Experimental Study on Changes of EP2 Expression in the Hippocampus of Rats after Mild Brain Injury

Guo Xiao Bing; Wu De Ye; Li Heng Xi; Wu Hai Ying; Zhang Yun Xi; Yu Jian Yun; Li Ping

Volume 38 Issue 04

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Guo Xiao Bing , Wu De Ye , Li Heng Xi , Wu Hai Ying , Zhang Yun Xi , Yu Jian Yun , Li Ping . Experimental Study on Changes of EP2 Expression in the Hippocampus of Rats after Mild Brain Injury[J]. Journal of Kunming Medical University, 2017, 38(04): 5-9.

Citation:

Guo Xiao Bing , Wu De Ye , Li Heng Xi , Wu Hai Ying , Zhang Yun Xi , Yu Jian Yun , Li Ping . Experimental Study on Changes of EP2 Expression in the Hippocampus of Rats after Mild Brain Injury[J]. Journal of Kunming Medical University, 2017, 38(04): 5-9.

Citation:

Guo Xiao Bing , Wu De Ye , Li Heng Xi , Wu Hai Ying , Zhang Yun Xi , Yu Jian Yun , Li Ping . Experimental Study on Changes of EP2 Expression in the Hippocampus of Rats after Mild Brain Injury[J]. Journal of Kunming Medical University, 2017, 38(04): 5-9.

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Experimental Study on Changes of EP2 Expression in the Hippocampus of Rats after Mild Brain Injury

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基金：国家自然科学基金资助项目(31100769,30560041); 云南省教育厅科学研究基金资助项目(2017zDX164);

Received Date: 2016-12-17

Abstract

Abstract

Objective To explore the role of EP2 receptor in pathological changes of brain injury by investigating the positive expression and changes of EP2 in rat hippocampus brain regions after pure cerebral concussion( PCC). Methods The PCC rats models were established by using a self-made metal pendulum-striker concussive device. To observe the postive expression of EP2 receptor with optical density and cell count changes in CA1-4, up and down the dentate gyrus brain regins,the rats were randomly divided into 3 h, 12 h, 1 d, 2 d, 3 d and 7 d six groups(n=5) after PCC and one normal group(n=5) was used as the control.The immunohistochemistry(SP method) was used with anti-EP2 rabbit polyclonal antibody. Results The results showed that EP2 receptor immunoreactivity was lightly observed in normal group. The expression of EP2 receptor showed a significant upward trend at 3 h after PCC, peak expression appeared in 3 d group(P<0.05)and showed a downward trend in 7 d group. Conclusion The EP2 receptor induced by PCC is closely related to the pathological changes after PCC injury.
- Mild brain injury,
- Pure cerebral concussion,
- Hippocampus,
- EP2 receptor,
- Rat

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References(18)

References

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