Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs

Long PU; Xuanran ZHOU; Chenrong JIANG; Yunxuan LI; Yong YUAN

doi:10.12259/j.issn.2095-610X.S20240903

Volume 45 Issue 9

Sep. 2024

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Long PU, Xuanran ZHOU, Chenrong JIANG, Yunxuan LI, Yong YUAN. Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs[J]. Journal of Kunming Medical University, 2024, 45(9): 17-23. doi: 10.12259/j.issn.2095-610X.S20240903

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Long PU, Xuanran ZHOU, Chenrong JIANG, Yunxuan LI, Yong YUAN. Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs[J]. Journal of Kunming Medical University, 2024, 45(9): 17-23. doi: 10.12259/j.issn.2095-610X.S20240903

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Effect of Inhibiting PPARγ Expression on Osteogenic Differentiation of BMSCs

doi: 10.12259/j.issn.2095-610X.S20240903

1.
Dept. of Trauma Surgery，The 2nd Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
Dept. of Orthopedic Joint Sports Trauma，Mengzi City People’s Hospital，Mengzi Yunnan 661100，China

Received Date: 2024-03-20
Available Online: 2024-06-14
Publish Date: 2024-09-25

Abstract

Abstract

Objective To investigate the impact of inhibiting peroxisome proliferator-activated receptor γ （PPARγ） expression on osteogenic differentiation of BMSCs, so as to provide a theoretical basis for the treatment of primary osteoporosis （POP）. Methods BMSCs were extracted from patients with normal bone density and categorized into the normal control group（CON group）. BMSCs extracted from patients with primary osteoporosis were categorized into the primary osteoporosis group （POP group）. The cells from the POP group were treated with a PPARγ inhibitor and divided into the inhibitor group （INR group）. Following osteogenic differentiation, the expression of osteopontin （OPN）, osteocalcin （OCN）, Osterix, and runt-related transcription factor 2 （Runx2） was evaluated in the cells of each group.The alkaline phosphatase （ALP） staining was employed to assess the osteogenic differentiation of BMSCs in each group. Results The quantity of ALP-positive cells in the POP group was lower than that in the CON and INR groups （P < 0.05）. The expression of OCN, OPN, Osterix, and Runx2 was found to be decreased in the POP group when compared to the CON group, with statistically significant differences (P < 0.05）. Conversely, the expression of OCN, OPN, Osterix, and Runx2 was elevated in the INR group when compared to the POP group, with statistically significant differences (P < 0.05）. Conclusion Increased expression of osteogenic differentiation-specific genes （ALP, OCN, OPN, Osterix, Runx2） in BMSCs after inhibition of PPARγ expression.
- POP,
- PPARγ,
- BMSCs,
- Osteogenic differentiation

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References(17)

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