Correlation Between NO/iNOS and Destruction of Articular Cartilage

Tang Tao; Sun Xian Run; Jiang Yan Ping; Zhang Min; Ma Hua; Long Yu

Article Contents

Article Navigation > Journal of Kunming Medical University > 2016 > 37(11): 28-32

Tang Tao , Sun Xian Run , Jiang Yan Ping , Zhang Min , Ma Hua , Long Yu . Correlation Between NO/iNOS and Destruction of Articular Cartilage[J]. Journal of Kunming Medical University, 2016, 37(11): 28-32.

Citation:

Tang Tao , Sun Xian Run , Jiang Yan Ping , Zhang Min , Ma Hua , Long Yu . Correlation Between NO/iNOS and Destruction of Articular Cartilage[J]. Journal of Kunming Medical University, 2016, 37(11): 28-32.

Citation:

PDF( 270 KB)

Correlation Between NO/iNOS and Destruction of Articular Cartilage

Tang Tao ^1,2,
Sun Xian Run ^1,3,
Jiang Yan Ping ⁴,
Zhang Min ¹,
Ma Hua ¹,
Long Yu ¹

Funds:

基金：云南省应用基础研究(昆医联合专项)基金资助项目(2015FB095); 云南省应用基础研究计划项目(2013FZ182);

Received Date: 2016-05-24

Abstract

Abstract

Objective To investigate the dynamic changes of articular cartilage destruction and nitric oxide nitric oxide/inducible nitric oxide synthase(NO/i NOS). Methods Forty healthy adult male Wistar rats were randomly divided into control group(free movement) and experimental group(overtraining). Total knee joints of rat were collected at week 0,1,2,3,and 6,fixed with 4% PFA,embedded in paraffin, sectioned and stained. Cartilage morphological changes were observed by Safranin-O staining, NO/i NOS content changes in serum and synovial fluid were determined by ELISA,Mankin's scores were obtained, and cartilage pathological changes were analyzed. Results Overtraining resulted in cell sorting disorders, loss of matrix, destruction like rough surface and cracks, and degeneration of cartilage pathology process. Levels of NO/i NOS in serum and synovial fluid increased after overtraining:week 1(48.088±1.534),(3.058±0.073);week 2(54.574±0.800),(6.630±0.524);week 3(68.020±3.184),(7.384±0.306);weeks 6(104.394±8.094),(11.364±0.882).Levels of NO/i NOS in synovial fluid group were: week 1(65.814 ±1.610),(8.126 ±0.724); week 2(72.766±2.344),(9.694±0.564);week 3(88.816±0.819),(11.326±1.473);week 6(120.204±12.044),(15.166±1.198),and high level of concentration was found with the increasing amount of exercise.Conclusion There is a positive correlation between the levels of NO/i NOS in synovial fluid or serum and the extent of the damage of articular cartilage.
- Nitric Oxide,
- Induced Nitricoxide Synthase,
- Cartilage,
- Osteoarthritis

FullText(HTML)

References(32)

References

[1]	[1]SALEH K J,DAVIS A.Measures for Pain and Function Assessments for Patients With Osteoarthritis[J].J Am Acad Orthop Surg,2016,24(11):e148-e162.
[2]	[2]SCANZELLO C R.Role of low-grade inflammation in osteoarthritis[J].Curr Opin Rheumatol(Epub ahead of print DOI:10.1097/BOR.0000000000000353).
[3]梁永生,李芳祥.备战第28届奥运会中国优秀运动员运动损伤调查分析[J].中国运动医学杂志,2007,11,26(6):744-745.
[4]	[4]FELSON D T.Obesity and vocational and avocational overload of the joint as risk factors for osteoarthritis[J].JRheumatol Suppl,2004,70(2):2-5.
[5]邓紫玉,孙鹥,林俊,等.ADAMTS-4在膝骨关节炎关节液中的表达和意义[J].昆明医学院学报,2011,32(6):59-61.
[6]张晓磬,张凤兰,万崇华,等.慢性病患者生命质量测定量表体系之骨关节炎量表的研制及考评[J].昆明医科大学学报,2013,34(8):23-27.
[7]	[7] TANG T,MUNETA T,SEKIYA I.Fibrous change of the infrapatellar fat pad due to strenuous running exercise and its treatment with intraarticular hyaluronan injection in rat mode[lJ].J Med Sci,2008,55(1):163-173.
[8]	[8]PANDOL S J,PERISKIC S,GUKOVSKY I,et al.Ethanol diet increases the sensitivity of rats to pancreatitis induced by cholecystokinin octapeptide[J].Gastroenterology,1999,117(3):706-716.
[9]	[9]NAH S S,CHOI I Y,LEE C K,et al.Effects of advanced glycation end products on the expression of COX-2,PGE2and NO in human osteoarthritic chondrocytes[J].Rheumatology(Oxford),2008,47(4):425-431.
[10]	[10] TANG T,MUNETA T,JU Y J,et al.Serum keratan sulfate transiently increases in early stage of osteoarthritis during strenuous running of rats:Protective effect of intraarticular hyaluronan injection[J].Arthritis Res Ther,2008,10(1)1-8.
[11]	[11]MANKIN H J,DORFMAN H,LIPPIELLO L,et al.Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips.Ⅱ.Correlation of morphology with biochemical and metabolic data[J].J Bone Joint Surg Am,1971,53(3):523-537.
[12]	[12]PAP G,EBERHARDT R,STRMER I,et al.Developent of osteoarthritis in the knee joints of Wistar rats strenuous running exercise in a running wheel by intracranial self-stimulation[J].Pathol Res Pract,1998,194(1):41-47.
[13]	[13]BURGHARDT P R,FULK L J,HAND G A,et al.The effects of chronic treadmill and wheel running on behavior in rats[J].Brain Res,2004,1019(1-2):84-96.
[14]	[14] SEKIYA I,TANG T,HAYASHI M,et al.Periodic knee injections of BMP-7 delay cartilage degeneration induced by excessive running in rats[J].J Orthop Res,2009,27(8):1088-1092.
[15]	[15]SASAKI K,HATTORI T,FUJISAWA T,et al.Nitric oxide mediates interleukin-1-induced gene expression of matrix metalloproteinases and basic fibroblast growth factor in cultured rabbit articular chondrocytes[J].J Biochem,1998,123(3):431-439.
[16]	[16]CLANCY R M,REDISKE J,TANG X,et al.Outside-in signaling in the chondrocyte.Nitric oxide disrupts fibronectin-induced assembly of a subplasmalemmal actin/rho A/focal adhesion kinase signaling complex[J].JClin Invest,1997,100(7):1789-1796.
[17]	[17]TAMURA T,NAKANISHI T,KIMURA Y,et al.Nitric oxide mediates interleukin-1-induced matrix degradation and basic fibroblast growth factor release in cultured rabbit articular chondrocytes:a possible mechanism of pathological neovascularization in arthritis[J].Endocrinology,1996,137(9):3729-3737.
[18]	[18]STADLER J,STEFANOVIC-RACIC M,BILLIAR TR,et al.Articular chondrocytes synthesize nitric oxide in response to cytokines and lipopolysaccharide[J].JImmunol,1991,Dec 1;147(11):3915-3920.
[19]	[19]SASSI N,GADGADI N,LAADHAR L,et al.Notch signaling is involved in human articular chondrocytes de-differentiation during osteoarthritis[J].J Recept Signal Transduct Res,2014,34(1):48-57.
[20]	[20]DAOUTI S,LATARIO B,NAGULAPALLI S,et al.Development of comprehensive functional genomic screens to identify novel mediators of osteoarthritis[J].Osteoarthritis Cartilage,2005,13(6):508-518.
[21]	[21]YIN S L.Effect of exercise on endothelin and nitric oxide synthesis of thoracic aorta in rat[J].Chinese Journal of Sports Medicine,1998,17(1):8-9.
[22]	[22]ZHOU Y,LIU S Q,YU L,et al.Berberine prevents nitric oxide-induced rat chondrocyte apoptosis and cartilage degeneration in a rat osteoarthritis model via AMPK and p38 MAPK signaling[J].Apoptosis,2015,20(9):1187-1199.
[23]	[23]BALAGANUR V,PATHAK NN,LINGARAJU M C,et al.Effect of S-methylisothiourea,an inducible nitric oxide synthase inhibitor,in joint pain and pathology in surgically induced model of osteoarthritis[J].Connect Tissue Res,2014,55(5-6):367-377.
[24]	[24]CUZZOCREA S.Role of nitric oxide and reactive oxygen species in arthritis[J].Curr Pharm Des,2006,12(27):3551-3570.
[25]	[25]KHOJAH H M,AHMED S,ABDEL-RAHMAN M S,et al.Reactive oxygen and nitrogen species in patients with rheumatoid arthritis as potential biomarkers for disease activity and the role of antioxidants[J].Free Radic Biol Med,2016,97:285-291.
[26]	[26]TURKON H,GOKMEN F,AKIR D U,Increased Levels of Serum Ischemia Modified Albumin in Patients with Ankylosing Spondylitis[J].Clin Lab,2016,62(4):645-649.
[27]	[27]WAHL S M,MCCARTNEY-FRANCIS N,CHAN J,et al.Nitric oxide in experimental joint inflammation.Benefit or detriment[J].Cells Tissues Organs,2003,174(1-2):26-33.
[28]	[28]ABRAMSON S B.Nitric oxide in inflammation and pain associated with osteoarthritis[J].Arthritis Res Ther,2008,10(Suppl 2):S2.
[29]	[29]ABRAMSON S B.Osteoarthritis and nitric oxide[J].Osteoarthritis Cartilage,2008,16(2):S15-20.
[30]	[30]TAKAHASHI T,KONDOH T,OHTANI M,et al.Association between arthroscopic diagnosis of temporomandibular joint osteoarthritis and synovial fluid nitric oxide levels[J].Oral Surg Oral Med Oral Pathol Oral Radiol Endod,1999,88(2):129-136.
[31]	[31]KOBAYASHI K,MATSUZAKA S,YOSHIDA Y,et al.The effects of intraarticularly injected sodium hyaluronate on levels of intact aggrecan and nitric oxide in the joint fluid of patients with knee osteoarthritis[J].Osteoarthritis Cartilage,2004,12(7):536-542.
[32]王伟,李彦林,贾福,等.骨关节炎患者软骨细胞的体外培养技术[J].昆明医学院学报,2008,29(1):149-152.

Relative Articles(20)

Relative Articles

[1]	Feifei XIE, Yinzi XIN, Min XU, Jinghan LI, Wei WANG. Mechanisms of Long Non-Coding RNAs in Cartilage Development and Related Diseases. Journal of Kunming Medical University, 2024, 45(10): 1-7. doi: 10.12259/j.issn.2095-610X.S20241001
[2]	Yihan CHEN, Shanyong ZHANG, Yu DING, Li ZHANG. Research Progress on the Molecular Pathogenesis of Temporomandibular Joint Osteoarthritis. Journal of Kunming Medical University, 2024, 45(7): 1-5. doi: 10.12259/j.issn.2095-610X.S20240701
[3]	Shaoyou DENG, Rong LI, Jintao LI, Yulan ZHAO, Peijin WANG, Hong ZHENG. Mechanism of Osteoking Improving Osteoarthritis based on Network Pharmacology and DDM Rats. Journal of Kunming Medical University, 2023, 44(7): 34-39. doi: 10.12259/j.issn.2095-610X.S20230701
[4]	Wenli HUANGFU, Yao HUANG, Bo LIU, Changhai LV, Juan LIU, Zichao DAI. Comparison of Two Induction Methods of Temporo-mandibular Joint Osteoarthritis in Rat Models. Journal of Kunming Medical University, 2023, 44(3): 49-53. doi: 10.12259/j.issn.2095-610X.S20230308
[5]	Yu LV, Yibo HU, Jiaxin WANG, Youtong SU, Youguo DAI. Advances on the Relationship between Protein S-nitrosylation and Digestive System Tumors. Journal of Kunming Medical University, 2022, 43(9): 155-159. doi: 10.12259/j.issn.2095-610X.S20220911
[6]	Zihan WANG, Gaiying YE, Tao ZHAO, Jun ZHANG, Yu HU. Experimental Study on the Expression of mTOR in TMJOA Condylar Cartilage of SD Rats. Journal of Kunming Medical University, 2022, 43(10): 10-15. doi: 10.12259/j.issn.2095-610X.S20221033
[7]	Chen Xian Wei , Lu Xiang Dong , Wang Yong Feng , Zhao Die Bo , Yang Xu , Wang Wen Xuan , Fan Yan Xin , Zhang Hui , Zhao Bin . . Journal of Kunming Medical University, 2019, 40(06): 76-79.
[8]	Yan Hui Ling , Zhao Hong Bin , Qian Chuan Yun , Wang Ying Zhong , Zhang Yang , Shen Xiang Lin , Jiang Gang , Gao Shuang . . Journal of Kunming Medical University, 2019, 40(01): 123-127.
[9]	Feng Yong Quan , Wang Jian Long , Liu Yang , Rao Lei . A Treatment Effect Study of Arthroscope Combined Proximal Humerus Osteotomy in Knee Inversion Osteoarthritis. Journal of Kunming Medical University, 2018, 39(05): 115-119.
[10]	Chen Zhong Yi , Qiao Ting Ting , Dong Bao Lian , Guo Ling . A Study of LPS Mediated Inflammatory Oxidation Reaction of Three Types of Rodents. Journal of Kunming Medical University, 2018, 39(06): 7-13.
[11]	Sun Yu Xing , Tan Li , Hao Rui , Li Ming , Gan Zheng Yan , Wu Cheng Qing . The Correlation between Exhaled Nitric Oxide and Mycoplasma Pneumoniae Pneumonia in Children. Journal of Kunming Medical University, 2017, 38(09): 79-82.
[12]	Li Xue Wu . Clinical Observation of Etofenamate Gel vs. Diclofenac Sodium in the Treatment of Knee Osteoarthritis. Journal of Kunming Medical University,
[13]	Huang Sheng Hua , Li Xiao Xia , Lian Xi Yan , Chen Zi Yao , Zhao Jing Tao , Feng Zhi Jian . Influence of Uric Acid on Human Umbillical Vein Endothelial Cells under Oxidative Stress. Journal of Kunming Medical University, 2016, 37(12): 20-23.
[14]	Dai Long Jin . . Journal of Kunming Medical University,
[15]	Zhang Xiao Qing . . Journal of Kunming Medical University,
[16]	Zhang Xiao Qing . . Journal of Kunming Medical University,
[17]	Li Fu Ping . . Journal of Kunming Medical University,
[18]	. Expression and Significance of ADAMTS-4 in Synovial Fluid of Knee Osteoarthritis. Journal of Kunming Medical University,
[19]	Song Yu Feng . Pathologic Changes of Articular Cartilage in Ovariectomied Rats. Journal of Kunming Medical University,
[20]	Wang Wei . . Journal of Kunming Medical University,