The Distribution and Drug-resistance of Gram-negative Bacilli among Blood Stream Infection in Yunnan Province of China during 2012～2014

Objective To learn the species distrilbution and drug-resistance of Gram-negative bacilli among bloodstream infections(BSI) in Yunnan province of China during 2012 to 2014.Methods Bloodstream infected Gram-negative bacilli were collected from 28 general hospitals in Yunan province of China.Data from all hospitals were applied with the same method of bacteria culture,iso1 ation,identification and antibiotic sensitivity tests.WHONET 5.6 was used to perform the statistical analysis.Results A total of 9042 clinical strains of non-repetitive gram-negative bacilli were collected.Enterobacteriaceae and Non-fermenter bacteria accounted for82.4% and 17.6% respectively.Enterobacteriaceae were mainly composed of Escherichia coli and Klebsiella pneumoniae.Non-fermenter bacteria were mainly composed of Acinetobacter baumannii and Pseudomonas aerouginosa.Escherichia coli accounted for the majority 49.29%, other species were Klebsiella pneumoniae13.17%, Acinetobacter baumannii 4.04% and Pseudomonas aeroginosa 3.85%.The susceptible rate of Enterobacteriaceae strains to the first generation cephalosporin was lower than 60%.The susceptible rate of E coliand Klebsiella pneumoniae to impenem was close to 100%, to Amikacin was more than 85%, to piperacillin-tazobactam was more than 70%, to Cefepime was more than 70%.But the susceptible rate of Klebsiella pneumoniae to impnem and amikacin decreased year by year from 2012 to 2014.The susceptible rate of Enterobacter cloacae to Amikacin was 86.4%~93.6%, Ciprofloxacin 70.5~76%,Cefepime 72.1~82.8%.It was less than other Enterobacteriaceae.The susceptible rate of Non-fermenter bacteria to normal antibiotics was much lower than Enterobacteriaceae.The susceptible rates of Pseudomonas aeroginosa to Impnem was 58.9%,Tobramycin 85%,Ciprofloxacin 71.7%,Amikacin 82.9%,Piperacillintazobactam 75.3%,Piperacillin59.6%,Atreonam 46.5%, Ceftazidime 69.1% and Cefepime 68.9% respectively.Furthermore, Acinetobacter baumannii's durg-resistance was more severe.The susceptible rate of Acinetobacter baumannii was lower than30%, to the third and fourth generation cephalosporin, the susceptible rate of Ampicillin/Sulbactam,Cefoperazone/Sulbactam, Carbapenems, Piperacillin/Tazuobatan, Quinolones and Carbapenems was less than40%.Conclusion Gram-negative bacilli have low susceptibilities among BSI.E.coli is the most common pathegon among BSI.The resistant rate of Non-fermenter bacteria to normal antibiotics is severe in hospitals.