The Association between ESAT-6 Combined Multiple Detection Indicators and the Severity of Anti-tuberculosis Drug-induced Liver Injury

Nihong LU; Honglu LIU; Yangjun CHEN; Yaping SUN; Yan YANG; Yongrui YANG; Jie LI

doi:10.12259/j.issn.2095-610X.S20240808

Volume 45 Issue 8

Aug. 2024

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Nihong LU, Honglu LIU, Yangjun CHEN, Yaping SUN, Yan YANG, Yongrui YANG, Jie LI. The Association between ESAT-6 Combined Multiple Detection Indicators and the Severity of Anti-tuberculosis Drug-induced Liver Injury[J]. Journal of Kunming Medical University, 2024, 45(8): 52-57. doi: 10.12259/j.issn.2095-610X.S20240808

Citation:

Nihong LU, Honglu LIU, Yangjun CHEN, Yaping SUN, Yan YANG, Yongrui YANG, Jie LI. The Association between ESAT-6 Combined Multiple Detection Indicators and the Severity of Anti-tuberculosis Drug-induced Liver Injury[J]. Journal of Kunming Medical University, 2024, 45(8): 52-57. doi: 10.12259/j.issn.2095-610X.S20240808

Citation:

Nihong LU, Honglu LIU, Yangjun CHEN, Yaping SUN, Yan YANG, Yongrui YANG, Jie LI. The Association between ESAT-6 Combined Multiple Detection Indicators and the Severity of Anti-tuberculosis Drug-induced Liver Injury[J]. Journal of Kunming Medical University, 2024, 45(8): 52-57. doi: 10.12259/j.issn.2095-610X.S20240808

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The Association between ESAT-6 Combined Multiple Detection Indicators and the Severity of Anti-tuberculosis Drug-induced Liver Injury

doi: 10.12259/j.issn.2095-610X.S20240808

The 3rd People’s Hospital of Kunming，Yunnan Provincial Clinical Medical Center for Infectious Diseases，Kunming Yunnan 650041，China

Received Date: 2023-12-29
Available Online: 2024-07-06
Publish Date: 2024-08-05

Abstract

Abstract

Objective To detect the expression level of ESAT-6 combined with IL-6, IL-10, IFN-γ, CK-18, MMP-7, MMP-9 in the serum of patients with anti-tuberculosis drug induced liver injury （ATB-DILI）, to analyze the correlation between the above indicators and the severity of liver injury, and to explore the evaluation value of 7 factors for ATB-DILI. Methods From January 2019 to January 2023, pulmonary tuberculosis patients treated at the Third People's Hospital of Kunming were included. Using the ELISA method, the levels of ESAT-6, IL-6, IL-10, IFN-γ, CK-18, MMP-7, and MMP-9 in the serum of 210 ATB-DILI tuberculosis patients （Group A）, 120 tuberculosis patients without liver damage during anti-tuberculosis treatment （Group B）, and 50 healthy individuals undergoing physical examinations （Group C） were tested. The correlation between the above indicators and the severity of liver damage was analyzed, as well as the accuracy of assessing the severity of liver damage. Results The age of patients in group A was higher than that in group B and C （P < 0.05）. Compared with the control group, the expression of serum ESAT-6, IL-10, CK-18, MMP-7 and MMP-9 was significantly up-regulated in group A, and the expression of ESAT-6 and MMP-7 was significantly up-regulated in group B （P < 0.05）. The expression levels of CK-18, IL-10, and MMP-9 in Group A were higher than in Group B （P < 0.05）. In Group A, age, ESAT-6, CK-18, and MMP-9 were positively correlated with the severity of liver injury （P < 0.05）. Logistic regression analysis indicated that age >65 years, ESAT-6 >31.3 pg/mL, and CK-18 >45.5 ng/mL in Group A patients were independent risk factors for ATB-DILI. Conclusion Elderly patients are more prone to ATB-DILI. Elevated levels of ESAT-6, CK-18, and MMP-9 are related to the severity of ATB-DILI. Age over 65, ESAT-6 > 31.3 pg/mL, and CK-18 > 45.5 ng/mL are independent risk factors for ATB-DILI. The combined detection of ESAT-6 and CK-18 expression levels in elderly patients holds significant clinical value for predicting and assessing the severity of ATB-DILI.
- ESAT-6,
- CK-18,
- ATB-DILI,
- IL,
- MMP

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