Cuture of Regulatory T Cells and the Changes of Immune Factors in COPD Rats after Venous injection of Regulatory T Cells

［Abstract］Objective　To study the change of immune factor in COPD rats after intravenous injection of regulatory T cells. Methods Twenty-one SPF rats was divided into three groups at random， rat COPD model was built by smoking. We used magnetic bead isolation technic to separate CD4+CD25+ regulatory T cells. Regulatory T cells were cultured and injected into rats though rats' caudal vein according to different dose， 5×104/mL，5×105/mL，5×106/mL respectively. Flow cytometry was used to analyze cell factors. ELISA was used to analyze IL-6 and CRP．Results　Adding JJ316 or IL-2 into medium benefited the proliferation of CD4+CD25+ regulatory T cells. On the 20 th day， regulatory T cells CD4+CD25+ proliferation stopped by adding JJ316 or IL-2 respectively. Regulatory T cells were cultured and injected into rats though rat caudal vein according to different dose. The levels of CRP and IL-6 were decreased when rats were injected by CD4+CD25+regulatory T cell after one week. Conclusions　Injection of regulatory T cells is helpful to control inflammation progression of COPD， so the increase of regulatory T cells of patients with COPD may decrease inflammation progression of COPD.