth.3337
[7]	Langfelder P,Horvath S. WGCNA: An R package for weighted correlation network analysis[J]. BMC Bioinformatics,2008,9(1):559. doi: 10.1186/1471-2105-9-559
[8]	Liu Y, Shi Q, Ma Y, et al. The role of immune cells in atrial fibrillation[J]. J Mol Cell Cardiol, 2018: 123, 198-208.
[9]	Zhang Y L,Teng F,Han X,et al. Selective blocking of CXCR2 prevents and reverses atrial fibrillation in spontaneously hypertensive rats[J]. J Cell Mol Med,2020,24(19):11272-11282. doi: 10.1111/jcmm.15694
[10]	Hulsmans M,Schloss M J,Lee I H,et al. Recruited macrophages elicit atrial fibrillation[J]. Science,2023,381(6654):231-239. doi: 10.1126/science.abq3061
[11]	Grune J,Yamazoe M,Nahrendorf M. Electroimmunology and cardiac arrhythmia[J]. Nature reviews cardiology,2021,18(8):547-564. doi: 10.1038/s41569-021-00520-9
[12]	Sun Z,Zhou D,Xie X,et al. Cross-talk between macrophages and atrial myocytes in atrial fibrillation[J]. Basic Res Cardiol,2016,111(6):63. doi: 10.1007/s00395-016-0584-z
[13]	Bosco M C. Macrophage polarization: reaching across the aisle?[J]. J Allergy Clin Immunol,2019,143(4):1348-1350. doi: 10.1016/j.jaci.2018.12.995
[14]	Wen S,Yan W,Wang L. mRNA expression disturbance of complement system related genes in acute arterial thrombotic and paroxysmal atrial fibrillation patients[J]. Ann Palliat Med,2020,9(3):835-846. doi: 10.21037/apm.2020.04.18
[15]	Liu L,Zheng Q,Lee J,et al. PD-1/PD-L1 expression on CD(4+) T cells and myeloid DCs correlates with the immune pathogenesis of atrial fibrillation[J]. J Cell Mol Med,2015,19(6):1223-1233. doi: 10.1111/jcmm.12467
[16]	Cheng W L,Kao Y H,Chen Y C,et al. Macrophage migration inhibitory factor increases atrial arrhythmogenesis through CD74 signaling[J]. Transl Res,2020,216:43-56. doi: 10.1016/j.trsl.2019.10.002
[17]	Chen Y,Fu L,Pu S,et al. Systemic lupus erythematosus increases risk of incident atrial fibrillation: A systematic review and meta-analysis[J]. Int J Rheum Dis,2022,25(10):1097-1106. doi: 10.1111/1756-185X.14403
[18]	Kunamalla A,Ng J,Parini V,et al. Constitutive expression of a dominant-negative TGF-β type II receptor in the posterior left atrium leads to beneficial remodeling of atrial fibrillation substrate[J]. Circ Res,2016,119(1):69-82. doi: 10.1161/CIRCRESAHA.115.307878
[19]	Liang Y,Zhou Y,Wang J,et al. Downregulation of fibromodulin attenuates inflammatory signaling and atrial fibrosis in spontaneously hypertensive rats with atrial fibrillation via inhibiting TLR4/NLRP3 signaling pathway[J]. Immun Inflamm Dis,2023,11(10):e1003. doi: 10.1002/iid3.1003
[20]	Gao L,Kan C,Chen X,et al. Mechanism of action of Zhi Gan Cao decoction for atrial fibrillation and myocardial fibrosis in a mouse model of atrial fibrillation: A network pharmacology-based study[J]. Comput Math Methods Med,2022,2022:4525873.
[21]	Raman K,Aeschbacher S,Bossard M,et al. Whole blood gene expression differentiates between atrial fibrillation and sinus rhythm after cardioversion[J]. PLoS One,2016,11(6):e157550.
[22]	Troncoso R,Ibarra C,Vicencio J M,et al. New insights into IGF-1 signaling in the heart[J]. Trends Endocrinol Metab,2014,25(3):128-137. doi: 10.1016/j.tem.2013.12.002
[23]	Fujita M,Takada Y K,Takada Y. Insulin-like growth factor (IGF) signaling requires αvβ3-IGF1-IGF type 1 receptor (IGF1R) ternary complex formation in anchorage independence,and the complex formation does not require IGF1R and Src activation[J]. J Biol Chem,2013,288(5):3059-3069. doi: 10.1074/jbc.M112.412536
[24]	Zhao Z,Li R,Wang X,et al. Attenuation of atrial remodeling by aliskiren via affecting oxidative stress,inflammation and PI3K/Akt signaling pathway[J]. Cardiovasc Drugs Ther,2021,35(3):587-598. doi: 10.1007/s10557-020-07002-z
[25]	Cheng W,Zhu Y,Wang H. The MAPK pathway is involved in the regulation of rapid pacing-induced ionic channel remodeling in rat atrial myocytes[J]. Mol Med Rep,2016,13(3):2677-2682. doi: 10.3892/mmr.2016.4862
[26]	Dalli J,Chiang N,Serhan C N. Elucidation of novel 13-series resolvins that increase with atorvastatin and clear infections[J]. Nat Med,2015,21(9):1071-1075. doi: 10.1038/nm.3911
[27]	Kim S F,Huri D A,Snyder S H. Inducible nitric oxide synthase binds,S-nitrosylates,and activates cyclooxygenase-2[J]. Science,2005,310(5756):1966-1970. doi: 10.1126/science.1119407
[28]	Wang Z,Zeng Z,Hu Y,et al. Network pharmacology and pharmacological mechanism of CV-3 in atrial fibrillation[J]. Evid Based Complement Alternat Med,2022,2022:5496299.
[29]	Barroso I,Gurnell M,Crowley V E,et al. Dominant negative mutations in human PPARgamma associated with severe insulin resistance,diabetes mellitus and hypertension[J]. Nature,1999,402(6764):880-883. doi: 10.1038/47254
[30]	Park S H,Choi H J,Yang H,et al. Endoplasmic reticulum stress-activated C/EBP homologous protein enhances nuclear factor-kappaB signals via repression of peroxisome proliferator-activated receptor gamma[J]. J Biol Chem,2010,285(46):35330-35339. doi: 10.1074/jbc.M110.136259
[31]	Chen Y L, Chuang J H, Wang H T, et al. Altered expression of circadian clock genes in patients with atrial fibrillation is associated with atrial high-rate episodes and left atrial remodeling[J]. Diagnostics （Basel）, 2021, 11（1）: 90.
[32]	Wang N,Yang G,Jia Z,et al. Vascular PPARgamma controls circadian variation in blood pressure and heart rate through Bmal1[J]. Cell Metab,2008,8(6):482-491. doi: 10.1016/j.cmet.2008.10.009

Relative Articles(20)

Relative Articles

[1]	Wanshu LIU, Furong DENG, Ping YANG, Xin TIAN, Guangli ZHOU, Lingmin ZHAO, Xueting ZHANG, Keyi ZHANG. A Study on the Related Factors of Atrial Fibrillation in Patients with Type 2 Diabetes. Journal of Kunming Medical University, 2025, 46(5): 1-7.
[2]	Ji WANG, Songtao BIN, Li TAN. Study on the Efficacy of Acetylcysteine in Severe Pneumonia in Children under Electronic Bronchoscopy. Journal of Kunming Medical University, 2024, 45(6): 113-119. doi: 10.12259/j.issn.2095-610X.S20240615
[3]	Li LI, Ruixian ZHANG. Research Progress on The Potential Application of Circular RNA in Systemic Lupus Erythematosus. Journal of Kunming Medical University, 2024, 45(6): 161-165. doi: 10.12259/j.issn.2095-610X.S20240622
[4]	Daolin CUI, Chunli CHEN, Zhenghong ZHOU, Lei GONG. Bioinformatics-Based Analysis of the Roles of MX1，IFI44， and STAT1 in Lupus Nephritis. Journal of Kunming Medical University, 2024, 45(12): 1-10.
[5]	Ying ZHANG, Xiangru LUO, Xing WANG, Pan ZHAO, Kun LIAN, Yuanqin LI, Mingkun SHAO. Analysis on the Expression of Basement Membrane Protein in Endometrial Carcinoma and Its Relationship with Immune Invasion. Journal of Kunming Medical University, 2024, 45(12): 1-10.
[6]	Zilin MA, Wenni MA, Yi MA, Yongshi FAN, Yan JIN, Ying HUANG. Investigation and Analysis of Health Education Needs of Patients Undergoing Radiofrequency Ablation of Atrial Fibrillation Based on Kano Model. Journal of Kunming Medical University, 2024, 45(2): 170-176. doi: 10.12259/j.issn.2095-610X.S20240225
[7]	Yan ZHANG, Haojie ZHAO, Mingzhi DUAN, Jingyan DUAN, Xueyan YANG. Interventional Effect and Mechanism of Saikosaponin A on Noise-induced Tinnitus in Mice. Journal of Kunming Medical University, 2023, 44(11): 29-37. doi: 10.12259/j.issn.2095-610X.S20231105
[8]	Yuxin XIONG, Ying YANG. Research Progress of Diabetic Tubulopathy. Journal of Kunming Medical University, 2023, 44(9): 148-154. doi: 10.12259/j.issn.2095-610X.S20230920
[9]	Xiaodong ZHU, Yanlong LIU, Xu ZHOU, Hailong DAI, Xiaolong YIN. Value of cMyBP-C in Clinical Diagnosis of Acute Myocardial Infarction. Journal of Kunming Medical University, 2023, 44(3): 97-102. doi: 10.12259/j.issn.2095-610X.S20230328
[10]	Yaling ZHAO, Kun WU, Kai WANG, Rong HUANG, Genya HE, Yuhui NA, Miao HE, Zhenbo DING, Caiying ZHANG. Influence of Intrauterine Hyperglycemia Environment on Peripheral Inflammatory Response in Offspring of Gestational Diabetes Mellitus. Journal of Kunming Medical University, 2023, 44(11): 70-75. doi: 10.12259/j.issn.2095-610X.S20231110
[11]	Zhongshan ZHU, Zhou YANG, Chengchuan JIANG, Xiaobing LI, Dou REN, Cheng HUANG, Weiwei ZHANG, Xiangjun LI, Shunli ZHAO. An Analysis of PLA2G1B Expression and Prognosis in Patients with Lung Adenocarcinoma. Journal of Kunming Medical University, 2022, 43(9): 70-76. doi: 10.12259/j.issn.2095-610X.S20220912
[12]	Li-yuan AN, Bing LI, Gang SU. Effects of Different Doses of Ulinastatinon Postoperative Cognitive Function and HMGB1 in Elderly Patients. Journal of Kunming Medical University, 2021, 42(7): 121-125. doi: 10.12259/j.issn.2095-610X.S20210720
[13]	Jun-jie LI, Hai-yan JIANG, Wen-ya BAI, Si-ying HUO, Zhi-sheng SUN, Jian-lin SHAO. Effects of Silencing RND3 Expression on the Inflammatory Response and Apoptosis of Hippocampal Neurons Injured by Oxygen Glucose Deprivation/Reoxygenation. Journal of Kunming Medical University, 2021, 42(10): 14-21. doi: 10.12259/j.issn.2095-610X.S20211012
[14]	Hao LI, Jing WANG, Ping YANG. Research Progress of Atrial Fibrillation and Atrial Metabolic Remodeling. Journal of Kunming Medical University, 2021, 42(3): 149-154. doi: 10.12259/j.issn.2095-610X.S20210335
[15]	Wang Guang , Yang Tong Xin , Jiang Yong Ming , Fang Ke Wei , Liu Jian He , Li Jiong Ming . . Journal of Kunming Medical University, 2019, 40(06): 23-27.
[16]	Wei Wei , Yang Bei , Han Ming Hua , Tao Si Ming , Yang Zhi Gang . . Journal of Kunming Medical University, 2019, 40(10): 121-124.
[17]	Liu Ya Ming , Xu Mian , Yan Yue Xin , Zhou Feng Gao , Xu Cheng , Zhao Kun , Jiang Guo Yun , Wu Yu, Liu Rong . . Journal of Kunming Medical University, 2019, 40(06): 16-22.
[18]	Li Fei . Effect of Celebrex on the Hemorheology and Levels of Inflammatory Factors in Patients with Delayed Fracture Healing. Journal of Kunming Medical University,
[19]	. Clinical Research of Catheter Ablation for 60 Patients with Paroxysmal Atrial Fibrillation. Journal of Kunming Medical University,
[20]	Hao Ying Lu . . Journal of Kunming Medical University,