Exploring the Effect of TRPC1 and BK-α expression on Diabetic Nephropathy

Hongming LIU; Zhisong CHEN; Lifang ZOU; Zhixiong YANG; Zhuo YU; Wei HU

doi:10.12259/j.issn.2095-610X.S20240603

Volume 45 Issue 6

Jul. 2024

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Hongming LIU, Zhisong CHEN, Lifang ZOU, Zhixiong YANG, Zhuo YU, Wei HU. Exploring the Effect of TRPC1 and BK-α expression on Diabetic Nephropathy[J]. Journal of Kunming Medical University, 2024, 45(6): 15-21. doi: 10.12259/j.issn.2095-610X.S20240603

Citation:

Hongming LIU, Zhisong CHEN, Lifang ZOU, Zhixiong YANG, Zhuo YU, Wei HU. Exploring the Effect of TRPC1 and BK-α expression on Diabetic Nephropathy[J]. Journal of Kunming Medical University, 2024, 45(6): 15-21. doi: 10.12259/j.issn.2095-610X.S20240603

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Exploring the Effect of TRPC1 and BK-α expression on Diabetic Nephropathy

doi: 10.12259/j.issn.2095-610X.S20240603

The 2nd Department of Cardiology，the 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2023-09-23
Available Online: 2024-05-23
Publish Date: 2024-06-25

Abstract

Abstract

Objective To explore the effect of transient receptor potential channel 1 （TRPC1） protein and large conductivity Ca²⁺ activated K⁺ channel α subunit （BK-α） protein on diabetic nephropathy （DKD）. Methods SD rats were randomly divided into a control group （n=15） and a model group （n=15）. Construct a DKD model using high-fat feed and streptozocin （STZ）. Using a blood glucose analyzer to detect changes in blood glucose levels in rats; Using a fully automatic biochemical analyzer to detect the renal function level of rats; HE staining was used to detect pathological changes in renal tissue to confirm successful modeling. Real time fluorescence quantitative PCR （RT-qPCR） and Western blotting were used to detect the mRNA and protein expression levels of TRPC1 and BK-α in renal tissue, respectively; Immunohistochemical detection of the distribution and expression of TRPC1 and BK-α. Results The fasting plasma glucose （FPG）, urinary albumin excretion rate （UAER）, blood urea nitrogen （BUN）, and creatinine （Cr） in the model group were significantly higher than those in the control group （P < 0.01）; the inner wall cells of the renal tubules in the model group showed swelling, with some cells detached; visible renal tubular lesions or death. In addition, neutrophils and their debris were found in many renal tubules and interstitial regions; the above HE staining results indicate that the DKD model has been successfully replicated. TRPC1 and BK-α were most abundant in the glomerulus, and the mRNA and protein levels of TRPC1 and BK-α in the renal tissue of the model group rats were significantly higher than those in the control group （P < 0.05）. Conclusion Diabetic nephropathy affects the distribution and expression of TRPC1 and BK-α in renal tissue.

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References(17)

References

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