Growth Difference between CNE-2 and S-18 Cell Lines after Subcutaneous Xenograft

Chen Xin; Huang Pu; Jia Nan Nan; Chen Shao Chun; Ye Pin; Liu Cheng Xing; Li Guo Ping

Volume 37 Issue 12

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Chen Xin , Huang Pu , Jia Nan Nan , Chen Shao Chun , Ye Pin , Liu Cheng Xing , Li Guo Ping . Growth Difference between CNE-2 and S-18 Cell Lines after Subcutaneous Xenograft[J]. Journal of Kunming Medical University, 2016, 37(12): 8-11.

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Chen Xin , Huang Pu , Jia Nan Nan , Chen Shao Chun , Ye Pin , Liu Cheng Xing , Li Guo Ping . Growth Difference between CNE-2 and S-18 Cell Lines after Subcutaneous Xenograft[J]. Journal of Kunming Medical University, 2016, 37(12): 8-11.

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Growth Difference between CNE-2 and S-18 Cell Lines after Subcutaneous Xenograft

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基金：国家自然科学基金资助项目(81260195;81302362);

Received Date: 2016-09-22

Abstract

Abstract

Objective To study the growth difference and possible mechanism between nasopharyngeal carcinoma(NPC) cell line CNE-2 and its subclone S-18.Methods CNE-2 and S-18 cells were cultured in vitro.6×105 cells/mouse were xenografted subcutaneously in the back of nude mice.The volumes of tumors were measured on the 3 rd,7 th,10 th,14 th day after grafting.Mice were sacrificed on the 14 th day and tumors were isolated and weighed.RNA from tumor tissues were extracted and transcriptional levels of HSP27 and NF-ΚB were detected.Results(1) S-18, instead of CNE-2, grew to form tumor mass 7 days after xenografting subcutaneously; both cell lines formed tumor mass 10 days after xenografting, however, the volumes of S-18 tumors [(223.13±21.32) mm3, 10 th day;(420.25±24.52) mm3,14 th day] were significant bigger than CNE-2 tumors [(113.70±11.70) mm3,10 th day;(279.86±25.78) mm3,14 th day];The weights of S-18 tumors were significantly higher than CNE-2 tumors on the 14 th day after xenografting;(2) The transcriptional levels of HSP27 and NF-ΚB in S-18 tumor were significantly higher than in CNE-2 tumor.Conclusion Xenografted S-18 NPC grows faster than Xenografted CNE-2 NPC.HSP27 and NF-κB are probably involved in the regulation of growth in NPC.
- CNE-2 cell line,
- S-18 cell line,
- HSP27,
- NF-κB,
- Tumor growth

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