Clinical Significance of Examination of Pretreatment Peripheral Blood HPV16E6 mRNA and CK19 mRNA in Cervical Cancer Patients

Yang Lin Lin; Wang Wei; Zhang Lei; Zhang Hong Ping; Yang Hong Ying

Article Contents

Article Navigation > Journal of Kunming Medical University > 2017 > 38(01): 23-26

Yang Lin Lin , Wang Wei , Zhang Lei , Zhang Hong Ping , Yang Hong Ying . Clinical Significance of Examination of Pretreatment Peripheral Blood HPV16E6 mRNA and CK19 mRNA in Cervical Cancer Patients[J]. Journal of Kunming Medical University, 2017, 38(01): 23-26.

Citation:

Yang Lin Lin , Wang Wei , Zhang Lei , Zhang Hong Ping , Yang Hong Ying . Clinical Significance of Examination of Pretreatment Peripheral Blood HPV16E6 mRNA and CK19 mRNA in Cervical Cancer Patients[J]. Journal of Kunming Medical University, 2017, 38(01): 23-26.

Citation:

Yang Lin Lin , Wang Wei , Zhang Lei , Zhang Hong Ping , Yang Hong Ying . Clinical Significance of Examination of Pretreatment Peripheral Blood HPV16E6 mRNA and CK19 mRNA in Cervical Cancer Patients[J]. Journal of Kunming Medical University, 2017, 38(01): 23-26.

PDF( 166 KB)

Clinical Significance of Examination of Pretreatment Peripheral Blood HPV16E6 mRNA and CK19 mRNA in Cervical Cancer Patients

Funds:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB168);

Received Date: 2016-09-20

Abstract

Abstract

Objective To investigate the significance of pretreatment peripheral blood HPV16E6m RNA and CK19m RNA in diagnosis and their correlations to the clinicopathologic features of cervical carcinoma.Methods One hundred cervical carcinoma patients underwent pretreatment peripheral blood HPV16E6m RNA and CK19m RNA evaluation;40 healthy women were subjected as control.The specificity and sensitivity of HPV16E6m RNA and CK19m RNA as diagnostic indexes were analyzed; their correlations to clinicopathologic features were investigated.Results The specificity of HPV16E6m RNA and CK19m RNA in the diagnosis of cervical cancer were 97.5%.The sensitivity of HPV16E6m RNA and CK19m RNA in the diagnosis of pre treatment were 15% and39%, respectively and the difference was statistically significant (P <0.05) .HPV16E6m RNA elevation was associated with clinical stage (P= 0.012) and pelvic lymph node metastasis (P=0.026) . CK19m RNA was associated with deep muscular invasion (P=0.031) and pelvic lymph node metastasis (P=0.027) .Conclusion Peripheral blood HPV16E6m RNA and CK19m RNA are the valuable tumor markers of cervical cancer.The value of CK19m RNA in the diagnosis of cervical cancer is better than that of HPV16E6m RNA.
- Cervical neoplasm,
- HPV16E6m RNA,
- CK19m RNA,
- Peripheral blood

FullText(HTML)

References(15)

References

[1]	[1]QMICHOU Z, KHYATTI M, BERRAHO M, et al.Analysis of mutations in the E6 oncogene of human papillomavirus 16 in cervical cancer isolates from Moroccan women[J].BMC Infect Dis, 2013, 13 (8) :378.
[2]	[2]SHI Y l, LI L, HU Z, et al.A genome-wide association study identifies two new cervical cancer susceptibility loci at4q12 and 17q12[J].Nat Genet, 2013, 45 (8) :918-922.
[3]	[3]JIANG B, XUE M.Correlation of E6 and E7 levels in high-risk HPV16 type cervical lesions with CCL20 and Langerhans cells[J].Genet Mol Res, 2015, 14 (3) :10473-10481.
[4]	[4]LEE S J, LEE A W, KANG C S, et al.Clinicopathological implications of human papilloma virus (HPV) L1 capsid protein immunoreactivity in HPV16-Positive cervical cytology[J].Int J Med Sci, 2014, 11 (1) :80-86.
[5]	[5]TORTORA M, ANNUNZIATA C, LIGUORI G, et al.Detection of human papillomavirus DNA in peri-tumor tissues and pelvic lymph nodes as potential molecular marker of micrometastasis in cervical cancer[J].Infect Agent Cancer, 2016, 11 (1) :1-7.
[6]	[6]ZHAO S, YANG H, ZHANG M, et al.Circulating tumor cells (CTCs) detected by triple-marker Ep CAM, CK19, and h MAM RT-PCR and their relation to clinical outcome in metastatic breast cancer patients[J].Cell Biochem Biophys, 2013, 65 (2) :263-273.
[7]	[7]SUN D W, ZHANG Y Y, SUN X D, et al.Prognostic value of cytokeratin 19 in hepatocellular carcinoma:A meta-analysis[J].Clin Chim Acta, 2015, 25 (8) :161-169.
[8]	[8]ZHANG X, CHEN S B, CHEN J X, et al.CK19 m RNA expression in the bone marrow of patients with esophageal squamous cell carcinoma and its clinical significance[J].Dis Esophagus, 2010, 23 (5) :437-443.
[9]	[9]YOSHIDA K, SATO K, TONOGI M, et al.Expression of Cytokeratin 14 and 19 in Process of Oral Carcinogenesis[J].Bull Tokyo Dent Coll, 2015, 56 (2) :105-111.
[10]	[10]LIU Z, YU P, XIONG Y, et al.Significance of CK19, TPO, and HBME-1 expression for diagnosis of papillary thyroid carcinoma[J].Int J Clin Exp Med, 2015, 8 (3) :4369-4374.
[11]	[11]ZHOU L, ZHANG H, HUANG C, et al.Expression of Daxx and HPV16 E6 and its significance in cervical lesions[J].Zhonghua Yi Xue Za Zhi, 2015, 95 (37) :3050-3053.
[12]	[12]YANG X, LU L.Expression of HPV-16 E6 protein and p53 inactivation increases the uterine cervical cancer invasion[J].Drug Res, 2015, 65 (2) :70-73.
[13]	[13]OKAMOTO S, NIIKURA H, NAKABAYASHI K, et al.Detection of sentinel lymph node metastases in?cervical cancer:assessment of KRT19 m RNA in the one-step nucleic acid amplification (OSNA) method[J].Gynecol Oncol, 2013, 130 (3) :530-536.
[14]	[14]ZHANG F, LIU D, LIN B, et al.Expression of high-risk HPV DNA and CK19 in pelvic lymph nodes in stage Ia-IIa cervical cancer and their clinical value[J].Oncol Rep, 2012, 27 (6) :1801-1806.
[15]	[15]REN C, HAN C, FU D, et al.Circulating tumor cells in breast cancer beyond the genotype of primary tumor for tailored therapy[J].Int J Cance, 2016, 138 (7) :1586-1600.

Relative Articles(20)

Relative Articles

[1]	Xiangjie CUI, Yufen TAO, Lanfang ZHU, Yufeng YAO, Li SHI. Evaluations of Immunogenicity and Efficacy of A Novel HPV16 E6 and E7 Multi-epitope DNA Vaccine. Journal of Kunming Medical University, 2024, 45(5): 16-22. doi: 10.12259/j.issn.2095-610X.S20240503
[2]	Xiangchuan QIN, Jinqiu LI, Xiaojing HUANG, Kuerban HUTUBIDING·, Hasim AXIANGU·. Effect of HPV E6 on Proliferation，Invasion and Migration of Cervical Cancer Cells Through Rap1 Signaling Pathway. Journal of Kunming Medical University, 2024, 45(9): 9-16. doi: 10.12259/j.issn.2095-610X.S20240902
[3]	Jinbao XIAO, Junda ZHAO, Junqi MA. Metabolomics of Siha Supernatant in Cervical Cancer Cells with Down-regulated HPV16 E6/E7 Expression. Journal of Kunming Medical University, 2024, 45(1): 22-27. doi: 10.12259/j.issn.2095-610X.S20240104
[4]	Jingyu YAO, Rui WEI, Lin ZHANG, Shengliu SHI, Peng BAI. The Effects of NLR，PLR，and FRA in Peripheral Blood on Normal Bone Mass and Bone Loss after Fractures. Journal of Kunming Medical University, 2024, 45(11): 123-127. doi: 10.12259/j.issn.2095-610X.S20241117
[5]	Xiaoxiao ZHANG, Lulu LIU, Feng YAN, Jinlan YANG, Zhiqiang WANG, Meili HU. Analysis of Results from AI-Assisted Cervical Cancer Screening for Rural Women in Baoding City. Journal of Kunming Medical University, 2024, 45(10): 135-139. doi: 10.12259/j.issn.2095-610X.S20241021
[6]	Fangyuan GUO, Wenliang LI, Fan XU, Xiangqun WEI. The Expression and Clinical Significance of Non-coding RNA in Cervical Cancer. Journal of Kunming Medical University, 2022, 43(5): 108-116. doi: 10.12259/j.issn.2095-610X.S20220527
[7]	Honglan CHEN, Yi WU, Tao HU, Yuming WANG. Potential Value of G6PD Activity Detection in the Peripheral Blood for the Diagnosis and Prognosis of Cervical Cancer Patients with High Risk Human Papillomavirus Infection. Journal of Kunming Medical University, 2022, 43(2): 89-95. doi: 10.12259/j.issn.2095-610X.S20220203
[8]	Lin-lin YANG, Chun-mei YIN, Lei ZHANG, Wei WANG, Ling-feng ZHAO, Yu-die YANG, He ZHAO, Ying LIU. Expression of HPV16E6 and Indoleamine 2, 3-dioxygenase in Cervical Lesions and Its Clinical Implications. Journal of Kunming Medical University, 2020, 41(12): 68-72. doi: 10.12259/j.issn.2095-610X.S20201219
[9]	Li Hui , Ping Qin Rong , Shi Yun Qiang , Wang Chun Hui , Yang Yang , Hu Li Bing , Bi Xiao Fang , Li Jian , Zhong Yi Ming . . Journal of Kunming Medical University, 2020, 41(01): 52-56.
[10]	Sun Chun Yi , Xu Chang Jun , Huang Ya Juan , Qing Qing , Li Dai Zhu , Liu Yang . . Journal of Kunming Medical University, 2019, 40(02): 49-53.
[11]	Yan Yong , Sun Xuan , Li Gang , Zheng Cuan , Ma Shao Xiang , Li Yong , Zhao Run Heng , Wang Ke . Expression of Survivin in Circulating Tumor Cells in Patients with Bladder Cancer. Journal of Kunming Medical University, 2017, 38(07): 102-106.
[12]	Li Peng . The Difference in the Concentration of Insulin and c-peptide in Portal Vein and Peripheral Blood. Journal of Kunming Medical University,
[13]	Wang Yang . . Journal of Kunming Medical University,
[14]	Chen Xiao Qun . . Journal of Kunming Medical University,
[15]	Li Wen Liang . . Journal of Kunming Medical University,
[16]	Wei Xiang Qun . . Journal of Kunming Medical University,
[17]	Wei Xiang Qun . . Journal of Kunming Medical University,
[18]	Zhou Shi Qiao . . Journal of Kunming Medical University,
[19]	. Determination of Concentration of 5-Fluorouraci in Blood of Cancer Patients by HPLC. Journal of Kunming Medical University,
[20]	. Construction and Identification of Short Hairpin RNA Lentiviral Vector of Human Papillomavirus (HPV) 16 E6. Journal of Kunming Medical University,