Propofol Regulates MPP+-induced Mitochondrial Oxidative Stress and Apoptosis in SH-SY5Y Cells

Ying TAN; Haiyan QIN; Xiang SUN; Yanyi SU; Yingbao WANG

doi:10.12259/j.issn.2095-610X.S20240305

Volume 45 Issue 3

Mar. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(3): 35-41

Ying TAN, Haiyan QIN, Xiang SUN, Yanyi SU, Yingbao WANG. Propofol Regulates MPP+-induced Mitochondrial Oxidative Stress and Apoptosis in SH-SY5Y Cells[J]. Journal of Kunming Medical University, 2024, 45(3): 35-41. doi: 10.12259/j.issn.2095-610X.S20240305

Citation:

Ying TAN, Haiyan QIN, Xiang SUN, Yanyi SU, Yingbao WANG. Propofol Regulates MPP+-induced Mitochondrial Oxidative Stress and Apoptosis in SH-SY5Y Cells[J]. Journal of Kunming Medical University, 2024, 45(3): 35-41. doi: 10.12259/j.issn.2095-610X.S20240305

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Propofol Regulates MPP+-induced Mitochondrial Oxidative Stress and Apoptosis in SH-SY5Y Cells

doi: 10.12259/j.issn.2095-610X.S20240305

1.
Dept. of Anesthesiology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
Dept. of Urology，Yan’an Hospital of Kunming City，Kunming Yunnan 650051，China

Received Date: 2023-12-25
Available Online: 2024-03-11
Publish Date: 2024-03-30

Abstract

Abstract

Objective To investigate the effects of different concentrations of PPF on oxidative stress and apoptosis of PD model cells induced by MPP+. Methods The human neuroblastoma cell SH-SY5Y was induced by 1 mM MPP+ to establish PD cell model. In PPF treatment group, SH-SY5Y cells were stimulated with 10, 20, 40 and 80 μM PPF for 4 h before MPP+ induction. Cell counting kit-8（CCK-8） was performed to evaluate cell proliferation activity. H2DCF-DA fluorescent probe was used to detect ROS in cells. The levels of MDA and NADPH oxidase were analyzed by the kit. Western blot examined the protein expression of cytochrome c in mitochondria and cytoplasm, as well as the relative expression of Bcl-2, Bax and cleaved caspase-3 in SH-SY5Y cells. Apoptosis rate was analyzed by flow cytometry. Results MPP+ significantly inhibited the proliferation of SH-SY5Y cells（P < 0.001）, promoted the level of ROS（P < 0.001）, MDA（P < 0.001）, NADPH oxidase（P < 0.01）, cytochrome c in cytoplasm（P < 0.01） and induced apoptosis（P < 0.001） and the relative expression of pro-apoptosis protein Bax and cleaved caspase-3（P < 0.01）, reduced cytochrome c protein in mitochondria（P < 0.01） and the relative expression of anti-apoptosis protein（P < 0.01）. PPF pretreatment alleviated the proliferation inhibition, oxidative stress and apoptosis promotion of SH-SY5Y cells induced by MPP+（P < 0.001）, and the effects of 40 μM and 80 μM on cells were more significant. Conclusion PPF pretreatment can alleviate the oxidative stress of SH-SY5Y cells induced by MMP+ and reduce apoptosis rate.
- Parkinson's disease,
- Oxidative stress,
- MPP+,
- Propofol

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References(20)

References

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