Correlation between Gut Microbiota，Serum ET，PCT Levels with the Severity and Prognosis of Sepsis

Junjie NIU; Wenjuan JI; Zhuaizhuai YU

doi:10.12259/j.issn.2095-610X.S20240420

Volume 45 Issue 4

Apr. 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2024 > 45(4): 140-145

Junjie NIU, Wenjuan JI, Zhuaizhuai YU. Correlation between Gut Microbiota，Serum ET，PCT Levels with the Severity and Prognosis of Sepsis[J]. Journal of Kunming Medical University, 2024, 45(4): 140-145. doi: 10.12259/j.issn.2095-610X.S20240420

Citation:

Junjie NIU, Wenjuan JI, Zhuaizhuai YU. Correlation between Gut Microbiota，Serum ET，PCT Levels with the Severity and Prognosis of Sepsis[J]. Journal of Kunming Medical University, 2024, 45(4): 140-145. doi: 10.12259/j.issn.2095-610X.S20240420

Citation:

PDF( 712 KB)

Correlation between Gut Microbiota，Serum ET，PCT Levels with the Severity and Prognosis of Sepsis

doi: 10.12259/j.issn.2095-610X.S20240420

Dept. of Clinical Laboratory，Jincheng General Hospital，Jincheng Shanxi 048006，China

Received Date: 2023-09-25
Available Online: 2024-04-03
Publish Date: 2024-04-29

Abstract

Abstract

Objective To investigate the correlation between the levels of intestinal flora, serum endotoxin （ET）, and procalcitonin （PCT） in patients with sepsis and their disease severity and prognosis. Methods A total of 373 patients with sepsis in Jincheng University Hospital from January 2016 to December 2022 were selected and divided into sepsis group （n=261） and sepsis shock group （n=112） according to the severity of sepsis. The general data, intestinal flora, serum ET and PCT levels were compared between the two groups, and the serum ET and PCT levels were compared among patients with different degrees of intestinal flora disturbance. The relationship between serum PCT and ET levels and the degree of intestinal flora disturbance in sepsis was analyzed.The prognosis of patients for 28 days was statistically analyzed, and the intestinal flora, serum ET, and PCT levels at the time of admission were compared between dead and alive patients to analyze the value of intestinal flora, serum ET, and PCT levels in predicting prognosis. Results Patients in the septic shock group had lower counts of bifidobacteria and lactobacilli compared to the sepsis group, while counts of Escherichia coli, enterococci, as well as serum levels of ET and PCT were higher in the septic shock group. The severity of disruption in gut microbiota was associated with higher serum levels of ET and PCT, with level III patients having higher levels than level II and level I patients. The levels of ET and PCT in septic patients were positively correlated with the severity of disruption in gut microbiota. Upon admission, deceased patients had lower counts of bifidobacteria and lactobacilli compared to surviving patients, while counts of enterococci, Escherichia coli, as well as serum levels of ET and PCT were higher in deceased patients. The predictive value for prognosis of death for bifidobacteria, enterococci, Escherichia coli, lactobacilli, ET, and PCT were 0.788, 0.782, 0.787, 0.768, 0.791, and 0.776 respectively. The combined predictive value had the highest AUC of 0.928. Conclusion The intestinal flora, serum ET, and PCT levels in patients with sepsis are closely related to the severity of the disease and have good application value in predicting prognosis.
- Sepsis,
- Intestinal microbiota,
- Endotoxins,
- Procalcitonin,
- Prognosis

FullText(HTML)

References(21)

References

[1]	Liu D,Huang S Y,Sun J H,et al. Sepsis-induced immunosuppression: Mechanisms,diagnosis and current treatment options[J]. Mil Med Res,2022,9(1):56.
[2]	Stephen A H,Montoya R L,Aluisio A R. Sepsis and septic shock in low- and middle-income countries[J]. Surg Infect (Larchmt),2020,21(7):571-578. doi: 10.1089/sur.2020.047
[3]	Ackerman M H,Ahrens T,Kelly J,et al. Sepsis[J]. Crit Care Nurs Clin North Am,2021,33(4):407-418. doi: 10.1016/j.cnc.2021.08.003
[4]	杨小娟,杨晓军,刘丹,等. 脓毒症患者肠道菌群与肠屏障功能紊乱的相关性研究[J]. 中华急诊医学杂志,2022,31(2):210-216. doi: 10.3760/cma.j.issn.1671-0282.2022.02.013
[5]	Yang X J,Liu D,Ren H Y,et al. Effects of sepsis and its treatment measures on intestinal flora structure in critical care patients[J]. World J Gastroenterol,2021,27(19):2376-2393. doi: 10.3748/wjg.v27.i19.2376
[6]	Lee M J,Bae J,Lee J H,et al. Serial change of endotoxin tolerance in a polymicrobial sepsis model[J]. Int J Mol Sci,2022,23(12):6581. doi: 10.3390/ijms23126581
[7]	王超,张荣丽. ICU脓毒血症患者细菌内毒素水平与多种炎症因子的比较[J]. 热带医学杂志,2020,20(8):1070-1073,封3. doi: 10.3969/j.issn.1672-3619.2020.08.020
[8]	中国医师协会急诊医师分会,中国研究型医院学会休克与脓毒症专业委员会. 中国脓毒症/脓毒性休克急诊治疗指南(2018)[J]. 中国急救医学,2018,38(9):741-756. doi: 10.3969/j.issn.1002-1949.2018.09.001
[9]	顾晓霞,高振军. 溃疡性结肠炎患者肠道菌群失调与H. pylori感染和DAI评分及免疫球蛋白水平的相关性[J]. 中国微生态学杂志,2023,35(3):348-352.
[10]	杨银娟,尚亚楠,尚向涛. 脓毒症患者肠道细菌紊乱与炎性细胞因子及T淋巴细胞亚群的相关性[J]. 中国急救复苏与灾害医学杂志,2020,15(9):1092-1095. doi: 10.3969/j.issn.1673-6966.2020.09.023
[11]	Li H,Xie J Y,Guo X L,et al. Bifidobacterium spp. and their metabolite lactate protect against acute pancreatitis via inhibition of pancreatic and systemic inflammatory responses[J]. Gut Microbes,2022,14(1):2127456. doi: 10.1080/19490976.2022.2127456
[12]	Han S C,Zheng H T,Han F,et al. Lactobacillus johnsonii 6084 alleviated sepsis-induced organ injury by modulating gut microbiota[J]. Food Sci Nutr,2022,10(11):3931-3941. doi: 10.1002/fsn3.2989
[13]	Stoll B J,Puopolo K M,Hansen N I,et al. Early-onset neonatal sepsis 2015 to 2017,the rise of Escherichia coli,and the need for novel prevention strategies[J]. JAMA Pediatr,2020,174(7):e200593. doi: 10.1001/jamapediatrics.2020.0593
[14]	Huang X Q,Qiu J K,Wang C H,et al. Sepsis secondary to multifocal Enterococcus faecium infection: A case report[J]. Medicine (Baltimore),2020,99(27):e19811.
[15]	关岚,周衡,王聪,等. 脓毒症患者肠道菌群多样性和结构变化分析[J]. 中华急诊医学杂志,2022,31(4):514-519. doi: 10.3760/cma.j.issn.1671-0282.2022.04.015
[16]	邵瑞飞,杨艳,郑志榕,等. 肠道菌群和“肠-肺”轴在脓毒症中的作用[J]. 基础医学与临床,2020,40(8):1109-1112.
[17]	陈君,王妮,陈栩栩,等. ICU脓毒症患者血清Presepsin、内毒素、IL-6、PCT水平与预后的相关性[J]. 中华医院感染学杂志,2022,32(3):356-359.
[18]	张赟,陈娟,黄尧米. 血浆Th1/Th2细胞、ET、PTA、PCT与脓毒症患儿病情严重程度及转归的关系分析[J]. 河北医科大学学报,2022,43(12):1449-1453. doi: 10.3969/j.issn.1007-3205.2022.12.016
[19]	刘莉,吴晓颖,李兴泉. 脓毒症患者中可溶性髓样细胞触发受体1、降钙素原、核因子κB水平与肠道菌群失调的相关性[J]. 微生物与感染,2021,16(4):256-260. doi: 10.3969/j.issn.1673-6184.2021.04.005
[20]	Bauer M,Gerlach H,Vogelmann T,et al. Mortality in sepsis and septic shock in Europe,North America and Australia between 2009 and 2019- results from a systematic review and meta-analysis[J]. Crit Care,2020,24(1):239. doi: 10.1186/s13054-020-02950-2
[21]	Font M D,Thyagarajan B,Khanna A K. Sepsis and septic shock - basics of diagnosis,pathophysiology and clinical decision making[J]. Med Clin North Am,2020,104(4):573-585. doi: 10.1016/j.mcna.2020.02.011

Relative Articles(20)

Relative Articles

[1]	Yuanyuan LI, Yaxian SONG, Yushan XU, Xiaofu ZENG, Hui YUAN, Zhao XU, Yan JIANG. The Association of Intestinal Flora Metabolite TMAO with Non-alcoholic Fatty Liver Disease. Journal of Kunming Medical University, 2024, 45(2): 77-84. doi: 10.12259/j.issn.2095-610X.S20240210
[2]	Lin XU, Kaicheng GAO, Jie JIA, Yuyang LI, Huawei WANG, Yiqun KUANG, Yu ZHAO. Effect of Shen-ling-bai-zhu-san on Methamphetamine-Induced Gut Microbiota Change in Mice. Journal of Kunming Medical University, 2023, 44(8): 37-43. doi: 10.12259/j.issn.2095-610X.S20230829
[3]	Caihong LIANG, Mingyao MENG, Xinxin LI, Jingjing XIONG, Meng LI, Mei LIU, Zongliu HOU, Yongkun HUANG. Effect of Intestinal Flora Metabolites Deoxycholic Acid on the Proliferation and Cell Cycle of Human Umbilical Cord Mesenchymal Stem Cells. Journal of Kunming Medical University, 2023, 44(4): 23-30. doi: 10.12259/j.issn.2095-610X.S20230402
[4]	Shaoyou DENG, Yulan ZHAO, Peijin WANG, Rong LI, Jintao LI, Hong ZHENG. Effects of Osteoking Combined with Antibiotic Cocktail on Insulin Resistance and Gut Flora in db/db Mice. Journal of Kunming Medical University, 2023, 44(5): 12-18. doi: 10.12259/j.issn.2095-610X.S20230530
[5]	Saiqiong ZHANG, Wuquan LI, Qingjiang CHEN. Clinical Efficacy of Continuous Renal Replacement Therapy in Severe Burn Sepsis. Journal of Kunming Medical University, 2023, 44(11): 94-99. doi: 10.12259/j.issn.2095-610X.S20231114
[6]	Lu LI, Yunfen TIAN. Research Progress on Intestinal Microflora and Non-alcoholic Fatty Liver Disease in Children. Journal of Kunming Medical University, 2023, 44(7): 148-155. doi: 10.12259/j.issn.2095-610X.S20230708
[7]	Tuan LI, Yu’e ZHOU, Li MA, Gui LIAO. Clinical Significance of C Reactive Protein and Procalcitonin Detection in Neonatal ABO Hemolytic Jaundice. Journal of Kunming Medical University, 2023, 44(1): 80-84. doi: 10.12259/j.issn.2095-610X.S20230122
[8]	Yaping JIANG, Hongying YANG, Haohan WANG, Xianling Ning, Xielan YANG. Effect of Different Intestinal Surgical Methods on Prognosis of Patients with Intestinal Invasive Epithelial Ovarian Cancer. Journal of Kunming Medical University, 2023, 44(1): 85-90. doi: 10.12259/j.issn.2095-610X.S20230109
[9]	Dan LI, Xulian WAN, Lvyu LI, Yu YUN, Guangyun LUO, Weibing LIU, Gongfu LIN, Ning LI, Yongkun LI, Weigang DUAN. Intestinal Flora in Uricase-Deficient Rats. Journal of Kunming Medical University, 2023, 44(2): 27-32. doi: 10.12259/j.issn.2095-610X.S20230205
[10]	Hong WANG, Dexing YANG, Qiang WANG, Weiyu ZHOU, Jiefu TANG, Zhenfang WANG, Kai FU, Shengzhe LIU, Rong LIU. Risk Factors Analysis and Prediction Model Establishment of Refeeding Syndrome in ICU Patients with Sepsis. Journal of Kunming Medical University, 2022, 43(11): 44-51. doi: 10.12259/j.issn.2095-610X.S20221102
[11]	Sixiang LIU, Yongkun HUANG, Mingying WANG, Hongwei HU, Min MA, Yu LING. Analysis of Intestinal Flora and Therapeutic Intervention in Children with Functional Constipation. Journal of Kunming Medical University, 2022, 43(3): 123-127. doi: 10.12259/j.issn.2095-610X.S20220309
[12]	Wei YUAN, Mingyao MENG, Xinxin LI, Jingjing XIONG, Meng LI, Jia CAO, Mei LIU, Zongliu HOU, Yongkun HUANG. Effects of Dose-response and Time-dependent of Endotoxin on Proliferation of Human Umbilical Cord Mesenchymal Stem Cells. Journal of Kunming Medical University, 2022, 43(9): 7-12. doi: 10.12259/j.issn.2095-610X.S20220928
[13]	Liang Rui , Chun Yu Wei Xun , Shen Dao , Sun Le , Li Yun Feng . . Journal of Kunming Medical University, 2020, 41(06): 156-161.
[14]	Ping-yan XUE, Yan JIANG, Yu-shan XU, Hui YUAN, Xuan LI, Ya-xian SONG, Hua LIU. Changes of Intestinal Flora in Non-alcoholic Fatty Liver Disease. Journal of Kunming Medical University, 2020, 41(11): 62-67. doi: 10.12259/j.issn.2095-610X.S20201120
[15]	Liu Ya Ming , Xu Mian , Yan Yue Xin , Zhou Feng Gao , Xu Cheng , Zhao Kun , Jiang Guo Yun , Wu Yu, Liu Rong . . Journal of Kunming Medical University, 2019, 40(06): 16-22.
[16]	Jie Chun Lin , Huang Tao , Teng Zhao Wei , Bu Jun Hui , Li Meng Zhou , Zhang Jing. . Journal of Kunming Medical University, 2019, 40(04): 91-96.
[17]	Xu Jian , Wang Zi Yu , Chen Yao Xiang , Mou Bo , Jin Yan , Ma Lei . . Journal of Kunming Medical University, 2019, 40(04): 97-100.
[18]	Teng Yan . Effect of Hemoperfusion on Inflammation Factors in Patients with Sepsis. Journal of Kunming Medical University,
[19]	Miao Yu Lan . . Journal of Kunming Medical University,
[20]	Miao Yu Lan . . Journal of Kunming Medical University,