IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway

Lei Wen

Article Contents

Article Navigation > Journal of Kunming Medical University > 2016 > 37(05):

Lei Wen . IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway[J]. Journal of Kunming Medical University, 2016, 37(05).

Citation:

Lei Wen . IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway[J]. Journal of Kunming Medical University, 2016, 37(05).

Lei Wen . IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway[J]. Journal of Kunming Medical University, 2016, 37(05).

Citation:

Lei Wen . IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway[J]. Journal of Kunming Medical University, 2016, 37(05).

PDF( 467 KB)

IL-27 Alleviates the Bleomycin-induced Pulmonary Fibrosis through Regulating TGF-β/Smad Signaling Pathway

Lei Wen

(Dept. of Respiratory Medicine,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China)

Abstract

Abstract

［Abstract］Objective To explore whether IL-27 inhibited the pulmonary fibrosis through regulating the expression of TGF-β/Smad signaling pathway in the bleomycin-induced pulmonary fibrosis model. Methods Forty male C57/BL6 mice were randomly divided into normal control group（group A）， bleomycin-induced pulmonary fibrosis group （group B）， bleomycin+IL-27 group（group C）and bleomycin+IL-27 antibody group （group D） with 10 in each. Five mice in each group were sacrificed on days 7 and 28 after with intratracheal bleomycin. TGF-βR1， Smad1 and Smad3 in right lung tissue were measured by Western Blot. Results 1. In the bleomycin-induced pulmonary fibrosis model， the expression of TGF-βR1 was higher on days 7 and 28， which was inhibited by IL-27. 2. The expressions of p-Smad1 and p-Smad3 were highest in group D on days 7 and 28， but were lower in group C on day 7 than those in group B. Conclusion Exogenous IL-27 might alleviate pulmonary fibrosis through inhibiting the related protein phosphorylation in TGF-β/Smad signaling pathway.
- ［Key words］IL-27；Pulmonary fibrosisp；TGF-β/Smad signaling pathway,

FullText(HTML)

References(0)

References

Relative Articles(20)

Relative Articles

[1]	Yi HUANG, Yongjian HUANG, Ling MAI, Ting LUO. Relationship between TGF-β/Smads Signaling Pathway and Cognitive Dysfunction in Patients with Aneurysmal Subarachnoid Hemorrhage. Journal of Kunming Medical University, 2024, 45(3): 127-132. doi: 10.12259/j.issn.2095-610X.S20240319
[2]	Cong LIU, Guishuai WU, Rui PU, Shude LI, Jianping TAO, Renfa ZHANG. EGCG Improves Myocardial Fibrosis Induced by High Fat and High Sugar Diet in Obese Rats by Inhibiting TGF-β1/Smads Signaling Pathway. Journal of Kunming Medical University, 2022, 43(5): 18-26. doi: 10.12259/j.issn.2095-610X.S20220506
[3]	Yegang HU, Wei ZHANG. Role of Hereditary T Lymphocyte Immune Deficiency in Pulmonary Fibrosis Induced by Paraquat. Journal of Kunming Medical University, 2022, 43(4): 6-11. doi: 10.12259/j.issn.2095-610X.S20220402
[4]	Ai-ping XU, Hua LIN, Li-hui GAO, Ling LI, Meng-wei CHENG, Ge WANG, Juan YANG, Yan-fen NIU. Effect of Norathyriol on the Epithelial-mesenchymal Transition of HK-2 Cells Induced by TGF-β1. Journal of Kunming Medical University, 2021, 42(7): 1-6. doi: 10.12259/j.issn.2095-610X.S20210701
[5]	Na PI, Qin HE, Yan ZHONG, Xuan JIN, Zhen-dong ZHU, Xuan ZHANG. Effect of Gentiana Rigescens Franch on Expression of NF - κ B and CTGF in Lung Tissue of Mice with Pulmonary Fibrosis. Journal of Kunming Medical University, 2021, 42(4): 8-12. doi: 10.12259/j.issn.2095-610X.S20210402
[6]	Yong-jun LIU, Yin WANG, Mei YANG, E-gao YIN, Ting LI, Zhao-xing DONG. The Effect of Hydrogen-Regulated Autophagy on Pulmonary Fibrosis in Paraquat Poisoned Rats. Journal of Kunming Medical University, 2021, 42(9): 1-6. doi: 10.12259/j.issn.2095-610X.S20210918
[7]	Wang Jun, Zhang Yi , Zhang Shi Hui , Jin Fu Yu , Li Tian , Xu Hong . . Journal of Kunming Medical University, 2020, 41(04): 6-10.
[8]	Li Ting , Deng Shu Hao , Liu Yong Jun , Dong Zhao Xing . . Journal of Kunming Medical University, 2019, 40(08): 1-5.
[9]	Zheng Hong , Li Jin Tao , Yuan Xin , Wu Chao , Zeng He , Zhao Hong Bin , Tang Wei . Osteoking Promotes the Proliferation of BMSC through Activating TGF-β/Smad Signaling Pathway in Castrate Osteoporosis Rats. Journal of Kunming Medical University, 2018, 39(03): 5-10.
[10]	Tai Wen Lin , Ding Jia Wei , Zheng Bo Yang , Wu Han Xin , Gao Ling , Zhang Wei , Dong Zhao Xing , Xu Yi Heng . . Journal of Kunming Medical University, 2017, 38(03): 21-26.
[11]	Long Rui Qing , Liu Zhuo Hui , Zhang Fan , Li Shu Ling , Yu Yong Mei , Ruan Biao . Infection of Pseudomonas aeruginosa Activates TGF-β1 Smad Signaling Pathway in Human Middle Ear Epithelial Cells. Journal of Kunming Medical University, 2017, 38(10): 65-69.
[12]	Cao Yong Mei , Lv Yi , Liu Yu Jing , Li Ying Chuan . The Effect of miR-200 and Their Targets ZEB1/2 on Epithelial-mesenchymal Transition(EMT) in Early Pulmonary Fibrosis Caused by Acute Lung Injury. Journal of Kunming Medical University, 2016, 37(12): 1-7.
[13]	Wang Ying Xia . Anti-fibrotic Effect of Kudancao Tablets on Bleomycin-induced Pulmonary Fibrosis in Rats. Journal of Kunming Medical University,
[14]	Dong Zhao Xing . . Journal of Kunming Medical University,
[15]	He Xiao Yu . . Journal of Kunming Medical University,
[16]	Zhang Ping . . Journal of Kunming Medical University,
[17]	Yang Jing . . Journal of Kunming Medical University,
[18]	Shi Hai Ying . . Journal of Kunming Medical University,
[19]	. Expression of CNTF, TGF-β1, and IGF-1 in NSCs Cultured in vitro. Journal of Kunming Medical University,
[20]	Zhang Ji Zhou . Effect of Exogenous Carbon Monoxide on Gaseous Signaling Molecules in Hippocampus of Rats with Cerebral Ischemic-Reperfusion Injury. Journal of Kunming Medical University,