The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury

Guojing LIANG; Kun JI; Kaichun ZHANG; Yufang ZHANG; Jing AN; Yuge ZHANG; Juan WEN; Haiyan REN

doi:10.12259/j.issn.2095-610X.S20240506

Volume 45 Issue 5

May 2024

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Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506

Citation:

Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506

Citation:

Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury[J]. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506

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The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury

doi: 10.12259/j.issn.2095-610X.S20240506

1.
The Basic Medical Department of Xinjiang Medical University，Urumqi Xinjiang 830011
2.
Dept. of Neurosurgery，The 6th Affiliated Hospital of Xinjiang Medical University，Urumqi Xinjiang 830011
3.
Dept. of Cardiovascular Medicine，Urumqi Friendship Hospital，Urumqi Xinjiang 830011
4.
PCR Biology Laboratory of Xinjiang Medical University，Nucleic Acid Detection Center of Xinjiang Uygur Autonomous Region; Urumqi Xinjiang 830011
5.
Dept. of Pathology ， Changge Municipal People’s Hospital，Changge Henan 461500
6.
Central Laboratory of laboratory and Equipment Management Division of Xinjiang Medical University，Urumqi Xinjiang 830011，China

Received Date: 2024-01-05
Available Online: 2024-04-30
Publish Date: 2024-05-31

Abstract

Abstract

Objective To explore the effect of probiotics interventionon of the expression of Aβ in rats with cerebral ischemia-reperfusion and the protective effect of neurons. Methods Male SD rats were selected as the experimental animals and randomly divided into Sham Group, Model Group, Probiotics Group and Edaravone Group. The common carotid artery was separated only to the Sham Group While a cerebral ischemia-reperfusion model was eatablished to the other groups. The degree of brain injury was evaluated using neurobehavioral scoring; The infarct area of rat brain tissue was observed using TTC staining; HE staining was used to observe the pathological and morphological changes of neurons in the CA1 and cortical regions of the hippocampus in rats; The expression of Aβ proteins was detected using immunohistochemistry experiments. Results Compared with the Sham Group, the Model Group showed an increase in neurobehavioral scores （P < 0.05）, an increase in cerebral infarction focus （P < 0.05）, neuronal damage in the hippocampal CA1 and cortical regions, and a significant upregulation of Aβ protein expression （P < 0.001, P < 0.05）; Compared with the Model Group, the Probiotics Group and Edaravone Group showed a decrease in neurobehavioral scores （P < 0.05） and a reduction in cerebral infarction focus （P < 0.05）. The Probiotic Group and the Edaravone Group showed the less neuronal damage in the hippocampal CA1 and cortical regions , and the Aβ protein expression levels were significantly down regulated in both groups （P < 0.001, P < 0.05）. The improvement was more significant in Edaravone Group. Conclusion Probiotics intervention can downregulate the expression of Aβ protein, alleviate the brain tissue damage and exert neuroprotective effects in rats with cerebral ischemia-reperfusion.
- Probiotics,
- Cerebral ischemia-reperfusion injury,
- β amyloid,
- Edaravone

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References(30)

References

[1]	Savitz S I,Baron J C,Yenari M A,et al. Reconsidering neuroprotection in the reperfusion era[J]. Stroke,2017,48(12):3413-3419. doi: 10.1161/STROKEAHA.117.017283
[2]	Pawar A,Pardasani K R. Mechanistic insights of neuronal calcium and ip3 signaling system regulating atp release during ischemia in progression of Alzheimer's disease[J]. Eur Biophys J,2023,52(3):153-173. doi: 10.1007/s00249-023-01660-1
[3]	Ren H,Ma L,Gong X,et al. Edaravone exerts brain protective function by reducing the expression of aqp4,app and aβ proteins[J]. Open Life Sci,2019,14(1):651-658. doi: 10.1515/biol-2019-0074
[4]	Pluta R,Ułamek-Kozioł M,Januszewski S,et al. Participation of amyloid and tau protein in neuronal death and neurodegeneration after brain ischemia[J]. Int J Mol Sci,2020,21(13):4599. doi: 10.3390/ijms21134599
[5]	Ułamek-Kozioł M,Czuczwar S J,Januszewski S,et al. Proteomic and genomic changes in tau protein,which are associated with Alzheimer's disease after ischemia-reperfusion brain injury[J]. Int J Mol Sci,2020,21(3):892. doi: 10.3390/ijms21030892
[6]	Nguyen T-V V,Hayes M,Zbesko J C,et al. Alzheimer's associated amyloid and tau deposition co-localizes with a homeostatic myelin repair pathway in two mouse models of post-stroke mixed dementia[J]. Acta Neuropathol Commun,2018,6(1):100. doi: 10.1186/s40478-018-0603-4
[7]	Wang J,Zhang H,He J,et al. The role of the gut microbiota in the development of ischemic stroke[J]. Front Immunol,2022,28(13):845243.
[8]	Benakis C,Poon C,Lane D,et al. Distinct commensal bacterial signature in the gut is associated with acute and long-term protection from ischemic stroke[J]. Stroke,2020,51(6):1844-1854. doi: 10.1161/STROKEAHA.120.029262
[9]	Li H,Sun J,Du J,et al. Clostridium butyricum exerts a neuroprotective effect in a mouse model of traumatic brain injury via the gut-brain axis[J]. Neurogastroenterol Motil,2018,30(5):e13260. doi: 10.1111/nmo.13260
[10]	冯云,亢君君,方宗平,等. 肠道菌群移植通过降低il-17水平减轻老年小鼠脑缺血再灌注损伤[J]. 细胞与分子免疫学杂志,2019,35(1):52-57.
[11]	Wang H,Ren S,Lv H,et al. Gut microbiota from mice with cerebral ischemia-reperfusion injury affects the brain in healthy mice[J]. Aging (Albany NY),2021,13(7):10058-10074. doi: 10.18632/aging.202763
[12]	Wu H,Chiou J. Potential benefits of probiotics and prebiotics for coronary heart disease and stroke[J]. Nutrients,2021,13(8):2878. doi: 10.3390/nu13082878
[13]	Pan F,Zhang L,Li M,et al. Predominant gut lactobacillus murinus strain mediates anti-inflammaging effects in calorie-restricted mice[J]. Microbiome,2018,6(1):54. doi: 10.1186/s40168-018-0440-5
[14]	Akhoundzadeh K,Vakili A,Shadnoush M,et al. Effects of the oral ingestion of probiotics on brain damage in a transient model of focal cerebral ischemia in mice[J]. Iran J Med Sci,2018,43(1):32-40.
[15]	刘兆兰,刘宝全,李兰花,等. 动态随机分组方法介绍及应用[J]. 中西医结合学报,2011,9(3):246-251.
[16]	梁国晶,任海燕,张钰鸽,等. 红茶菌在脑缺血再灌注损伤大鼠模型中的作用研究[J]. 现代生物医学进展,2023,23(3):428-432.
[17]	卢小叶,吕倩忆,李棋龙,等. Zea-longa评分与改良Garcia评分应用于针刺治疗CIRI大鼠神经功能缺损评估的研究[J]. 湖南中医药大学学报,2021,41(9):1356-1360.
[18]	Li Y,Zhang J. Animal models of stroke[J]. Animal Model Exp Med,2021,4(3):204-219. doi: 10.1002/ame2.12179
[19]	Maida C D,Norrito R L,Daidone M,et al. Neuroinflammatory mechanisms in ischemic stroke: Focus on cardioembolic stroke,background,and therapeutic approaches[J]. Int J Mol Sci,2020,21(18):6454. doi: 10.3390/ijms21186454
[20]	Dai S J,Zhang J Y,Bao Y T,et al. Intracerebroventricular injection of aβ1-42 combined with two-vessel occlusion accelerate Alzheimer's disease development in rats[J]. Pathol Res Pract,2018,214(10):1583-1595. doi: 10.1016/j.prp.2018.07.020
[21]	Pluta R,Miziak B,Czuczwar S J. Post-ischemic permeability of the blood-brain barrier to amyloid and platelets as a factor in the maturation of Alzheimer's disease-type brain neurodegeneration[J]. Int J Mol Sci,2023,24(13):10739. doi: 10.3390/ijms241310739
[22]	Loh J S, Mak W Q, Tan L K S, et al. Microbiota-gut-brain axis and its therapeutic applications in neurodegenerative diseases[J]. Signal Transduct Target Ther,2024,9(1):37. Published 2024 Feb 16.
[23]	Abdelhamid M,Zhou C,Ohno K,et al. Probiotic bifidobacterium breve prevents memory impairment through the reduction of both amyloid-β production and microglia activation in app knock-in mouse[J]. J Alzheimers Dis,2022,85(4):1555-1571. doi: 10.3233/JAD-215025
[24]	王亚南, 陈真珍, 王凯华, 等. 补阳壮通饮对脑缺血再灌注损伤大鼠的神经保护作用及其可能机制研究[J]. 医学研究杂志,2024,53(4):40-45.
[25]	杨梅芳,程萍,陈治任,等. 肠道菌群代谢产物TMAO激活HMGB1/NLRP3炎症通路促进小鼠脑缺血半暗带损伤的机制研究[J]. 中风与神经疾病杂志,2023,40(12):1096-1100.
[26]	Gilbert K,Arseneault-Br é ard J,Flores Monaco F,et al. Attenuation of post-myocardial infarction depression in rats by n-3 fatty acids or probiotics starting after the onset of reperfusion[J]. Br J Nutr,2013,109(1):50-56. doi: 10.1017/S0007114512003807
[27]	Rezaeiasl Z,Salami M,Sepehri G. The effects of probiotic lactobacillus and bifidobacterium strains on memory and learning behavior,long-term potentiation (ltp),and some biochemical parameters in β-amyloid-induced rat's model of alzheimer's disease[J]. Prev Nutr Food Sci,2019,24(3):265-273. doi: 10.3746/pnf.2019.24.3.265
[28]	Kim M S,Kim Y,Choi H,et al. Transfer of a healthy microbiota reduces amyloid and tau pathology in an Alzheimer's disease animal model[J]. Gut,2020,69(2):283-294. doi: 10.1136/gutjnl-2018-317431
[29]	任海燕,赵晖,王蕾,等. 依达拉奉对脑缺血再灌注后aβ及其前体表达干预[J]. 科技导报,2015,33(12):77-82.
[30]	Ye T,Yuan S,Kong Y,et al. Effect of probiotic fungi against cognitive impairment in mice via regulation of the fungal microbiota-gut-brain axis[J]. J Agric Food Chem,2022,70(29):9026-9038. doi: 10.1021/acs.jafc.2c03142

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