Volume 45 Issue 5
May  2024
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Article Navigation >  Journal of Kunming Medical University  > 2024  >  45(5): 170-177
Xiaoyue ZHOU, Ting YU. The Application of Narrative Education in the Clinical Teaching of Nursing Interns[J]. Journal of Kunming Medical University, 2024, 45(5): 190-194. doi: 10.12259/j.issn.2095-610X.S20240529
Citation: Fang LUO, Ruibin ZHAO, Guojun LI, Xuxian FU, Xianmou FAN, Shaoming SU, Yunbo WEN, Qilin HUANG, Bin LI. Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method[J]. Journal of Kunming Medical University, 2024, 45(5): 170-177. doi: 10.12259/j.issn.2095-610X.S20240526
shu

Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method

doi: 10.12259/j.issn.2095-610X.S20240526
  • 1.

    Research Institution of Criminal Science and Technology of Kunming Public Security Bureau ，Kunming Yunnan 650500

  • 2.

    College of Forensic Medicine，Kunming Medical University，Kunming Yunnan 650500

  • 3.

    Yunnan Police College，Kunming Yunnan 650221，China

  • Received Date: 2024-01-12
    Available Online: 2024-04-29
  • Publish Date: 2024-05-31
    Abstract
  •   Objective  To study the effect of alcohol on endogenous GHB content in human blood.  Methods  LC-MS/MS inspection method was established in the blood for γ- Hydroxybutyric acid （GHB）, γ- butyrolactone （GBL） and 1,4-butanediol （1,4-BD）. 22 healthy subjects were selected as the research subjects to simulate the real cases of drinking alcohol. The levels of GHB in their blood were detected before and after drinking to compare the endogenous GHB levels in the blood before and after drinking.  Results  GHB in the concentration range of 0.1～50 μg/mL had a good linear relationship and there was a good linear relationship between GBL and 1,4-BD in the concentration range of 0.15～50 μg/mL （R2 > 0.999）.The limits of detection of GHB, GBL and 1,4-BD were 0.015 μg/mL, 0.025 μg/mL and 0.01 μg/mL, respectively, and the limits of GHB, GBL and 1,4-BD quantification were 0.1 μg/mL, 0.15 μg/mL and 0.15 μg/mL. The matrix effects of the three compounds ranged from 18.2% to 20.1%, the recoveries were 81.6% to 92.3%, the intra-day and inter-day precision of RSD were less than 10%, and the accuracy was -5.8%-7.0%. The content of GHB in the blood after 7 hours of drinking were higher than that before drinking alcohol (P < 0.05）, and the concentration range was 0.71-3.81 μg/mL.  Conclusion  The content of endogenous GHB in human blood has increased after 7 hours of drinking alcohol, but the concentration was lower than 4 μg/mL, which can provide the data support for the assessment of endogenous and exogenous GHB involved in real cases, enriching the forensic toxicology data of GHB .
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