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Zhang Fu Hou . Research Progress of Galectin in Mchanism of Venous Trombosis[J]. Journal of Kunming Medical University, 2016, 37(07).
Citation: Duan Jun Jun , Fang Feng , Zhang Xiao Yu , Wang Jiang , Jia Xiao , He Ying Hong . Construction of WDR5 Eukaryotic Expression Vector and Its Stable Expression in LNCaP Cells[J]. Journal of Kunming Medical University, 2017, 38(06): 1-4.
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Construction of WDR5 Eukaryotic Expression Vector and Its Stable Expression in LNCaP Cells

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基金： 国家自然科学基金资助项目 (31360288);

  • Received Date: 2017-03-19
    Abstract
  • Objective To construct a recombinant vector containing human WDR5 and establish LNCaP cell strain with stable expression of WDR5. Me thods WDR5 gene was amplified by PCR and was cloned into eukaryotic expression vector pCI-neo. The resulted pCI-neo-WDR5 plasmid was verified by restriction enzyme digestion and DNA sequencing. LNCaP cells were transfected with pCI-neo-WDR5 by liposome. The expression of WDR5 in the cells was examined by Real-time PCR. Re s ults Restriction enzyme digestion and sequencing analysis showed that WDR5 c DNA was correctly cloned into pCI-neo vector. After transfection of LNCaP cells with the recombinant plasmids, the monoclonal cell strain of stably expressed were obtained by G418 screening, which showed expressions of WDR5 m RNAs detected by Real Time-PCR. Conclus ion The recombinant of PCI-neo-WDR5 stably express in the transfected LNCaP cells, which laid a foundation for further study on WDR5.
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    • References(10)
  • [1]
    [1]TRIEVEL R C, SHILAIFAR D A.WDR5, a complexed protein[J].Nat Struct Mol Biol, 2009, 16 (8) :678-680.
    [2]
    [2]AHN Z, GUO L, WANG L, et al.Structural basis for the specific recognition of methylatedhistone H3 lysine 4 by the WD-40 protein WDR5[J].Mol Cell.2006, 22 (1) :137-144.
    [3]
    [3]WYSOCKA J, SWIGUT T, MILNE T A, et al.WDR5associates with histone H3 methylated at K4 and is essential for H3 K4 methylation and vertebrate development[J].Cell, 2005, 121 (8) :859-872.
    [4]
    [4]GE Z, SONG E J, KAWASAWA Y I, et al.WDR5 high expression and its effect on tumorigenesis in leukemia[J].Oncotarget, 2016, 7 (25) :37740-37754.
    [5]
    [5]WANG Y, LIU L, ZHONG B, et al.WDR5 is essential for assembly of the VISA-associated signaling complex and virus-triggered IRF3 and NF-kappa B activation[J].Proc Natl Acad Sci U S A, 2010, 107:815-820.
    [6]
    [6]ANG Y S, TSAIS Y, LEE D F, et al.Wdr5 mediates selfrenewal and reprogramming via the embryonic stem cell core transcriptional network[J].Cell, 2011, 145 (2) :183-197.
    [7]
    [7]CAO F, CHEN Y, CIERPICKI T, et al.An Ash2L/Rb BP5heterodimer stimulates the MLL1 methyltransferase activity through coordinated substrate interactions with the MLL1SET domain[J].PLo S One, 2010, 5 (11) :e14102.
    [8]
    [8]XU Z, GAO X, HE Y, et al.Synergistic Effect of SRY and Its Direct Target, WDR5, on Sox9 Expression[J].PLo S ONE, 2012, 7 (4) :e34327.
    [9]
    [9]XU Z, HE Y, JU J, et al.The role of WDR5 in silencing human fetal globin gene expression[J].Haematologica, 2012, 97 (11) :1632-1640.
    [10]SAMBROOK J, RUSSELL D W.分子克隆实验指南[M].第3版.北京:科技出版社, 2002:1307-1308.
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