Combined Application of TNF-α,IFN-γ and T-SPOT.TB in Identification between Active Tuberculosis Infection and Latent Tuberculosis Infection

Lv Song Qin; Zhao Hua; Bao Fu Kai; Li Xiao Fei; Yan Xin Li; Zhang Juan; Duan Hong Fen

Volume 38 Issue 04

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Lv Song Qin , Zhao Hua , Bao Fu Kai , Li Xiao Fei , Yan Xin Li , Zhang Juan , Duan Hong Fen . Combined Application of TNF-α,IFN-γ and T-SPOT.TB in Identification between Active Tuberculosis Infection and Latent Tuberculosis Infection[J]. Journal of Kunming Medical University, 2017, 38(04): 93-97.

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Combined Application of TNF-α,IFN-γ and T-SPOT.TB in Identification between Active Tuberculosis Infection and Latent Tuberculosis Infection

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基金：昆明市科技局区城创新与科技惠民基金资助项目(2014-04-A-S-02-3091);

Received Date: 2016-10-19

Abstract

Abstract

Objective To find cytokine index which can make a identification between active tuberculosis infection and latent tuberculosis infection and provide new evidence for tuberculosis diagnosis,we evaluated the levels of IL-2,IL-4,IL-6,IL-10,TNF-α and IFN-γ in people with active tuberculosis,latent tuberculosis infected people and normal people. Methods We detected the serum levels of six cytokines(IL-2, IL-4,IL-6, IL-10, TNF-α and IFN-γ) of 60 tuberculosis cases with T-SPOT.TB(+), 55 cases with latent tuberculosis infection and T-SPOT.TB(+),30 cases of normal people with T-SPOT.TB(-) by cytometric bead array(CBA). Results Compared with other groups, the levels of IL-2, IL-6, TNF-α and IFN-γ of tuberculosis(sputum culture positive) group were significantly increased(P<0.05). About IL-10,there were significant differences between two groups and the levels of IL-10 increased along with the increasing activity of tubercle bacillus and the severity of tuberculosis. TNF-α and IFN-γ of two tuberculosis groups were significantly higher than latent tuberculosis infected group and normal group(P<0.05).The serum levels of IL-4 showed no significant difference between different groups(P >0.05). Conclusions Combined with T-SPOT.TB, the detection of TNF-α and IFN-γ can make a identification between active tuberculosis infection and latent tuberculosis infection at the cytokine level, which is of great importance for the diagnosis and differentiation of latent tuberculosis infection.
- Cytokine,
- Tuberculosis,
- Latent infection,
- Diagnosis

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