The Changes and Sources of Cytokines in the Retina of Rats after Traumatic Optic Nerve Injury

Nie Zu Qing; Zhang Qiong; Wang Peng Fei; Yang Li Juan; Yang Wen Hong; Wang Ruo Hua; Ma Lin Kun; Yang Ming

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Article Navigation > Journal of Kunming Medical University > 2017 > 38(04): 22-27

Nie Zu Qing , Zhang Qiong , Wang Peng Fei , Yang Li Juan , Yang Wen Hong , Wang Ruo Hua , Ma Lin Kun , Yang Ming . The Changes and Sources of Cytokines in the Retina of Rats after Traumatic Optic Nerve Injury[J]. Journal of Kunming Medical University, 2017, 38(04): 22-27.

Citation:

Nie Zu Qing , Zhang Qiong , Wang Peng Fei , Yang Li Juan , Yang Wen Hong , Wang Ruo Hua , Ma Lin Kun , Yang Ming . The Changes and Sources of Cytokines in the Retina of Rats after Traumatic Optic Nerve Injury[J]. Journal of Kunming Medical University, 2017, 38(04): 22-27.

Citation:

Nie Zu Qing , Zhang Qiong , Wang Peng Fei , Yang Li Juan , Yang Wen Hong , Wang Ruo Hua , Ma Lin Kun , Yang Ming . The Changes and Sources of Cytokines in the Retina of Rats after Traumatic Optic Nerve Injury[J]. Journal of Kunming Medical University, 2017, 38(04): 22-27.

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The Changes and Sources of Cytokines in the Retina of Rats after Traumatic Optic Nerve Injury

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基金：云南省中青年学术技术带头人后备人才基金资助项目(2015HB041); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2015FA006,2015FB055);

Received Date: 2017-02-21

Abstract

Abstract

Objective To explore the immunoregulatory mechanisms of glial cells in retina after optic nerve injury in rats by using rats with traumatic optic nerve injury as TON model to detect the activation of microglia,astrocyte,and observe the temporal and spatial expression of inflammatory mediators and neurotrophic factors in retina at different time points after TON. Methods We established an animal model of rat optic nerve traumatic and draw materials from the rats at 1d, 3d, 5d and 7d after injury.Western-blot was used to dectect the expression levels of IL-1β, TNF-α and iNOS at all time points after injury. Immunofluorescence staining was used to detect microglia, astrocytes and the specific lesions expression and distribution of IL-1β,TNF-α,iNOS,Nestin inthe retina. Results Compared with the control group, the expression of IL-1β protein was peaked at 3 days after injury, and the difference was statistically significant(P <0.05), the expression declined at 5 days, the difference was not statistically significant(P>0.05). The expression of TNF-α increased at all time points after injury(P<0.05), and the expression level of TNF-α reached the peak at 3 days after injury. Compared with the control group, the difference was a statistical significant(P <0.01). The level of iNOS was significantly up-regulated at 1 day after injury, and the expression level of iNOS was significantly higher than the control group,and the difference was statistically significant(P<0.05), the expression declined at 3 days, the difference was not statistically significant(P>0.05). Immunohistochemical staining showed that after the injury,we can observe the activation of microglia and astrocytes. IL-1β and iNOS were expressed in activated microglia and astrocytes.But we were unable to observe the expression of TNF-α in astrocytes at all time points. Nestin expression was observed in astrocytes at 7 days after injury. Conclusions The activated astrocytes and microglia in retina are the main sources of IL-1β and iNOS after traumatic optic nerve injury. The activated astrocytes may have neuroprotective effects in the model.
- Optic nerve injury,
- Retina,
- Microglia,
- Astrocyte,
- Activation,
- IL-1β,
- TNF-α,
- iNOS,
- Nestin

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References(16)

References

[1]	[1]ANDERSON R L,PANJE W R,GROSS C E.Optic nerve blindness following blunt forehead trauma[J].Ophthalmology,1982,89(5):445.
[2]	[2]LEVKOVITCHVERBIN H,HARRISCERRUTI C,GRONER Y,et al.RGC death in mice after optic nerve crush injury:oxidative stress and neuroprotection.[J].Investigative Ophthalmology&Visual Science,2001,41(13):4169-4174.
[3]	[3]MIRON V E,BOYD A,ZHAO J W,et al.M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination[J].Nature Neuroscience,2013,16(9):1211-1218.
[4]	[4]HANISCH U K,KETTENMANN H.Microglia:Active sensor and versatile effector cells in the normal and pathologic brain.[J].Nature Neuroscience,2007,10(11):1387.
[5]LI HONG SHEN,QI GAO,YI LI,et al.Down-regulation of neurocan expression in reactive astrocytes promotes axonal regeneration and facilitates the neurorestorative effects of bone marrow stromal cells in the ischemic rat brain[J].神经损伤与功能重建,2008,3(6):404-410.
[6]	[6]YIN Y,CUI Q,LI Y,et al.Macrophage-derived factors stimulate optic nerve regeneration.[J].Journal of Neuroscience the Official Journal of the Society for Neuroscience,2003,23(6):2284-2293.
[7]	[7]LIH Y,RUANYW,RENC R,et al.Mechanisms of secondary degeneration after partial optic nerve transection[J].Neural regeneration research,2014,9(6):565-565.
[8]李艳,孙丽,韩宏艳,等.NO及其合酶在大鼠视神经夹持模型上的表达变化及其作用.昆明医科大学学报[J].2015,36(7):33-39.
[9]	[9]TEZEL G.TNF-αsignaling in glaucomatous neurodegeneration[J].Progress in Brain Research,2008,173(1):409-421.
[10]	[10]TEZEL G,WAX M B.The immune system and glaucoma[J].Current Opinion in Ophthalmology,2004,15(15):80-84.
[11]	[11]HU X,LEAK R K,SHI Y,et al.Microglial and macrophage polarization-new prospects for brain repair[J].Nature Reviews Neurology,2015,11(1):56-64.
[12]	[13]DAVID S,KRONER A.Repertoire of microglial and macrophage responses after spinal cordinjury[J].Nature Reviews Neuroscience,2011,12(7):388.
[13]	[12]KITAOKA Y,KITAOKA Y,KWONG J M K,et al.TNF-α-induced optic nerve degeneration and nuclear factor-κB p65[J].Investigative Ophthalmology&Visual Science,2006,47(4):1448-1457.
[14]刘鹏种,陆世铎,黄娅林,等.成年鼠缺血性脑损伤诱导nesitn的表达[J].生理学报,2002,54(4):294-299.
[15]	[15]FANG M,YUAN Y,RANGARAJAN P,et al.Scutellarin regulates microglia-mediated TNC1 astrocytic reaction and astrogliosis in cerebral ischemia in the adult rats[J].BMC Neuroscience,2015,16(1):84.
[16]	[16] YUAN Y,ZHA H,RANGARAJAN P,et al.Anti-inflammatory effects of Edaravone and Scutellarin in activated microglia in experimentally induced ischemia injury in rats and in BV-2 microglia[J].BMC Neuroscience,2014,15(1):125.

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