Effect of Tropisetron and Ondansetron on Postoperative Nausea and Vomiting in Patients Undergoing Abdominal Hysterectomy under General Anesthesia[J]. Journal of Kunming Medical University, 2011, 32(06).
Citation: Culture and Identification of Hippocampus Neurons in Adult Rats[J]. Journal of Kunming Medical University, 2011, 32(06).

Culture and Identification of Hippocampus Neurons in Adult Rats

  • Objective To observe the morphology and characteristics of hippocampus neurons in adult rats in vitro.Method The hippocampus tissues from adult rats were collected,then digested into cell suspension by using 0.125% trypsin.Cell suspension was planted into wells and observed at day 1,3,7,10,13,and 15 after incubation.Neurons specific Enolase(NSE)antibody,recognized specifically NSE antigen in cultured cells,was used to identify neurons by SP-two-step staining method.Results During 1-3 days,the extensive bodi...
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