Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs

Long PU; Xuanran ZHOU; Chenrong JIANG; Yunxuan LI; Yong YUAN

doi:10.12259/j.issn.2095-610X.S20240903

Volume 45 Issue 9

Sep. 2024

Turn off MathJax

Article Contents

Article Navigation > Journal of Kunming Medical University > 2024 > 45(9): 17-23

Long PU, Xuanran ZHOU, Chenrong JIANG, Yunxuan LI, Yong YUAN. Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs[J]. Journal of Kunming Medical University, 2024, 45(9): 17-23. doi: 10.12259/j.issn.2095-610X.S20240903

Citation:

Long PU, Xuanran ZHOU, Chenrong JIANG, Yunxuan LI, Yong YUAN. Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs[J]. Journal of Kunming Medical University, 2024, 45(9): 17-23. doi: 10.12259/j.issn.2095-610X.S20240903

Citation:

PDF( 1291 KB)

Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs

doi: 10.12259/j.issn.2095-610X.S20240903

1.
Dept. of Trauma Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
Dept. of Orthopedic Joint Sports Trauma，Mengzi City People’s Hospital，Mengzi Yunnan 661100，China

Received Date: 2024-03-20
Available Online: 2024-06-14
Publish Date: 2024-09-25

Abstract

Abstract

Objective To investigate the impact of inhibiting peroxisome proliferator-activated receptor γ （PPARγ） expression on osteogenic differentiation of BMSCs, so as to provide a theoretical basis for the treatment of primary osteoporosis （POP）. Methods BMSCs were extracted from patients with normal bone density and categorized into the normal control group（CON group）. BMSCs extracted from patients with primary osteoporosis were categorized into the primary osteoporosis group （POP group）. The cells from the POP group were treated with a PPARγ inhibitor and divided into the inhibitor group （INR group）. Following osteogenic differentiation, the expression of osteopontin （OPN）, osteocalcin （OCN）, Osterix, and runt-related transcription factor 2 （Runx2） was evaluated in the cells of each group.The alkaline phosphatase （ALP） staining was employed to assess the osteogenic differentiation of BMSCs in each group. Results The quantity of ALP-positive cells in the POP group was lower than that in the CON and INR groups （P < 0.05）. The expression of OCN, OPN, Osterix, and Runx2 was found to be decreased in the POP group when compared to the CON group, with statistically significant differences (P < 0.05）. Conversely, the expression of OCN, OPN, Osterix, and Runx2 was elevated in the INR group when compared to the POP group, with statistically significant differences (P < 0.05）. Conclusion Increased expression of osteogenic differentiation-specific genes （ALP, OCN, OPN, Osterix, Runx2） in BMSCs after inhibition of PPARγ expression.
- POP,
- PPARγ,
- BMSCs,
- Osteogenic differentiation

FullText(HTML)

References(17)

References

[1]	中华医学会骨质疏松和骨矿盐疾病分会. 原发性骨质疏松症诊疗指南(2022)[J]. 中国全科医学,2023,26(14):1671-1691.
[2]	Muruganandan S,Ionescu A M,Sinal C J. At the crossroads of the adipocyte and osteoclast differentiation programs: Future therapeutic perspectives[J]. International Journal of Molecular Sciences,2020,21(7):2277. doi: 10.3390/ijms21072277
[3]	Wu H,Li X,Shen C. Peroxisome proliferator-activated receptor gamma in white and brown adipocyte regulation and differentiation[J]. Physiological Research,2020,69(5):759-773.
[4]	Vita F,Gangemi S,Pioggia G,et al. Physical activity and post-transcriptional regulation of aging decay: Modulation of pathways in postmenopausal osteoporosis[J]. Medicina-Lithuania,2022,58(6):767.
[5]	Li X,Ning L,Ma J,et al. The PPAR-γ antagonist T007 inhibits RANKL-induced osteoclastogenesis and counteracts OVX-induced bone loss in mice[J]. Cell Communication and Signaling: CCS,2019,17(1):136. doi: 10.1186/s12964-019-0442-3
[6]	Lee M J. Hormonal regulation of adipogenesis[J]. Comprehensive Physiology,2017,7(4):1151-1195.
[7]	Yu B,Wang CY. Osteoporosis and periodontal diseases - an update on their association and mechanistic links[J]. Periodontology,2000,2022,89(1):99-113.
[8]	Agidigbi T S,Kim C. Reactive oxygen species in osteoclast differentiation and possible pharmaceutical targets of ros-mediated osteoclast diseases[J]. International Journal of Molecular Sciences,2019,20(14):3576. doi: 10.3390/ijms20143576
[9]	Domazetovic V,Marcucci G,Iantomasi T,et al. Oxidative stress in bone remodeling: role of antioxidants[J]. Clinical Cases in Mineral and Bone Metabolism,2017,14(2):209-216. doi: 10.11138/ccmbm/2017.14.1.209
[10]	Zhou Q,Zhu L,Zhang D,et al. Oxidative stress-related biomarkers in postmenopausal osteoporosis: A systematic review and meta-analyses[J]. Disease Markers,2016,2016:7067984.
[11]	Abdallah B M. Marrow adipocytes inhibit the differentiation of mesenchymal stem cells into osteoblasts via suppressing bmp-signaling[J]. Journal of Biomedical Science,2017,24(1):11-11. doi: 10.1186/s12929-017-0321-4
[12]	Chandra A,Rajawat J. Skeletal aging and osteoporosis: Mechanisms and therapeutics[J]. International Journal of Molecular Sciences,2021,22(7):3553. doi: 10.3390/ijms22073553
[13]	Ahmadian M,Suh J M,Hah N,et al. PPARγ signaling and metabolism: the good,the bad and the future[J]. Nature Medicine,2013,19(5):557-566. doi: 10.1038/nm.3159
[14]	Cao J,Ou G,Yang N,et al. Impact of targeted PPARγ disruption on bone remodeling[J]. Mol Cell Endocrinol,2015,410:27-34. doi: 10.1016/j.mce.2015.01.045
[15]	Albers J,Keller J,Baranowsky A,et al. Canonical wnt signaling inhibits osteoclastogenesis independent of osteoprotegerin[J]. The Journal of Cell Biology,2013,200(4):537-549. doi: 10.1083/jcb.201207142
[16]	Li Y,Yao L,Lu J. IL-35 inhibits adipogenesis via PPARγ-wnt/β-catenin signaling pathway by targeting axin2[J]. Int Immunopharmacol,2023,122:110615. doi: 10.1016/j.intimp.2023.110615
[17]	Komori T. Regulation of proliferation,differentiation and functions of osteoblasts by runx2[J]. Int J Mol Sci,2019,20(7):1694. doi: 10.3390/ijms20071694

Relative Articles(20)

Relative Articles

[1]	Shibing QIAN, Huiping SHI, Yanqiu LI, Rongyu YANG, Kaiwen DUAN. Research Progress on Osteogenic Differentiation of Apical Papilla Stem Cells. Journal of Kunming Medical University, 2024, 45(9): 168-173. doi: 10.12259/j.issn.2095-610X.S20240926
[2]	Shibing QIAN, Lingpeng ZHANG, Lingyun YIN, Changquan LI, Hu LI, Hongbin YU. Study on Osteogenic Ability of Dental Pulp Stem Cells at Different Concentrations. Journal of Kunming Medical University, 2023, 44(2): 61-68. doi: 10.12259/j.issn.2095-610X.S20230214
[3]	Qian XU, Yumei CUI, Siming MA, Yunhong LIN, Yijing XIONG, Zijun SONG, Xudong LI. miR-148a-3p Targeting SMURF2 in Regulating Osteogenic Differentiation and Enamel Development during In Vitro Tooth Organogenesis. Journal of Kunming Medical University, 2023, 44(11): 16-21. doi: 10.12259/j.issn.2095-610X.S20231103
[4]	Boyi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cuijuan MIAO, Cheng ZHANG, Zhujun HAN. The Relationship Between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis. Journal of Kunming Medical University, 2022, 43(3): 67-73. doi: 10.12259/j.issn.2095-610X.S20220306
[5]	Bo-yi LI, Ling NIU, Rong MA, Xian ZHANG, Fang LIU, Yan TANG, Cui-juan MIAO, Zhu-jun HAN, Cheng ZHANG. The Relationship between T950C Polymorphism in the Promoter Region of Osteoprotegerin Gene and Type 2 Diabetes with Osteoporosis in Kunming Area. Journal of Kunming Medical University, 2021, 43(): 1-8. doi: 10.12259/j.issn.2095-610X.S20211112
[6]	Ling-ling YAN, Zhi-hong QIAN, Yue NI, Huang WU. Clinical Investigation of Vitamin D and Calcium Supplement’ Effect on the Occurrence of Cardiovascular and Cerebrovascular Diseases in POP Patients. Journal of Kunming Medical University, 2021, 42(10): 139-144. doi: 10.12259/j.issn.2095-610X.S20211036
[7]	Liu Chong , Cao Hui , Yang Cai Cai , Wang Zhen . . Journal of Kunming Medical University, 2020, 41(06): 32-38.
[8]	Zhang Ying , Lu Yu , Zhao Zhi , Li Tao , Bi Ni , Shi Zhi Yue , Wang Ying Song , Jie Jing Ming , Song Yue Ming , Chang Li Tao , Guan Shuai , Tang Lin Bo . . Journal of Kunming Medical University, 2019, 40(11): 63-68.
[9]	Zhao Xian , Han Xue Song , Zhou Qing Zhu , Li Shan , Liu Liu , Wang Song Mei . The Bone Defect Repairment in Vivo with Co-cultured EPCs and BMSCs. Journal of Kunming Medical University, 2018, 39(03): 11-18.
[10]	Ji Qin Long , Kong Xiang Dong , Qi Shan Hong , Li Chao , Fan Jun , Sha Yong , Wang Shao Feng . The Experimental Study of Cyasterone on the Treatment of Osteoporosis through the Bidirectional Effect of Inhibiting Osteoclast Differetiation and Promoting Osteoblast Differentiation. Journal of Kunming Medical University, 2018, 39(05): 21-28.
[11]	Ye Lei , Tong Fa Chun , Li Jian , Wang Long , Zhao Jiang Ming , Liu Yi , Wang Jian . The Regulatory Effect of PPARy in the Apoptosis against Renal Cell Carcinoma. Journal of Kunming Medical University, 2018, 39(05): 29-34.
[12]	Yang Zai Ying . The Effect of Osteoporosis on the Pathogenesis of Osteoarthritis. Journal of Kunming Medical University,
[13]	Hu Zheng Xiong . Effect of TGF-β2 and geneX on the Proliferation and Osteogenic Differentiation of BrdU-labeled Bone Mesenchymal Stem Cells. Journal of Kunming Medical University,
[14]	Liu Jun Ping . . Journal of Kunming Medical University,
[15]	Yao Rong Cheng . . Journal of Kunming Medical University,
[16]	Zhao Gang . . Journal of Kunming Medical University, 2015, 36(01): 1-1.
[17]	Yao Han Xi . . Journal of Kunming Medical University,
[18]	Yu Le . . Journal of Kunming Medical University,
[19]	Wu Bin . . Journal of Kunming Medical University,
[20]	. The Clinical Analysis of Cushing's Syndrome-Related Osteoporosis. Journal of Kunming Medical University,