Researches on the Mechanism of Overexpression of Tripartite Motif Protein 48 Regulating p-ERK1/2 to Inhibit Glioma Growth

Youchuan JIANG; Yan YU; Guo ZHAO; Shicun LI; Peng DING

doi:10.12259/j.issn.2095-610X.S20240505

Volume 45 Issue 5

May 2024

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Youchuan JIANG, Yan YU, Guo ZHAO, Shicun LI, Peng DING. Researches on the Mechanism of Overexpression of Tripartite Motif Protein 48 Regulating p-ERK1/2 to Inhibit Glioma Growth[J]. Journal of Kunming Medical University, 2024, 45(5): 29-36. doi: 10.12259/j.issn.2095-610X.S20240505

Citation:

Youchuan JIANG, Yan YU, Guo ZHAO, Shicun LI, Peng DING. Researches on the Mechanism of Overexpression of Tripartite Motif Protein 48 Regulating p-ERK1/2 to Inhibit Glioma Growth[J]. Journal of Kunming Medical University, 2024, 45(5): 29-36. doi: 10.12259/j.issn.2095-610X.S20240505

Citation:

Youchuan JIANG, Yan YU, Guo ZHAO, Shicun LI, Peng DING. Researches on the Mechanism of Overexpression of Tripartite Motif Protein 48 Regulating p-ERK1/2 to Inhibit Glioma Growth[J]. Journal of Kunming Medical University, 2024, 45(5): 29-36. doi: 10.12259/j.issn.2095-610X.S20240505

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Researches on the Mechanism of Overexpression of Tripartite Motif Protein 48 Regulating p-ERK1/2 to Inhibit Glioma Growth

doi: 10.12259/j.issn.2095-610X.S20240505

Dept. of Neurosurgery，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2024-01-15
Available Online: 2024-04-30
Publish Date: 2024-05-31

Abstract

Abstract

Objective To investigate the impact of TRIM48 overexpression on glioma and explore the underlying mechanism. Methods Twelve nude mice were randomly assigned into two groups: one inoculated with U87 glioma cells stably overexpressing TRIM48 （oeTRIM48 group） and the other with the control cell line （Vector group）. Tumor volume was measured every 3 days post-inoculation, and after 4 weeks, the tumors were excised and weighed. Tumor tissues underwent hematoxylin and eosin （H&E） staining, and Ki-67 expression was assessed using immunofluorescence. Additionally, the expressions of TRIM48, ERK1/2, and phosphorylated ERK1/2 （p-ERK1/2） in the tumors of nude mice and human glioma tissue arrays were analyzed through Western blot and immunohistochemistry respectively. Results Compared to the Vector group, the oeTRIM48 group exhibited significantly reduced tumor volume and weight （P < 0.0001）. H&E staining indicated a decrease in nuclei and mitotic count in the oeTRIM48 group. Furthermore, Ki-67 expression was significantly lower in the oeTRIM48 group than that in the Vector group （P < 0.0001）. The oeTRIM48 group also showed a significantly lower expression of p-ERK1/2 protein compared to the Vector group （P < 0.01）. Immunohistochemistry on tissue microarrays revealed the high expression of TRIM48 and low expression of p-ERK1/2 in adjacent non-tumoral tissues, with the opposite pattern observed in tumor tissues. Conclusion Overexpression of TRIM48 inhibits the growth and proliferation of glioma, potentially through modulation of the ERK1/2 signaling pathway.
- TRIM48,
- Glioma,
- ERK1/2

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References(29)

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