Analysis of Laboratory Index Characteristics in Newly Treated Smear-Negative Pulmonary Tuberculosis Patients

Xinye YANG; Nian ZHANG; Sha MA; Linpei HUANG; Xueyi LI; Xiaoping HUANG; Mi ZHANG; Cuixian YANG; Xicheng WANG

doi:10.12259/j.issn.2095-610X.S20240909

Volume 45 Issue 9

Sep. 2024

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Xinye YANG, Nian ZHANG, Sha MA, Linpei HUANG, Xueyi LI, Xiaoping HUANG, Mi ZHANG, Cuixian YANG, Xicheng WANG. Analysis of Laboratory Index Characteristics in Newly Treated Smear-Negative Pulmonary Tuberculosis Patients[J]. Journal of Kunming Medical University, 2024, 45(9): 56-61. doi: 10.12259/j.issn.2095-610X.S20240909

Citation:

Xinye YANG, Nian ZHANG, Sha MA, Linpei HUANG, Xueyi LI, Xiaoping HUANG, Mi ZHANG, Cuixian YANG, Xicheng WANG. Analysis of Laboratory Index Characteristics in Newly Treated Smear-Negative Pulmonary Tuberculosis Patients[J]. Journal of Kunming Medical University, 2024, 45(9): 56-61. doi: 10.12259/j.issn.2095-610X.S20240909

Citation:

Xinye YANG, Nian ZHANG, Sha MA, Linpei HUANG, Xueyi LI, Xiaoping HUANG, Mi ZHANG, Cuixian YANG, Xicheng WANG. Analysis of Laboratory Index Characteristics in Newly Treated Smear-Negative Pulmonary Tuberculosis Patients[J]. Journal of Kunming Medical University, 2024, 45(9): 56-61. doi: 10.12259/j.issn.2095-610X.S20240909

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Analysis of Laboratory Index Characteristics in Newly Treated Smear-Negative Pulmonary Tuberculosis Patients

doi: 10.12259/j.issn.2095-610X.S20240909

Xinye YANG^{1, 2},
Nian ZHANG¹,
Sha MA¹,
Linpei HUANG^{1, 2},
Xueyi LI^{1, 3},
Xiaoping HUANG^{1, 2},
Mi ZHANG¹,
Cuixian YANG^{1
,
,},
Xicheng WANG^{1
,
,}

1.
Yunnan Provincial Hospital of Infectious Diseases，Kunming Yunnan 650301
2.
School of Public Health，Kunming Medical University，Kunming Yunnan 650500
3.
School of Public Health，Dali University，Dali Yunnan 671003，China

Received Date: 2023-12-15
Available Online: 2024-09-01
Publish Date: 2024-09-25

Abstract

Abstract

Objective To explore the differences in laboratory test index characteristics among smear-negative pulmonary tuberculosis patients, smear-positive pulmonary tuberculosis patients and healthy people. Methods Fifty patients with initial treatment of pulmonary tuberculosis in our hospital from March 2023 to September 2023 were selected and divided into a smear-negative group and a smear-positive group according to the diagnosis. The general data of the subjects were collected, and the lymphocyte subsets and cytokines levels of the subjects were detected by flow cytometry. The lymphocyte subsets, cytokines, blood routine indexes and biochemical indexes were compared among the groups. Results The proportions of smoking history, drug-resistant tuberculosis, positive sputum culture, positive GeneXpert and pulmonary cavity in the smear-negative group were significantly lower than those in the smear-positive group （P < 0.05）. The counts of CD45⁺, CD3⁺T, CD4⁺T, CD8⁺T and NK cells in PTB patients with negative smears were significantly lower than those in healthy controls （P < 0.05）. The counts of CD45⁺, CD3⁺, CD4⁺, CD8⁺T and B cells were significantly higher than those of smear-positive PTB patients （P < 0.05）. The levels of IL-1β, IL-6, IL-8, IL-10, IL-17 and IFN-γ in the smear-negative PTB group were significantly higher than those in the healthy control group, while the levels of IL-12 and IFN-α were significantly lower than those in the healthy control group （P < 0.05）. The levels of IL-2, IL-5, IL-6, IL-12, IL-17 and IFN-γ in PTB patients in the smear-negative group were significantly lower than those in the smear-positive group （P < 0.05）. The neutrophil ratio, CRP and procalcitonin levels of PTB patients in the smear-negative group were significantly higher than those in the healthy control group （P < 0.05）, and the red blood cell, hemoglobin, total protein, albumin, white blood cell ratio and prealbumin levels were significantly lower than those in the healthy control group （P < 0.05）. The level and proportion of monocytes in PTB patients in the smear-negative group were significantly lower than those in the smear-positive group （P < 0.05）. Conclusion Pulmonary tuberculosis patients have impaired immune function. Compared with smear-negative pulmonary tuberculosis patients, the lymphocyte subsets of sputum smear-positive pulmonary tuberculosis patients are more severely damaged, the levels of cytokines are higher, and the degree of infection is more serious.
- Smeared negative pulmonary tuberculosis,
- Lymphocyte subsets,
- Cytokines,
- Blood routine indicators,
- Biochemical indicators

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References(19)

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