Effect of Compound Probiotic Powder on Immune Function of Mice

Shuaiqiang WANG; Kejin TONG; Shuofeng ZHANG

doi:10.12259/j.issn.2095-610X.S20241005

Volume 45 Issue 10

Oct. 2024

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Shuaiqiang WANG, Kejin TONG, Shuofeng ZHANG. Effect of Compound Probiotic Powder on Immune Function of Mice[J]. Journal of Kunming Medical University, 2024, 45(10): 29-35. doi: 10.12259/j.issn.2095-610X.S20241005

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Shuaiqiang WANG, Kejin TONG, Shuofeng ZHANG. Effect of Compound Probiotic Powder on Immune Function of Mice[J]. Journal of Kunming Medical University, 2024, 45(10): 29-35. doi: 10.12259/j.issn.2095-610X.S20241005

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Effect of Compound Probiotic Powder on Immune Function of Mice

doi: 10.12259/j.issn.2095-610X.S20241005

1.
School of Chinese Herbal Medicine，Beijing University of Chinese Medicine
2.
School of Chinese Medicine Beijing University of Chinese Medicine，Beijing 100105，China

Received Date: 2024-05-22
Available Online: 2024-10-15
Publish Date: 2024-10-31

Abstract

Abstract

Objective To explore the effect of compound probiotic powder on enhancing related immune function of organism, so as to provide theoretical basis and experimental support for further exploration and application of compound probiotic powder in the future. Methods 160 ICR mice were randomly divided into control group, low, medium and high dose groups, and divided into groups by random parallel control grouping method, with 40 mice in each group. The experimental period lasts 30 days, the control group was given normal saline for the same time, and the other groups were given compound probiotic powder for 30 days. After the gavage, the mice were killed alive, and their organ index, delayed allergic reaction, lymphocyte transformation, serum hemolysin, antibody-producing cells, NK cell activity and macrophage phagocytosis were observed to evaluate the influence of compound probiotic powder on the immunity of mice. Results Compared with the control group, there was no significant difference in body weight and organ index of mice in each dose group （F = 0.885, P = 0.458; F = 0.844, P = 0.479; F = 0.732, P = 0.540）; The difference of toe swelling degree was statistically significant （F = 3.252, P = 0.03）.The difference of optical density of spleen lymphocyte proliferation in mice with different doses increased, and the difference was statistically significant （F = 48.786, P = 0.000）. The number of hemolytic plaque increased with statistical significance （F = 22.095, P = 0.000）. The half hemolysis value of middle and high dose groups increased, and the difference was statistically significant （F = 30.544, P = 0.000）. The clearance index and phagocytosis index increased, and the difference was statistically significant and dose-dependent （F = 338.79, P = 0.000; F = 120.271, P = 0.000）; NK cell activity also increased, and the difference was statistically significant and dose-dependent （F = 466.491, P = 0.000）. Conclusion The compound probiotic powder can improve the immunity of mice, and the results meet the evaluation standard of "having the function of enhancing immunity" in the Technical Specification for Inspection and Evaluation of Health Food.
- Lactobacillus rhamnosus R9639,
- Lactobacillus casei zhang,
- Lactobacillus plantarum P9,
- Humoral immunity,
- Cytoimmunity

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References(21)

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