Influence of Uric Acid on Human Umbillical Vein Endothelial Cells under Oxidative Stress

Huang Sheng Hua; Li Xiao Xia; Lian Xi Yan; Chen Zi Yao; Zhao Jing Tao; Feng Zhi Jian

Volume 37 Issue 12

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Huang Sheng Hua , Li Xiao Xia , Lian Xi Yan , Chen Zi Yao , Zhao Jing Tao , Feng Zhi Jian . Influence of Uric Acid on Human Umbillical Vein Endothelial Cells under Oxidative Stress[J]. Journal of Kunming Medical University, 2016, 37(12): 20-23.

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Huang Sheng Hua , Li Xiao Xia , Lian Xi Yan , Chen Zi Yao , Zhao Jing Tao , Feng Zhi Jian . Influence of Uric Acid on Human Umbillical Vein Endothelial Cells under Oxidative Stress[J]. Journal of Kunming Medical University, 2016, 37(12): 20-23.

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Influence of Uric Acid on Human Umbillical Vein Endothelial Cells under Oxidative Stress

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基金：云南省自然科学基金资助项目(2012FB161);

Received Date: 2016-10-20

Abstract

Abstract

Objective To investigate the effects of uric acid(UA) and UA under oxidative stress on cultured human umbillical vein endothelial cells(HUVEC).Methods HUVECs were incubated with different concentration UA(0,4,8,16 mg/d L),H2O2(500 mmol/l) and UA+H2O2(500 mmol/l) for 24,48 and 72 hours.Then we observed the morphology of HUVECs and evaluated the proliferation of HUVECs by MTT assay.NO and ET-1 in supernatant medium was detected by ELISA.Results For the viability of HUVECs,there was no statistically significant difference between 4 mg/d L UA group and control group after incubation for 24,48 and 72hours(P>0.05) and between UA groups(8 mg/d L and 12 mg/d L) and control group after incubation for 24, 48 and72 hours(P>0.05).After incubation with 12 mg/d L of UA for 48 hours or 8 mg/d L of UA for 72 hours,the viability of HUVECs decreased significantly(P <0.05) The viability of HUVECs in H2O2 group decreased significantly(P<0.05).The viability of HUVECs in UA+H2O2groups after incubation for 24 h was significantly better than H2O2 group.There was no signifiant difference in the cell viability between(8 mg/d L or 12 mg/d L)UA+H2O2group and H2O2 group. Compared with the control group, the NO levels were decreased and the ET-1 levels were increased in the supernatants of HUVECs in 12 mg/d L UA group for 72 hours(P<0.05). Compared with H2O2 group,the NO levels were increased and the ET-1 levels were decreased in the supernatants of HUVECs in(8mg/d L or 12 mg/d L) UA +H2O2groups for 24 hours(P<0.05),while for(12 mg/d L) UA +H2O2group for 72 hours, the results were just the contary.Conclusion The effects of UA on HUVECs are related with both concentration and action time.Acutely increased UA may protect HUVECs form injury,while long action of UA may injure HUVECs,especially under oxidative stress.
- Uric acid,
- Hydrogen peroxide,
- Nitric oxide,
- Endothelin-1

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References

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