Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis

Aheng WANG; Zhen SONG; Guanghua YANG; Haiyan HE

doi:10.12259/j.issn.2095-610X.S20241107

Volume 45 Issue 11

Nov. 2024

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Aheng WANG, Zhen SONG, Guanghua YANG, Haiyan HE. Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis[J]. Journal of Kunming Medical University, 2024, 45(11): 46-51. doi: 10.12259/j.issn.2095-610X.S20241107

Citation:

Aheng WANG, Zhen SONG, Guanghua YANG, Haiyan HE. Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis[J]. Journal of Kunming Medical University, 2024, 45(11): 46-51. doi: 10.12259/j.issn.2095-610X.S20241107

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Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis

doi: 10.12259/j.issn.2095-610X.S20241107

1.
Dept. of Head and Neck Surgery
2.
Dept. of Outpatient，Qingdao Central Hospital of Rehabilitation University，Qingdao Shandong 266000，China

Received Date: 2024-06-10
Available Online: 2024-10-23
Publish Date: 2024-11-25

Abstract

Abstract

Objective To explore the regulatory mechanism of TLR4/NF-κB/MUC5B in allergic rhinitis. Methods A mouse model of allergic rhinitis was established to observe the changes in pathological characteristics of the model and to detect the expression of TLR4 and MUC5B. By reducing the expression of TLR4 by intranasal administration, the histopathological changes of TLR4, the expression of MUC5B and the concentration of inflammatory factors were observed. An in vitro cell model was established to detect the expression of MUC5B as well as to observe the changes of NF-κB and phosphorylated NF-κB （p-NF-κB） after knockdown of TLR4 by cell transfection. Results （1） TLR4 and MUC5B were highly expressed in a mouse model of allergic rhinitis （P < 0.05）; （2） Lowering the expression of TLR4 could reduce the expression of MUC5B and inflammatory factors, as well as alleviated allergic rhinitis （P < 0.05）; （3） In vitro, knockdown of TLR4 could reduce the expression of p-NF-κB and MUC5B （P < 0.05）. Conclusion The regulatory mechanism of TLR4/NF-κB/ MUC5B in allergic rhinitis provides the new ideas for the treatment of allergic rhinitis.
- Allergic rhinitis,
- TLR4,
- MUC5B,
- NF-κB

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References(29)

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