Experimental Research on Liver Pathological Characteristics of Autoimmune Hepatitis C57BL/6 Mice

［Abstract］Objective To observe liver pathological characteristics of the autoimmune hepatitis （AIH） C57BL/6 mice．Methods　We established AIH C57BL/6 mice model with mixed adjuvants of allogenic liver antigens and complete freund's adjuvant by intraperitoneal injection. Then we determined and compared the body weight， alanine aminotransferase（ALT），aspartate aminotransferase（AST） between the normal control group （N group） and AIH model group（M group）. And we compared the pathological characteristics of liver，spleen， heart， lung， kidney between two groups. Results　We successfully established AIH C57BL/6 mice model. Compared with N group， we found that the average weight increase of mice in M group was decreased，ALT and AST of mice in M group were boosted. In M group， liver of mice presented typical interface of hepatitis， lymphocytic infiltration，even severe hepatitis，and showed spotty necrosis， multi-acinar necrosis. Some showed early sign of liver fibrosis by aprearing fibrous tissue hyperplasia. M group mouse's spleens were enlarged significantly. The spleen had darker color， not neat， and not smooth. Meanwhile， results were analyzed with statistics to confirm whether there was a significant difference between two groups（P＜0.05）. About lungs， hearts and kidneys of mice in two groups，the pathological features were not found， and there was no statistic difference （P＞0.05）. Conclusions　The liver pathological characteristics of AIH C57BL/6 mice are similar to AIH patients. The results provide the pathological basis for the experimental research of autoimmune hepatitis.