Expression of EMC6，C-myc and BCL-2 in Cervical Cancer Tissues and Prognosis Analysis

Chunxia MIAO; Yina WANG; Yunxia ZHANG

doi:10.12259/j.issn.2095-610X.S20240709

Volume 45 Issue 7

Jul. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(7): 62-68

Chunxia MIAO, Yina WANG, Yunxia ZHANG. Expression of EMC6，C-myc and BCL-2 in Cervical Cancer Tissues and Prognosis Analysis[J]. Journal of Kunming Medical University, 2024, 45(7): 62-68. doi: 10.12259/j.issn.2095-610X.S20240709

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Chunxia MIAO, Yina WANG, Yunxia ZHANG. Expression of EMC6，C-myc and BCL-2 in Cervical Cancer Tissues and Prognosis Analysis[J]. Journal of Kunming Medical University, 2024, 45(7): 62-68. doi: 10.12259/j.issn.2095-610X.S20240709

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Expression of EMC6，C-myc and BCL-2 in Cervical Cancer Tissues and Prognosis Analysis

doi: 10.12259/j.issn.2095-610X.S20240709

Dept. of Gynecological Radiation Oncology，The 3rd Clinical Medical College of Xinjiang Medical University，Urumqi Xinjiang 830000，China

Received Date: 2023-12-21
Available Online: 2024-06-14
Publish Date: 2024-07-25

Abstract

Abstract

Objective This study employs bioinformatics analysis to evaluate the expression, immune correlation, and diagnostic value of EMC6 gene in pan-cancer and cervical cancer, and investigate the associations of EMC6, C-myc, and BCL-2 proteins with cervical cancer and patient prognosis. Methods This study analyzed the expression of EMC6 across pan-cancer and cervical cancer datasets from TCGA and GTEx, utilizing SangerBox and Timer databases to assess its correlation with immune response and employing ROC analysis to evaluate its diagnostic potential. EMC6, C-myc, and BCL-2 protein expressions were detected in 68 cases of cervical cancer and adjacent tissues using immunohistochemistry, and their associations with clinicopathological characteristics were analyzed. The Phi coefficient method was employed to assess correlations, and the Kaplan-Meier method was used to evaluate the impact of these proteins on prognosis. Results In cervical cancer, the expression of EMC6 decreased, which significantly affected immune infiltration and had a high diagnostic accuracy （AUC = 0.819）. Immunohistochemistry revealed that compared to the adjacent tissues of cervical cancer, the expression of EMC6 protein decreased, while the expressions of C-myc and BCL-2 proteins were upregulated, with statistically significant differences （P < 0.05）. EMC6 expression showed no correlation with clinicopathological features （P > 0.05）, while C-myc was associated with pathological type （P < 0.05）, and BCL-2 was associated with age, pathological type, tumor size, and degree of differentiation （ P < 0.05）. In cervical cancer, the expression of EMC6 was negatively correlated with C-myc and BCL-2 （Phi = -0.367, P = 0.002; Phi = -0.284, P = 0.019）, and all three were significantly associated with overall patient survival （ P < 0.05）. Conclusions The expression of EMC6 protein decreases in cervical cancer and is negatively correlated with the expression of C-myc and BCL-2 proteins. There is a significant correlation between the expression levels of these proteins and the overall survival of patients, suggesting that they may be used as treatments for cervical cancer and Potential biomarkers for prognostic assessment.
- EMC6,
- C-myc,
- BCL-2,
- Cervical cancer,
- Apoptosis

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