Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway

Yongjun DENG; Qian CHEN; Jianbin ZOU; Zheng GONG; Huanpeng LIU

doi:10.12259/j.issn.2095-610X.S20240405

Volume 45 Issue 4

Apr. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(4): 35-40

Yongjun DENG, Qian CHEN, Jianbin ZOU, Zheng GONG, Huanpeng LIU. Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway[J]. Journal of Kunming Medical University, 2024, 45(4): 35-40. doi: 10.12259/j.issn.2095-610X.S20240405

Citation:

Yongjun DENG, Qian CHEN, Jianbin ZOU, Zheng GONG, Huanpeng LIU. Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway[J]. Journal of Kunming Medical University, 2024, 45(4): 35-40. doi: 10.12259/j.issn.2095-610X.S20240405

Citation:

Yongjun DENG, Qian CHEN, Jianbin ZOU, Zheng GONG, Huanpeng LIU. Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway[J]. Journal of Kunming Medical University, 2024, 45(4): 35-40. doi: 10.12259/j.issn.2095-610X.S20240405

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Overexpression of ZIC1 Gene Inhibits Proliferation of Pleural Mesothelioma Cells by Activating P53 Signaling Pathway

doi: 10.12259/j.issn.2095-610X.S20240405

1.
Dept. of Thoracic Surgery，Affiliated Hospital of Yunnan University ，Kunming Yunnan 650021
2.
School of Public Health，Dali University，Dali Yunnan 671000，China

Received Date: 2023-12-21
Available Online: 2024-04-07
Publish Date: 2024-04-29

Abstract

Abstract

Objective To investigate the effect of cerebellar zinc finger structure 1 （ZIC1） gene overexpression on the proliferation and apoptosis of pleural mesothelioma cells （SMC-1 cells） and the corresponding molecular mechanism. Methods SMC-1 cells were transfected with lentivirus particles carrying ZIC1 gene as the experimental group, infected with empty lentivirus particles as the control group, and conventional cultured untransfected SMC-1 cells as the blank control group. Western blot was used to detect the expression of ZIC1 protein in the three groups. CCK-8 cell proliferation assay was used to detect the proliferation ability of each group, and Hoechst-PI double staining was used to detect the apoptosis of each group. The protein expression levels of P53, P21, MDM2 and P53 phosphorylation site Ser392 in P53-mediated apoptosis signaling pathway were detected by Western blot. Results Compared with the empty vector group and the control group, the expression of ZIC1 protein in the ZIC1 overexpression group was significantly increased, with a statistically significant difference （F = 4.665, P = 0.036）. Compared with the control group, the proliferation ability of SMC-1 cells in the ZIC1 overexpression group was significantly reduced, while the apoptosis of tumor cells was significantly increased, with a statistically significant difference （P < 0.05）. The main genes P53, P21, MDM2, and P53 Ser392 protein expression in the P53 signaling pathway in the ZIC1 gene overexpression group were significantly increased. Conclusion Overexpression of ZIC1 gene can inhibit the proliferation of pleural mesothelioma SMC-1 cells and promote their apoptosis. The possible mechanism is to activate the P53 gene mediated apoptosis signaling pathway by upregulating the expression of P53 gene phosphorylation sites.
- ZIC1,
- Pleural mesothelioma cells,
- P53 signal pathway,
- Proliferation,
- Apoptosis

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References(19)

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