The Application of Loop-mediated Amplification Chip in Gene Testing of Common Lower Respiratory Pathogens

Tang Rui Zhu; Luo Zheng Qiong; Xu Qiu Yue; Zhao Ying; Li Huan; Wang Yun Juan; Song Yu; Liu Zi Jie

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Tang Rui Zhu , Luo Zheng Qiong , Xu Qiu Yue , Zhao Ying , Li Huan , Wang Yun Juan , Song Yu , Liu Zi Jie . The Application of Loop-mediated Amplification Chip in Gene Testing of Common Lower Respiratory Pathogens[J]. Journal of Kunming Medical University, 2017, 38(01): 8-12.

Citation:

Tang Rui Zhu , Luo Zheng Qiong , Xu Qiu Yue , Zhao Ying , Li Huan , Wang Yun Juan , Song Yu , Liu Zi Jie . The Application of Loop-mediated Amplification Chip in Gene Testing of Common Lower Respiratory Pathogens[J]. Journal of Kunming Medical University, 2017, 38(01): 8-12.

Citation:

Tang Rui Zhu , Luo Zheng Qiong , Xu Qiu Yue , Zhao Ying , Li Huan , Wang Yun Juan , Song Yu , Liu Zi Jie . The Application of Loop-mediated Amplification Chip in Gene Testing of Common Lower Respiratory Pathogens[J]. Journal of Kunming Medical University, 2017, 38(01): 8-12.

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The Application of Loop-mediated Amplification Chip in Gene Testing of Common Lower Respiratory Pathogens

Tang Rui Zhu ^1,2,3,
Luo Zheng Qiong ⁴,
Xu Qiu Yue ^1,2,3,
Zhao Ying ^1,2,3,
Li Huan ^1,2,3,
Wang Yun Juan ^1,2,3,
Song Yu ^1,2,3,
Liu Zi Jie ^1,2,3

Funds:

基金：国家自然科学基金资助项目 (81460357);

Received Date: 2016-11-10

Abstract

Abstract

Objective To investigate the value of Loop-mediated Amplification (LAMP) in the gene diagnosis of common pathogens in lower respiratory infection.Methods One hundred and twenty-two cases of lower respiratory tract secretions and bronchoalveolar lavage fluid were collected from patients with suspected lower respiratory infection.The pathogens were tested by using LAMP, isolation and cultivation of microbial pathogens, real-time FQ-PCR.The results were compared with different specimen types, age and detection methods.Statistical analysis was performed using the SPSS software and Kappa test.Results In 122 cases of specimensfrom patients with respiratory infection, the positive rate of sputum was higher (55.56%) than that in bronchoalveolar lavage fluid (46.88%) .The main pathogens were Methicillin-resistant Staphylococcus (MASA) and mycoplasma pneumoniae (MP) (P<0.05) respectively.In different age stage, MASA was mainly detected in the elder group (>60 years) , and MP was the main pathogen in the younger patients (≤20 years) (P<0.01) .The detection rate of LAMP (50.00%) was higher than the microbial Isolation and cultivation (36.67%) .The detection rates of Klebsiella pneumoniae (Kpn) and Bauman Acinetobacter (CSAB) by two different methods were consistent (kappa>0.4) , however the consistencies in other pathogens were low (kappa<0.4) .And there was a high consistency between LAMP and real-time FQ-PCR in the detection of MP (kappa >0.7) .Conclusions LAMP has the advantages of rapidity, sensitivity, high effciency and specificity, high throughput and wide coverage of pathogens, which may be a quick and reliable laboratory diagnosis method.And using different respiratory specimens or methods may have different results.They have their own advantages and disadvantages, which are needed to be paid close attention by clinicians.
- Lower respiratory infection,
- Pathogen,
- Loop-mediated amplification

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References(10)

References

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