Protective Effect of Fibrauretin Injection Against Acute Lung Injury Induced by Lipopolysaccharide in Mice

Wang Ying Xia; Yang Gui Mei; Hu Yan Wen; Wang Yong Yan; Yao Shan; Zhang Xuan; Zhang Li

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Article Navigation > Journal of Kunming Medical University > 2016 > 37(12): 12-15

Wang Ying Xia , Yang Gui Mei , Hu Yan Wen , Wang Yong Yan , Yao Shan , Zhang Xuan , Zhang Li . Protective Effect of Fibrauretin Injection Against Acute Lung Injury Induced by Lipopolysaccharide in Mice[J]. Journal of Kunming Medical University, 2016, 37(12): 12-15.

Citation:

Wang Ying Xia , Yang Gui Mei , Hu Yan Wen , Wang Yong Yan , Yao Shan , Zhang Xuan , Zhang Li . Protective Effect of Fibrauretin Injection Against Acute Lung Injury Induced by Lipopolysaccharide in Mice[J]. Journal of Kunming Medical University, 2016, 37(12): 12-15.

Citation:

Wang Ying Xia , Yang Gui Mei , Hu Yan Wen , Wang Yong Yan , Yao Shan , Zhang Xuan , Zhang Li . Protective Effect of Fibrauretin Injection Against Acute Lung Injury Induced by Lipopolysaccharide in Mice[J]. Journal of Kunming Medical University, 2016, 37(12): 12-15.

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Protective Effect of Fibrauretin Injection Against Acute Lung Injury Induced by Lipopolysaccharide in Mice

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基金：国家自然科学基金资助项目(81460565); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2013FB111); 云南省中青年学术和技术带头人后备人才基金资助项目(2015HB042);

Received Date: 2016-10-10

Abstract

Abstract

Objective To investigate the protective effect of fibrauretin injection against acute lung injury induced by lipopolysaccharide(LPS) in mice.Methods Seventy-two healthy male adult Kunming mice were randomly divided into 6 goups:normal control group,LPS group(intratracheal instilation of 5 mg/kg of LPS),hydrocortisone group(intraperitoneal injection of 3.3 mg/kg of hydrocortisone,once daily for 3 days,and intratracheal instilation of 5 mg/kg of LPS on the 4th day) and low dose,medium dose and high dose of fibrauretin groups(intraperitoneal injection of 2,10 and 50 mg/kg of fibrauretin,respectively,once daily for 3 days,and intratracheal instilation of 5 mg/kg of LPS on the 4th day).The mice in each group were sacrificed by dislocation 24 h after intratracheal instilation of LPS.The lung tissues of partial mice in each group were extracted and weighed to calculate the lung coefficients,and bronchoalveolar lavage(BAL) was performed in partial mice in each group to collectthe BALF for counting the inflammatory cells.Results The lung cofficients of mice in LPS group were significantly higher than those in the normal control group(P<0.05).The lung cofficients of mice in hydrocortisone group, low dose, medium dose and high dose of fibrauretin groups were significantly lower than those in LPS group(P <0.05).For the percentage of inflammatory cells in BALF and the percentage of neutrophils in inflammatory cells,LPS group was significantly higher than the normal control group(P<0.01),and compared with LPS group,hydrocortisone group,low dose,medium dose and high dose of fibrauretin groups were significantly decreased(P<0.01).Conclusion Fibrauretin injection can significantly ameliorate the inflammatory reaction degree in mice with acute lung injury induced by LPS.
- Fibrauretin,
- Acute lung injury,
- BALF,
- Inflammatory cells,
- Lung coefficient

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References(11)

References

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