Investigation of Molecular Typing and Drug Resistance of <i>Shigella</i> in Yunnan Province from 2016 to 2022

Yanzhu YIN; Zushun YANG; Yanqiushuo ZOU; Xiang REN; Yidan GUO; Lu FAN

doi:10.12259/j.issn.2095-610X.S20240712

Volume 45 Issue 7

Jul. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(7): 79-86

Yanzhu YIN, Zushun YANG, Yanqiushuo ZOU, Xiang REN, Yidan GUO, Lu FAN. Investigation of Molecular Typing and Drug Resistance of Shigella in Yunnan Province from 2016 to 2022[J]. Journal of Kunming Medical University, 2024, 45(7): 79-86. doi: 10.12259/j.issn.2095-610X.S20240712

Citation:

Yanzhu YIN, Zushun YANG, Yanqiushuo ZOU, Xiang REN, Yidan GUO, Lu FAN. Investigation of Molecular Typing and Drug Resistance of Shigella in Yunnan Province from 2016 to 2022[J]. Journal of Kunming Medical University, 2024, 45(7): 79-86. doi: 10.12259/j.issn.2095-610X.S20240712

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Investigation of Molecular Typing and Drug Resistance of Shigella in Yunnan Province from 2016 to 2022

doi: 10.12259/j.issn.2095-610X.S20240712

1.
Yangbi Center for Disease Control and Prevention，Yangbi Yunnan 672500
2.
Inspection Center，Yunnan Center for Disease Control and Prevention，Kunming Yunnan 650022，China

Received Date: 2024-02-18
Available Online: 2024-06-15
Publish Date: 2024-07-25

Abstract

Abstract

Objective To analyze the serum typing, PFGE molecular typing, and drug-resistance of Shigella strains isolated from the active surveillance for foodborne diseases in Yunnan province from 2016 to 2022, the aim is to understand the pathogen characteristics of Shigella in this period and provide the data support for the control and treatment of Shigella infection. Methods Shigella strains were isolated from the feces of patients between January 2016 and December 2022. Serological typing of the strains was performed using the slide agglutination. Pulsed field gel electrophoresis （PFGE） was employed to detect the molecular typing of the strains. Drug resistance test was achieved by the broth microdilution method. Results A total of 89 Shigella strains were isolated, of which Shigella flexneri accounted for 52.81% and Shigella sonnei 47.19%. Both Shigella flexneri and Shigella sonnei were divided into A and B clusters in PFGE analysis, with B as the predominant cluster. The PFGE bands of 47 Shigella flexneri strains were categorized as 30 types with 3 dominant classes, while 42 Shigella sonnei strains contained 22 types PFGE bands with 4 dominant classes. Two suspected outbreaks were distinguished. Shigella displayed varying degrees of drug resistance to 13 antibiotics, with a 78.65% of multiple resistant strains. Conclusion The dominant PFGE bands of Shigella in Yunnan province were clear, partial of which appeared the clustering distribution. The drug resistance of Shigella was severe and the phenomena of multiple drug resistance were very serious. Drug resistance prototypes between Shigella flexneri and Shigella sonnei were different.
- Shigella,
- Serum typing,
- Pulsed field gel electrophoresis,
- Drug-resistance

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