Construction of WDR5 Eukaryotic Expression Vector and Its Stable Expression in LNCaP Cells

Duan Jun Jun; Fang Feng; Zhang Xiao Yu; Wang Jiang; Jia Xiao; He Ying Hong

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Duan Jun Jun , Fang Feng , Zhang Xiao Yu , Wang Jiang , Jia Xiao , He Ying Hong . Construction of WDR5 Eukaryotic Expression Vector and Its Stable Expression in LNCaP Cells[J]. Journal of Kunming Medical University, 2017, 38(06): 1-4.

Citation:

Duan Jun Jun , Fang Feng , Zhang Xiao Yu , Wang Jiang , Jia Xiao , He Ying Hong . Construction of WDR5 Eukaryotic Expression Vector and Its Stable Expression in LNCaP Cells[J]. Journal of Kunming Medical University, 2017, 38(06): 1-4.

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Construction of WDR5 Eukaryotic Expression Vector and Its Stable Expression in LNCaP Cells

Duan Jun Jun ^1,2,
Fang Feng ^1,2,
Zhang Xiao Yu ^1,2,
Wang Jiang ^1,2,
Jia Xiao ^1,2,
He Ying Hong ^1,2

Funds:

基金：国家自然科学基金资助项目 (31360288);

Received Date: 2017-03-19

Abstract

Abstract

Objective To construct a recombinant vector containing human WDR5 and establish LNCaP cell strain with stable expression of WDR5. Me thods WDR5 gene was amplified by PCR and was cloned into eukaryotic expression vector pCI-neo. The resulted pCI-neo-WDR5 plasmid was verified by restriction enzyme digestion and DNA sequencing. LNCaP cells were transfected with pCI-neo-WDR5 by liposome. The expression of WDR5 in the cells was examined by Real-time PCR. Re s ults Restriction enzyme digestion and sequencing analysis showed that WDR5 c DNA was correctly cloned into pCI-neo vector. After transfection of LNCaP cells with the recombinant plasmids, the monoclonal cell strain of stably expressed were obtained by G418 screening, which showed expressions of WDR5 m RNAs detected by Real Time-PCR. Conclus ion The recombinant of PCI-neo-WDR5 stably express in the transfected LNCaP cells, which laid a foundation for further study on WDR5.
- WDR5,
- Eukaryotic expression,
- G418 resistance,
- Liposome tansfection

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References(10)

References

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