Expression and Significance of CD90 and α-SMA in Benign Bile Duct Scar

Huang Song Quan; Zhu Hong; Zhang Xiao Wen; Wu Tao; Wang Kun; Wang Lian Min; Zhao Song Ling

Volume 38 Issue 04

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Huang Song Quan , Zhu Hong , Zhang Xiao Wen , Wu Tao , Wang Kun , Wang Lian Min , Zhao Song Ling . Expression and Significance of CD90 and α-SMA in Benign Bile Duct Scar[J]. Journal of Kunming Medical University, 2017, 38(04): 40-44.

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Huang Song Quan , Zhu Hong , Zhang Xiao Wen , Wu Tao , Wang Kun , Wang Lian Min , Zhao Song Ling . Expression and Significance of CD90 and α-SMA in Benign Bile Duct Scar[J]. Journal of Kunming Medical University, 2017, 38(04): 40-44.

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Expression and Significance of CD90 and α-SMA in Benign Bile Duct Scar

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基金：国家自然科学基金资助项目(81660407);

Received Date: 2016-10-14

Abstract

Abstract

Objective To investigate the expression and significance of CD90 and α-SMA in benign bile duct scar.Methods Immunohistochemical staining of SP was applied to investigate the expression of protein CD90,α-SMA, type Ⅰ collagen, type Ⅲ collagen in 14 cases of benign bile duct scar and 10 cases of normal bile duct group.Finally image analysis was performed. Fibroblasts from the benign bile duct scar were cultivated. The expression of CD90 was measured in fibroblasts by immunofluorescence and flow cytometry. Results The expression of CD90 and α-SMA in benign bile duct scar was higher than that in normal bile duct group(P<0.05), There was positive correlation between the expression of both two in benign bile duct scar and normal bile(r=0.681,P=0.000). The expression of typeⅠcollagen and type Ⅲ collagen in benign bile duct scar was higher than that in normal bile duct group(P<0.05). There was negative correlation between the expression of both two in benign bile duct scar and normal bile(r=-0.413 P=0.011).Fibroblasts from the benign bile duct scar were cultivated and the excessive exprerssion of CD90 was found by immunofluorescence and flow cytometry. The expression of CD90, α-SMA, type Ⅰ collagen and type Ⅲ collagen were higher in benign bile duct scar than normal bile, In benign bile duct scar, the expression of CD90 and α-SMA was positively related,type Ⅰ collagen and type Ⅲ collagen was negatively related.Conclusions CD90 in fibroblasts of human benign bile duct scar is overexpressed. CD90 may be the specific phenotype of abnormal hyperplasia in benign bile duct scar.
- CD90,
- α-SMA,
- Type Ⅰ collagen,
- Type Ⅲ collagen,
- Benign bile duct scar

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[1]	[1]LILLEMOE K D,MELTON G B,CAMERON J L,et al.Postoperative bile duct strictures:Managementand outcome in the1990s[J].Ann Surg,2000,232(3):430-441.
[2]	[2]KAPLAN E P,RICHIER J C,HOWARD P S,et al.Type IIIcollagen messenger RNA is modulated in noncompliant human bladder tissue[J].J Urol,1997,157(6):2366-2369.
[3]	[3]REGE T A,HAGOOD J S.Thy-1,a versatile modulator of signaling affecting cellularadhesion,proliferation,survival,and cytokine/growth factor responses[J].Biochim Biophys Acta,2006,1763(10):991-999.
[4]	[4]PHIPPS R P,PENNEY D P,KENG P,et al.Characterization of two major populations of lung fibroblasts:Distinguishing morphology and discordant display of Thy-1and class II MHC[J].Am J Respir Cell Mol Biol,1989,1(1):65-74.
[5]赵爽,景玉婷,李为民.CD90与肿瘤关系的研究进展[J].华西医学,2015,16(12):2356-2359.
[6]杨炳林,肖坚,陈文新,等.CD90及TGF-β1在增生性瘢痕中的表达及意义[J].中华临床医师杂志,2015,8(21):4015-4017.
[7]	[7]FRIES K M,BLIEDEN T,LOONEY R J,et al.Evidence of fibroblast heterogeneity and the role of fibroblast subpopulations in fibro-sis[J].Clin Immunol Immunopathol,1994,72(3):283-292.
[8]	[8]DEAMOULIERE A,GABBIANI G.Modulation of fibroblastic cytoskeletal features during pathological situations:the role of extracellular matrix and cytokines[J].Cell Motil Cytoskeleton,1994,29(3):195-203.
[9]	[9]SERINI G,GABBIANI G.Mechanisms of myofibroblast activity and phenotypic modulation[J].Exp Cell Res,1999,250(2):273-283.
[10]唐莉,罗清礼,廖咏川,等,TGF-β1诱导CD90+和CD90一眼眶成纤维细胞亚群肌成纤维细胞转化的研究[J].实验研究,2005,1(23):6.

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