Application of 13 High-risk HPV infection Test Combined with Thinprep Cytologic Test on Screening Cervical Carcinoma in Dali Region， Yunnan Province

［Abstract］Objective　To investigate relativity between the epidemiology of HPV and cervical carcinoma in Dali region，Yunnan province，through detecting the 13 high-risk human papillomavirus infection and Thinprep cytologic test in 2153 cases. Methods Real-time PCR was used to detect the 13 high-risk HPV（16，18，31，33，35，39，45，51，52，56，58，59，68）in 2153 cases and 1604 cases were checked with Thinprep cytologic test. Results In 2153 samples， 260 cases were infected with HPV， with the positive rate of 12.08％. The highest positive rates were ＞60 years old（18.18%）， then ＞20 and ≤30 years old （14.41%）; there was no significant difference in the positive rate among the various age groups （P =0.384）．There were 1465 negative for intraepithelial lesion ormalignancy（NILM）cases（91.33%）， 86 atypical squamous cells of undetermined significance（ASC-US）and atypical squamous cells cannot exclude HSIL（ASC-H） cases（5.36%）， 32 low-grade squamous intraepithelial lesion cases（LSIL） cases（2.00%）， 21 high-grade squamous intraepithelial lesion cases（1.31%）through Thinprep cytologic test. The correlation coefficient is 0.893.Conclusions The infection rate of HPV in Dali region， Yunnan Province， has no significant difference among the various age groups．Application of 13 high-risk HPV infection test combined with Thinprep cytologic test could be more effective in screening cervical carcinoma．