Li Jun Jie . The Gene Expression Change of Inflammatory Factors TNF-α, IL-6 and IL-1βin Cerebral Ischemia-Reperfusion Rats[J]. Journal of Kunming Medical University, 2016, 37(09).
Citation: Li Jun Jie . The Gene Expression Change of Inflammatory Factors TNF-α, IL-6 and IL-1βin Cerebral Ischemia-Reperfusion Rats[J]. Journal of Kunming Medical University, 2016, 37(09).

The Gene Expression Change of Inflammatory Factors TNF-α, IL-6 and IL-1βin Cerebral Ischemia-Reperfusion Rats

  • [Abstract]Objective To investigate the gene expression change of TNF-α, IL-6 and IL-1β at different time points in brain tissues of rats with cerebral ischemia reperfusion injury. Methods A total of 24 adult male SD rats were randomly divided into four groups: sham group and 3 groups with brain ischemia reperfusion of 3h, 6h and 12h. Real-Time PCR was used to analyze the gene expression of TNF-α, IL-6 and IL-1β at 3h, 6h, and 12h after reperfusion. Results In the sham group, the mRNA expression levels of TNF-α, IL-6 and IL-1βin were low, but increased immediately after brain ischemia injury and decreased gradually thereafter. The gene expression of TNF-α mRNA at 3h after reperfusion was significantly increased and reached the peak (P<0.01) then significantly decreased at 12h after reperfusion. The gene expression of IL-6 mRNA was notably increased at 3h after reperfusion and peaked at 6h (P<0.01), and significantly decreased at 12h compared with 6h (P<0.01). The gene expression of IL-1βmRNA at 3h after reperfusion was significantly increased, peaked at 6h (P<0.01) and significantly decreased at 12h (P<0.01). Conclusion The gene expression levels of TNF-α, IL-6 and IL-1β mRNA increased significantly in the early stage of reperfusion and decreased gradually after reaching the peak, which suggested that the gene expression change of TNF-α,IL-6 and IL-1β was involved in the mechanism of cerebral ischemia reperfusion injury.
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