Impact on the Expression of CD123 on K562 Cells Induced by rhIFN-γ

［Abstract］Objective　To investigate the inhibitory effect of rhIFN-γ on human chronic myeloid leukemia cell line K562，and the impaction on the expression of CD123．Methods　MTT method was used to test cell relative viability with rhIFN-γ at different concentrations. The expression of CD123 on K562 cells was detected by flow cytometry. Results　The relative inhibition of K562 cells proliferation was hampered when the cells were treated with rhIFN-γ for 72 h at the concentration of 5×104～108 U/L，respectively. However， rhIFN-γ at 2×105 U/L was benefit to K562 cells proliferation. After treatment with rhIFN-γ at 0， 2×105 U/L and 107 U/L， the percentages of CD123 expression on K562 cells were （4.10±1.46）%，（7.2±2.50）% as well as （21.6±1.17）%，respectively. Compared with the control group， 107 U/L rhIFN-γ significantly increased the expression of CD123 on K562 cells （P＜0.05）. Conclusion　The effect of rhIFN-γ on the growth of K562 cells has two aspects （inhibition or proliferation），and it can increase the expression of CD123.