Polymorphism Investigation of 23 Autosome STR Loci in the Microreader<sup>TM</sup> 23HS Plex ID System in Han Population in Northern China

Yan SHI; Jian JIANG; Chong CHEN; Jingjing ZHANG; Li JIA; Zhixiao GAO; Huifen LI; Jiangwei YAN; He REN

doi:10.12259/j.issn.2095-610X.S20240723

Volume 45 Issue 7

Jul. 2024

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Article Navigation > Journal of Kunming Medical University > 2024 > 45(7): 154-159

Yan SHI, Jian JIANG, Chong CHEN, Jingjing ZHANG, Li JIA, Zhixiao GAO, Huifen LI, Jiangwei YAN, He REN. Polymorphism Investigation of 23 Autosome STR Loci in the MicroreaderTM 23HS Plex ID System in Han Population in Northern China[J]. Journal of Kunming Medical University, 2024, 45(7): 154-159. doi: 10.12259/j.issn.2095-610X.S20240723

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Yan SHI, Jian JIANG, Chong CHEN, Jingjing ZHANG, Li JIA, Zhixiao GAO, Huifen LI, Jiangwei YAN, He REN. Polymorphism Investigation of 23 Autosome STR Loci in the Microreader^TM 23HS Plex ID System in Han Population in Northern China[J]. Journal of Kunming Medical University, 2024, 45(7): 154-159. doi: 10.12259/j.issn.2095-610X.S20240723

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Polymorphism Investigation of 23 Autosome STR Loci in the Microreader^TM 23HS Plex ID System in Han Population in Northern China

doi: 10.12259/j.issn.2095-610X.S20240723

1.
Beijing Tongda Shoucheng Institute of Forensic Science，Beijing 100192
2.
Fujian Nanfang Judicial Expertise Center，Fuzhou Fujian 350000
3.
Beijing Huayan Judicial Authentication Institute，Beijing 100192
4.
Suzhou Microread Gene Technology Co.，LTD，Suzhou Jiangsu 215000
5.
College of Medicine and Forensics，Shanxi Medical University，Jinzhong Shanxi 030600
6.
Dept. of Criminal Science and Technology，Beijing Police College，Beijing 102202，China

Received Date: 2024-03-02
Available Online: 2024-06-14
Publish Date: 2024-07-25

Abstract

Abstract

Objective To obtain the distribution of allele frequency and population genetic data of 23 autosomal STR loci contained in the Microreader^TM 23HS Plex ID System kit in the Han population of northern China, and explore its application value in forensic medicine. Methods The Microreader^TM 23HS Plex ID System kit was used to detect the DNA of 548 unrelated samples which from the Han population in northern China. Then, the allele frequencies, heterozygosity（H） in the samples, polymorphism information content（PIC）, power of discrimination（DP）, and probability of paternity exclusion（PE） were calculated and Hardy-Weinberg equilibrium was tested on each locus based on the genotyping data of 548 samples we collected. The cumulative individual identification ability （CDP） and cumulative non-paternity exclusion rate （CPE）of the Microreader^TM 23HS Plex ID System were also calculated. Results A total of 260 alleles were detected in 548 unrelated samples from 23 STR loci which conformed to Hardy-Weinberg equilibrium. And the alleles frequencies ranged from 0.0009 to 0.5902, H ranged from 0.611 to 0.885, PIC ranged from 0.577 to 0.864, DP ranged from 0.815 to 0.973 （mean DP was 0.922） and PE ranged from 0.089 to 0.406. At the same time, the CDP of Microreader^TM 23HS Plex ID System was 1-3.663×10^-27 and CPE was 1-2.668×10^-16. Conclusion The 23 loci of Microreader^TM 23HS Plex ID System have a good polymorphism in the north Chinese Han population, and have high application value in population genetics research, forensic personal identification, paternity testing and complex kinship identification.
- Forensic genetics,
- Genetic polymorphism,
- STR loci

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References(17)

References

[1]	苏永东,万洪静,陈冲,等. 云南地区汉族群体21个常染色体短串联重复序列基因座遗传多样性及群体遗传学关系[J]. 海南医学,2023,34(19):2749-2754.
[2]	李成涛,刘希玲,孙宏钰,等. SF/T 0117-2021,生物学全同胞关系鉴定技术规范[S]. 中华人民共和国司法部.
[3]	李成涛,孙宏钰,张素华,等. SF/T 0131-2023,生物学半同胞关系鉴定技术规范 [S]. 中华人民共和国司法部.
[4]	谭政,马冠车,付丽红,等. 生物学半同胞关系鉴定策略[J]. 法医学杂志,2023,39(3):262-270.
[5]	宋天福,朱洋,郑冰洁. 浙江汉族人群新常染色体STR基因座的遗传多态性分析[J]. 百科论坛电子杂志,2021(22):2806-2808.
[6]	Shi M,Yu X,Bai R,et al. Genetic polymorphism of 14 non-CODIS STR loci for forensic use in southeast China population[J]. Forensic Science International,2008,174(1):77-80. doi: 10.1016/j.forsciint.2007.02.021
[7]	吴微微, 刘冰, 郝宏蕾, 等. 中国28个省/区汉族人群41个STR基因座多态性数据分析[J]. 中国法医学杂志,2016,31(1):27-32.
[8]	吴谨, 李英碧, 侯一平, 等. 成都汉族群体七个短串联重复序列基因座的遗传多态性和法医学应用研究[J]. 中华医学遗传学杂志,2006,23(2):230-233.
[9]	白雪, 丛斌, 李淑瑾, 等. 分子克隆技术制备miniSTR基因座等位基因分型标准物[J]. 法医学杂志,2009,25(2):106-108.
[10]	曹枝, 滕少康, 黄世宁, 等. 广西壮族D6S477等四个STR基因座的遗传多态性[J]. 解剖科学进展,2007,13(2):134-137.
[11]	梁淑桢,陈俊超. 广东省韶关地区汉族人群20个STR基因座的遗传多态性[J]. 广东医科大学学报,2020,38(02):153-156+159. doi: 10.3969/j.issn.1005-4057.2020.02.006
[12]	候一平. 法医DNA分析的科学证据意义[J]. 中国法医学杂志,2001,16(2):118-120.
[13]	申琴, 杨红旗, 袁朝晖, 等. DNA鉴定技术在法医物证学中的现状和展望[J]. 生物技术世界,2015,10:252-253.
[14]	蔡颖,周广彪,赵书民,等. 中国人群亲权鉴定常用STR基因座平均突变率的估计[J]. 中国司法鉴定,2010,52(05):56-59.
[15]	伍新尧,童大跃,朱运良,等. 用STR分型技术作亲权鉴定时判断标准的研究[J]. 中山大学学报(医学科学版),2010,31(01):1-6.
[16]	李海霞,李建金,李燃,等. 两个已知全同胞参与的同胞鉴定分析[J]. 中国法医学杂志,2019,34(02):159-164.
[17]	Budowle B, Sajantila A. Short tandem repeats-how microsatellites became the currency of forensic genetics[J]. Nat Rev Genet,2024,25(7):450-451. doi: 10.1038/s41576-024-00721-1

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