Expression of the Recombinant Adenovirus Carrying Enhanced Green Fluorescent Protein（EGFP）in Brain and Lung Tissues of Rats

［Abstract］Objective　To investigate the expression of the recombinant adenovirus carrying the enhanced green fluorescent protein（EGFP）in rats' brain and lung by nasal administration，so as to provide a basis for targeting tvansport of adenovirus by intranasal administration．Methods　12 rats were randomly divided into 3 groups（n = 4），rats in 3 groups were respectively given instillation of EGFP recombinant adenovirus fluid， EGFP recombinant adenovirus added by viscous fiber protein solution and control group instillation of saline by nasal administration. Rats in the adenovirus fluid group were given instillation of 20 μL adenovirus fluid from each nostril．Rats in the adenovirus added by viscous fiber protein liquid group were given instillation of 40 μL adenovirus added by viscous fiber protein liquid each nostril. Rats in the control group were given instillation of 20 μL saline．The administration was once a day and lasted for 7 days．Rats were given heart perfusion on the 9th day，and lung and brain tissues were collected for frozen section，and the fluorescence signals in frozen sections were observed by confocal fluorescence microscopy．Results　We observed the expression of green fluorescent protein in the lung tissues of rats in the two treatment groups，but no green fluorescent expression in brain tissues. Conclusion Adenovirus can target rats' lungs by intranasal administration，so it can be used for the carrier of pulmonary targeted drug delivery system.